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In Living Color pp 463–471Cite as

Calcium Flux Protocol: INDO-1 Stained Thymocytes

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Part of the book series: Springer Lab Manuals ((SLM))

Abstract

Calcium kinetics in cells can be measured by flow cytometry in a convenient and sensitive fashion using the UV excitable dye INDO-1. The technique relies on the emission characteristics of the fluorochrome which shifts from a blue/green higher wavelength when unbound to calcium to a purple lower wavelength when bound to calcium. This enables a ratio of lower to higher wavelenght fluorescence emisson to be generated which is indicative of the calcium flux state of the cells, and the most importantly is independant of cell size and total dye loading.

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References

  1. Grynkiewicz G, Poenie M, Tsien R.Y. A New Generation of Ca2+ Indicators with Greatly Improved Fluorescence Properties. The Journal of Biological Chemistry. 1985; 260 (6): 3400 – 3450.

    Google Scholar 

  2. June CH, Rabinovitch PS. Intracellular Ionized Calcium. In: Methods in Cell Biology. Vol 41. 2nd Edition, Part A. San Diego: Academic Press, 1994: 149 – 174.

    Google Scholar 

  3. Thastrup 0, Cullen PJ, Drobak B et al. Academic Press, 1990. PNAS 87. 2466–2470.

    Google Scholar 

  4. Waring P, Beaver J. Cyclosporin A rescues thymocytes from apoptosis induced by very low concentrations of thapsigargin: effects on mitochondrial function. Exp. Cell Res 1996; 227: 264 – 276.

    Article  CAS  Google Scholar 

  5. Haugland R.P. Handbook of fluorescent Probes. 6th Edition. Molecular Probes: 549.

    Google Scholar 

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Authors
  1. Geoffrey W. Osborne
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  2. Paul Waring
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Editors and Affiliations

  1. Division of Biology, California Institute of Technology, 1200, E. California Blvd., Pasadena, CA, 91125, USA

    Rochelle A. Diamond (Member of the Professional Staff) (Member of the Professional Staff)

  2. Optical Biology Core Facility Developmental Biology Center, University of California, Irvine, CA, 92687-2275, USA

    Susan Demaggio MS BSMT (ASCP)CQ

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© 2000 Springer-Verlag Berlin Heidelberg

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Osborne, G.W., Waring, P. (2000). Calcium Flux Protocol: INDO-1 Stained Thymocytes. In: Diamond, R.A., Demaggio, S. (eds) In Living Color. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57049-0_37

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  • DOI: https://doi.org/10.1007/978-3-642-57049-0_37

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-62978-5

  • Online ISBN: 978-3-642-57049-0

  • eBook Packages: Springer Book Archive

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