Abstract
Stable or Transient transfection of cells with genes of interest is widely used by many investigators to study regulation of gene expression, functionally analyze proteins, and perform site directed mutation analyses of expressed proteins. By utilizing reporter genes, as discussed in the previous chapters, transfected cells can be tracked, analyzed, and isolated by flow cytometry. One of the inherent problems faced by transient and stable transfection methodology is the low frequency of tranfectants.
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References
Siebenkotten G, Petry K, Behrens-Jung U et al. Employing surface markers for the selection of transfected cells. In: Cell Separation: Methods and Applications. Marcel Dekker, in press.
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© 2000 Springer-Verlag Berlin Heidelberg
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Wilke-Douglas, M., Behrens-Jung, U. (2000). Enrichment and Selection for Reporter Gene Expression with MACSelect. In: Diamond, R.A., Demaggio, S. (eds) In Living Color. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57049-0_25
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DOI: https://doi.org/10.1007/978-3-642-57049-0_25
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-62978-5
Online ISBN: 978-3-642-57049-0
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