Abstract
The correlated data obtained from multicolour, multiparameter flow cytometric analysis provides an accurate method to characterize sub populations of cells. Multicolour immuno fluorescence is also applicable where cell numbers are very low but there is a requirement for the determination of many specific cell surface markers, such as fine needle aspirates or bone marrow specimens. Commercial flow cytometers (both the sorters and the benchtop analysers) are available with two or more lasers which permits the use of multiple excitation wavelengths and a wider range of fluorochromes.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Beavis AJ and Pennline KJ. Simultaneous measurement of five cell surface antigens by five-colour immuno fluorescence. Cytometry 15: 371–376;1994.
Beavis AJ and Pennline KJ. Allo-7: A new fluorescent tandem dye for use in flow cytometry. Cytometry 24: 390–394; 1996.
Beavis AJ and Pennline KJ. Detection of cell surface antigens using antibody-conjugated fluorospheres (ACF): Application for Six-Colour immuno fluorescence. BioTechniques 21: 498–503; 1996.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Beavis, A. (2000). Phenotypic Analysis Using Five-Colour Immunofluorescence and Flow Cytometry. In: Diamond, R.A., Demaggio, S. (eds) In Living Color. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57049-0_15
Download citation
DOI: https://doi.org/10.1007/978-3-642-57049-0_15
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-62978-5
Online ISBN: 978-3-642-57049-0
eBook Packages: Springer Book Archive