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In Living Color pp 361–371Cite as

DNA Content Determination

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Abstract

The study of the cell cycle by flow cytometry is an extremely widespread application. By staining (fixed) cells with a stoichiometrically- binding DNA fluorochrome it is possible to assess the percentages of cells in G1, S or G2/M.

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References

  1. Arndt-Jovin DJ, Jovin TM. Analysis and sorting of living cells according to deoxyribonucleic acid content. J Histochem Cytochem 1977; 25:585–589.

    Article  PubMed  CAS  Google Scholar 

  2. Crissman HA, Steinkamp JA. A new method for rapid and sensitive detection of bromodeoxyuridine in DNA-replicating cells. Exp Cell Res 1987; 173: 256–261

    Article  PubMed  CAS  Google Scholar 

  3. Dean PN et al. Cell cycle analysis using a monoclonal antibody to BrdUrd. Cell Tissue Kinet 1984; 17: 427–436.

    PubMed  CAS  Google Scholar 

  4. Gratzner H. Monoclonal antibody to 5-bromo and 5-iododeoxyuridine: a new reagent for detection of DNA replication. Science 1982; 218: 47–475.

    Article  Google Scholar 

  5. Hamilton VT, et al. Flow microfluorometric analysis of cellular DNA: critical comparison of mithramycin and propidium iodide. J Histochem Cytochem 1980; 28:1125–1128.

    Article  PubMed  CAS  Google Scholar 

  6. Karawajewl, et al. Flow sorting of hybrid hybridomas using the DNA stain Hoechst 33342. J Immunol Methods 1990; 129:277–282.

    Article  Google Scholar 

  7. Krishan A. Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide. J Cell Biol. 1975; 66:188–195.

    Article  PubMed  CAS  Google Scholar 

  8. Lydon MJ, et al. Vital DNA staining and cell sorting by flow microfluorimetry. J Cell Physiol 1980; 102:175–181.

    Article  PubMed  CAS  Google Scholar 

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Authors
  1. Derek Davies
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Editors and Affiliations

  1. Division of Biology, California Institute of Technology, 1200, E. California Blvd., Pasadena, CA, 91125, USA

    Rochelle A. Diamond (Member of the Professional Staff) (Member of the Professional Staff)

  2. Optical Biology Core Facility Developmental Biology Center, University of California, Irvine, CA, 92687-2275, USA

    Susan Demaggio MS BSMT (ASCP)CQ

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© 2000 Springer-Verlag Berlin Heidelberg

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Davies, D. (2000). DNA Content Determination. In: Diamond, R.A., Demaggio, S. (eds) In Living Color. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57049-0_29

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  • DOI: https://doi.org/10.1007/978-3-642-57049-0_29

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-62978-5

  • Online ISBN: 978-3-642-57049-0

  • eBook Packages: Springer Book Archive

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