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Transgenic Beetroot (Beta vulgaris)

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Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 47))

Abstract

Beta vulgaris L. (red beet) is a vegetable grown in temperate and subfrigid zones. The tuberous root of this plant is well known as the material for food-stuff in Europe. The root has received attention because of its high content of water-soluble pigments, which belong to the betalain family. They consist of red-violet betacyanin (mainly betanin) and yellow betaxathin (mainly vulgax-anthin- I) (Fig. 1). Since the root powder of B. vulgaris is permitted as a food colorant under the 1960 Color Additive Amendment, it has been used in the food industries as a natural color additive for some food processes (von Elbe et al. 1974b).

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References

  • Bhadra R, Vani SS, Shanks JV (1993) Production of indole alkaloids by selected hairy root lines of Catharanthus roseus. Biotechnol Bioeng 41:581–592

    Article  PubMed  CAS  Google Scholar 

  • Bieleski RL (1973) Phosphate pools, phosphate transport, and phosphate availability. Annu Rev Plant Physiol 24:225–252

    Article  CAS  Google Scholar 

  • Brodelius P (1988) Permeabilization of plant cells for release of intracellularly stored products: viability studies. Appl Microbiol Biotechnol 27:561–566

    CAS  Google Scholar 

  • Curtis WR (1993) Cultivation of roots in bioreactors. Curr Opin Biotechnol 4:205–210

    Article  PubMed  CAS  Google Scholar 

  • Curtis WR, Hasegawa PM, Emery AH (1991) Modeling linear and variable growth in phosphate limited suspension cultures of opium poppy. Biotechnol Bioeng 38:371–379

    Article  PubMed  CAS  Google Scholar 

  • Dilorio AA, Cheetham RD, Weathers PI (1992a) Carbon dioxide improves the growth of hairy roots cultured on solid medium and in nutrient mists. Appl Microbiol Biotechnol 37:463–467

    Article  Google Scholar 

  • Dilorio AA, Cheetham RD, Weathers PJ (1992b) Growth of transformed roots in a nutrient mist bioreactor: reactor performance and evaluation. Appl Microbiol Biotechnol 37:457–462

    Article  Google Scholar 

  • Dilorio AA, Weathers PJ, Cheetham RD (1993) Non-lethal secondary product release from transformed root cultures of Beta vulgaris. Appl Microbiol Biotechnol 39:174–180

    Google Scholar 

  • Dougall DK (1980) Nutrition and metabolism. In: Staba EJ (ed) Plant tissue culture as a source of biochemicals. CRC Press, Boca Raton, pp 21–58

    Google Scholar 

  • Dunlop DS, Curtis WR (1991) Synergistic response of plant hairy-root cultures to phosphate limitation and fungal elicitation. Biotechnol Prog 7:434–438

    Article  CAS  Google Scholar 

  • Hamill JD, Parr AJ, Robins RJ, Rhodes MJC (1986) Secondary product formation by cultures of Beta vulgaris and Nicotiana rustica transformed with Agrobacterium rhizogenes. Plant Cell Rep 5:111–114

    Article  CAS  Google Scholar 

  • Hamill JD, Parr AJ, Rhodes MJC, Robins RJ, Walton NJ (1987) New routes to plant secondary products. Bio/Technology 5:800–804

    Article  CAS  Google Scholar 

  • Hammouri MK, Ereifej KI, Shibli RA (1998) Quantitive analysis of fructose fate in a plant fermentation system. J Agric Food Chem 46:1428–1432

    Article  CAS  Google Scholar 

  • Hempel J, Böhm H (1997) Betaxanthin pattern of hairy roots from Beta vulgaris var.lutea and its alteration by feeding of amino acid. Phytochemistry 44:847–852

    Article  CAS  Google Scholar 

  • Hilton MG, Rhodes MJC (1990) Growth and hyoscyamine production of “hairy root” cultures of Datura stramonium in a modified stirred tank reactor. Appl Microbiol Biotechnol 33:132–138

    Article  PubMed  CAS  Google Scholar 

  • Hunter CS, Kilby NJ (1990) Betanin production and release in vitro from suspension culture of Beta vulgaris. Methods Mol Biol 6:545–554

    PubMed  CAS  Google Scholar 

  • Kilby NJ, Hunter CS (1990) Repeated harvest of vacuole-located secondary product from in vitro grown plant cells using 1.02 MHz ultrasound. Appl Microbiol Biotechnol 33:448–451

    Article  CAS  Google Scholar 

  • Kilby NJ, Hunter CS (1991) Towards optimization of the use of 1.02 MHz ultrasound to harvest vacuole-located secondary product from in vitro grown plant cells. Appl Microbiol Biotechnol 34:478–480

    Article  CAS  Google Scholar 

  • Kino-oka M, Tone S (1996) Extracellular production of pigment from red beet hairy roots accompanied by oxygen starvation. J Chem Eng Jpn 29:488–493

    Article  CAS  Google Scholar 

  • Kino-oka M, Hongo Y, Taya M, Tone S (1992) Culture of red beet hairy root in bioreactor and recovery of pigment released from the cells by repeated treatment of oxygen starvation. J Chem Eng Jpn 25:490–495

    Article  CAS  Google Scholar 

  • Kino-oka M, Taya M, Tone S (1995a) Culture of red beet hairy roots in a column-type reactor associated with pigment release. Plant Tissue Cult Lett 12:201–204

    Article  CAS  Google Scholar 

  • Kino-oka M, Taya M, Tone S (1995b) Kinetic expression for pigment production in culture of red beet hairy roots. J Chem Eng Jpn 28:772–778

    Article  CAS  Google Scholar 

  • Kino-oka M, Tsutsumi S, Tone S (1996) Oxygen transfer in bioreactor for culture of plant hairy roots. J Chem Eng Jpn 29:531–534

    Article  CAS  Google Scholar 

  • Kondo O, Honda H, Taya M, Kobayashi T (1989) Comparison of growth properties of carrot hairy root in various bioreactors. Appl Microbiol Bioeng 32:291–294

    CAS  Google Scholar 

  • Mano Y (1989) Variation among hairy root clones and its application. Plant Tissue Cult Lett 6:1–9

    Article  CAS  Google Scholar 

  • McKelvey SA, Gehrig JA, Hollar KA, Curtis WR (1993) Growth of plant root cultures in liquidand gas-dispersed reactor environments. Biotechnol Prog 9:317–322

    Article  PubMed  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nakajima H, Sonomoto K, Morikawa H, Sato F, Ichimura K, Yamada Y, Tanaka A (1986) Entrapment of Lavandula vera cells with synthetic resin prepolymers and its application to pigment production. Appl Microbiol Biotechnol 24:266–270

    CAS  Google Scholar 

  • Pasch JH, von Eibe JH, Seil RJ (1975) Betalains as colorants in daily products. J Milk Food Technol 38:25–28

    CAS  Google Scholar 

  • Paul H, van Deelen JEM, Henken B (1990) Expression in vitro of resistanee of Heterodera schachtii in hairy roots of an alien monotelosomie addition plant of Beta vulgaris, transformed by Agrobacterium rhizogenes. Euphytiea 48:153–157

    Article  Google Scholar 

  • Ramakrishnan D, Curtis WR (1994) Fluid dynamies studies in plant root cultures for application to bioreactor design. In: Ryu DD, Furusaki S (eds) Advances in plant biotechnology. Elsevier, Amsterdam, pp 281–305

    Google Scholar 

  • Ramakrishnan D, Curtis WR (1995) Evaluated meristematic respiration in plant root cultures: implications to reactor design. J Chem Eng Jpn 28:491–493

    Article  CAS  Google Scholar 

  • Rodriguez-Mendiola MA, Stafford A, Cresswell R, Arias-Castro C (1992) Bioreactors for growth of plant roots. Enzyme Microb Technol 13:697–702

    Article  Google Scholar 

  • Rokem JS, Goldberg I (1985) Secondary metabolites from plant cell suspension cultures. In: Mizrahi A, Wezel AL (eds) Methods for yield improvement. Advances in biotechnological processes 4. Alan R Liss, New York, pp 241–274

    Google Scholar 

  • Tabata M, Fujita Y (1985) Production of shikonin by plant cell cultures. In: Zaitln M, Day P, Hollaender A (eds) Biotechnology in plant science. Academic Press, New York, pp 207–218

    Chapter  Google Scholar 

  • Tanaka N (1990) Detection of opines by paper electrophoresis. Plant Tissue Cult Lett 7:45–47

    Article  CAS  Google Scholar 

  • Tanaka N, Hayakawa M, Mano Y, Ohkawa H, Matsui C (1985) Infection of turnip and radish storage roots with Agrobacterium rhizogenes. Plant Cell Rep 4:74–77

    Article  Google Scholar 

  • Taya M, Mine K, Kino-oka M, Tone S, Ichi T (1992) Production and release of pigment by culture of transformed hairy root of red beet. J Ferment Bioeng 73:31–36

    Article  CAS  Google Scholar 

  • Taya M, Yakura K, Kino-oka M, Tone S (1994) Influence of medium constituents on enhancement of red beet hairy roots. J Ferment Bioeng 77:215–217

    Article  CAS  Google Scholar 

  • Tone S, Kino-oka M, Hitaka Y, Taya M (1997) High-density culture of hairy roots with a radial flow reactor. In: Nienow AW (ed) Bioreactor and bioprocess fluid dynamics. Mechanical Engineering Publications, London, pp 115–125

    Google Scholar 

  • Uozumi N, Kato Y, Nakashimada Y, Kobayashi T (1992) Excretion of peroxidase from horseradish hairy root in combination with ion supplementation. Appl Mierobiol Biotechnol 37:560–565

    CAS  Google Scholar 

  • von Eibe JH, Maing IY (1973) Betalains as possible food colorants of me at substitutes. Cereal Sci Today 18:263–265

    Google Scholar 

  • von Eibe JH, Klement JT, Amundson CH, Cassens RG, Lindsav RC (1974a) Evaluation of betalain pigments as sausage colorants. J Food Sci 39:128–132

    Article  Google Scholar 

  • von Eibe JH, Maing IY, Amundson CH (1974b) Color stability of betanin. J Food Sci 39:334–337

    Article  Google Scholar 

  • Xing T, Zhang D-Y, Hall JF, Blumwald E (1996) Auxin level and auxin binding protein availability in rol B transformed Beta vulgaris cells. Biol Plant 38:351–362

    Article  CAS  Google Scholar 

  • Zhang Q, Lauchli A, Greenway H (1992) Effect of anoxia on solute loss from beetroot storage tissue. J Exp Bot 43:897–905

    Article  CAS  Google Scholar 

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© 2001 Springer-Verlag Berlin Heidelberg

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Kino-Oka, M., Tone, S. (2001). Transgenic Beetroot (Beta vulgaris). In: Bajaj, Y.P.S. (eds) Transgenic Crops II. Biotechnology in Agriculture and Forestry, vol 47. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56901-2_5

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  • DOI: https://doi.org/10.1007/978-3-642-56901-2_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-63130-6

  • Online ISBN: 978-3-642-56901-2

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