Skip to main content

Somatic Hybridization Between Nicotiana sylvestris Speg. & Comes and N. plumbaginifolia Viv.

  • Chapter
Somatic Hybridization in Crop Improvement II

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 49))

  • 261 Accesses

Abstract

Although Nicotiana sylvestris and N. plumbaginifolia belong to the same section, viz., Alatae of the subgenus Petunioides, they differ markedly in morphology, geographic distribution, and karyotype (Goodspeed 1954). N. sylvestris is the maternal parent of cultivated allotetraploid N. tabacum (Gray et al. 1974), which is in the subgenus Tabacum, and possesses some morphological characters not present in the section Alatae. N. sylvestris occurs exclusively in northwestern Argentina, while N. plumbaginifolia has a much wider distribution, extending from northwestern Argentina to Brazil, Peru, Guatemala, Cuba, and Mexico. The chromosomes of N. sylvestris 2n=24) are rather uniform in size and are clearly bi-armed, while those of N. plumbaginifolia (2n=20) differ significantly in size and are telocentric or acrocentric (Fig. 1A,B).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Ananiev EV, Riera-Lizarazu O, Rines HW, Phillips RL (1997) Oat-maize chromosome addition lines: a new system for mapping the maize genome. Proc Natl Acad Sci USA, 94:3524–3529

    Article  PubMed  CAS  Google Scholar 

  • Ananiev EV, Phillips RL, Rines HW (1998a) Complex structure of knob DNA on maize chromosome 9: retrotransposon invasion into heterochromatin. Genetics, 149:2025–2037

    PubMed  CAS  Google Scholar 

  • Ananiev EV, Phillips RL, Rines HW (1998b) A knob-associated tandem repeat in maize capable of forming fold-back DNA segments: are chromosome knobs megatransposons? Proc Natl Acad Sci USA, 95:10785–10790

    Article  PubMed  CAS  Google Scholar 

  • Ananiev EV, Phillips RL, Rines HW (1998c) Chromosome-specific molecular organization of maize Zea mays L.) centromeric regions. Proc Natl Acad Sci USA, 95:13073–13078

    Article  PubMed  CAS  Google Scholar 

  • Belhard G, Pelletier G, Vedel F, Quetier F (1978) Morphological characteristics and chloroplast DNA distribution in different cytoplasmic parasexual hybrids of Nicotiana tabacum. Mol Gen Genet, 165:231–238

    Article  Google Scholar 

  • Bonnema AB, Melzer JM, Murray LW, O’Connel MA (1992) Nonrandom inheritance of organellar genomes in symmetric and asymmetric somatic hybrids between Lycopersicon esculentum and L. pennellii. Theor Appl Genet, 84:435–442

    Article  Google Scholar 

  • Chen CC, Huang CR, To KY (1985) Anther cultures of four diploid Nicotiana species and chromosome numbers of regenerated plants. Bot Bull Acad Sin, 26:147–153

    Google Scholar 

  • Chen K, Wildman SG, Smith HH (1977) Chloroplast DNA distribution in parasexual hybrids as shown by polypeptide composition of fraction I protein. Proc Natl Acad Sci USA, 74:5109–5112

    Article  PubMed  CAS  Google Scholar 

  • Chen ZJ, Phillips RL, Rines HW (1998) Maize DNA enrichment by representational difference analysis in a maize chromosome addition line of oat. Theor Appl Genet, 97:337–344

    Article  CAS  Google Scholar 

  • Christoff M (1928) Cytological studies in the genus Nicotiana. Genetics, 13:233–277

    PubMed  CAS  Google Scholar 

  • Cox DR, Burmeister M, Price ER, Kim S, Myers RM (1990) Radiation hybrid mapping: a somatic cell genetics method for constructing high-resolution maps of mammalian chromosomes. Science, 250:245–250

    Article  PubMed  CAS  Google Scholar 

  • Cséplö A, Nagy F, Maliga P (1984) Interspecific protoplast fusion to rescue a cytoplasmic lincomycin resistance mutation into fertile Nicotiana plumbaginifolia plants. Mol Gen Genet, 198:7–11

    Article  Google Scholar 

  • Doležel J, Lucretti S, Schubert I (1994) Plant chromosome analysis and sorting by flow cytometry. Crit Rev Plant Sci, 13:275–309

    Google Scholar 

  • Famelaer I, Gleba YY, Sidorov VA, Kaleda VA, Parakonny AS, Boryshuk NV, Chereb NN, Negrutiu I, Jacobs M (1989) Intrageneric asymmetric hybrids between Nicotiana plumbaginifolia and Nicotiana sylvestris obtained by gamma-fusion. Plant Sci, 61:105–117

    Article  Google Scholar 

  • Famelaer I, Negrutiu I, Mouras A, Vaucheret H, Jacobs M (1990) Asymmetric hybridization in Nicotiana by gamma fusion and progeny analysis of self-fertile hybrids. Theor Appl Genet, 79:513–520

    Article  Google Scholar 

  • Gleba YY, Parokonny A, Kotov I, Negrutiu I, Momot V (1987) Spatial separation of parental genomes in hybrids of somatic plant cells. Proc Natl Acad Sci USA, 84:3709–3713

    Article  PubMed  CAS  Google Scholar 

  • Goodspeed TH (1954) The genus Nicotiana. Chronica Botanica, Waltham, Massachusetts

    Google Scholar 

  • Gray JC, Kung SD, Wildman SG (1974) Origin of Nicotiana tabacum L. detected by polypeptide composition of fraction I protein. Nature, 252:226–227

    Article  PubMed  CAS  Google Scholar 

  • Huang HC, Chen CC (1988) Genome multiplication in cultured protoplasts of two Nicotiana species. J Hered, 79:28–32

    Google Scholar 

  • Hung CY, Lai YK, Feng TY, Chen CC (1993) Chloroplast segregation in somatic hybrids of Nicotiana plumbaginifolia and N. sylvestris having different ratios of parental nuclear genomes. Plant Cell Rep, 13:83–86

    Article  CAS  Google Scholar 

  • Jena KK, Khush GS (1989) Monosomie alien addition lines of rice: production, morphology, cytology, and breeding behavior. Genome, 32:449–455

    Article  Google Scholar 

  • Kao KN, Constabel F, Michayluk MR, Gamborg OL (1974) Plant protoplast fusion and growth of intergeneric hybrid cells. Planta, 120:215–227

    Article  CAS  Google Scholar 

  • Kumar A, Cocking EC, Bovenberg WA, Kool AJ (1982) Restriction endonuclease analysis of chloroplast DNA in interspecies somatic hybrids of Petunia. Theor Appl Genet, 62:377–383

    CAS  Google Scholar 

  • Lee FM, Chen CC (1990) Somatic hybridization between Nicotiana sylvestris and N. otophora without application of selection. J Hered, 81:313–317

    Google Scholar 

  • Lee FM, Chen CC (1992) Preferential recovery of somatic hybrids from protoplast fusion of two Nicotiana species in the absence of artificial selection. Taiwania, 37:11–18

    Google Scholar 

  • Lin RF, Chen CC (1990) Cytological studies of interspecific somatic hybrids in Nicotiana. Bot Bull Acad Sin, 31:179–187

    Google Scholar 

  • Medgyesy P, Menczel L, Maliga P (1980) The use of cytoplasmic streptomycin resistance: chloroplast transfer from Nicotiana tabacum into Nicotiana sylvestris, and isolation of their somatic hybrids. Mol Gen Genet, 179:693–698

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant, 15:473–497

    Article  CAS  Google Scholar 

  • Nagy JI, Maliga P (1976) Callus induction and plant regeneration from mesophyll protoplast of Nicotiana sylvestris. Z Pflanzenphysiol, 78:453–455

    Google Scholar 

  • Narayan RKJ (1987) Nuclear DNA changes, genome differentiation and evolution in Nicotiana (Solanaceae). Plant Syst Evol, 157:161–180

    Article  CAS  Google Scholar 

  • O’Connell MA, Hanson MR (1987) Regeneration of somatic hybrid plants formed between Lycopersicon esculentum and L. pennellii. Theor Appl Genet, 75:83–89

    Google Scholar 

  • Schieder O (1978) Somatic hybrids of Datura innoxia Mill + Datura stramonium L. var. tatula L. I. Selection and characterization. Mol Gen Genet, 162:113–119

    Article  Google Scholar 

  • Schieder O (1980) Somatic hybrids between a herbaceous and two tree Datura species. Z Pflanzenphysiol, 98:119–127

    Google Scholar 

  • Schwarzacher T, Leitch AR, Bennett MD, Heslop-Harrison JS (1989) In situ localization of parental genomes in a wide hybrid. Ann Bot, 64:315–324

    Google Scholar 

  • Smith HH, Kao KN, Combatti NC (1976) Interspecific hybridization by protoplast fusion in Nicotiana. J Hered, 67:123–128

    Google Scholar 

  • Struss D, Quiros CF, Robbelen G (1992) Mapping of molecular markers on Brassica B-genome chromosomes added to Brassica napus. Plant Breed, 108:320–323

    Article  CAS  Google Scholar 

  • Suen DF, Wang CK, Lin RF, Kao YY, Lee FM, Chen CC (1997) Assignment of DNA markers to Nicotiana sylvestris chromosomes using monosomic alien addition lines. Theor Appl Genet, 94:331–337

    Article  CAS  Google Scholar 

  • Sundberg E, Glimelius K (1991) Effects of parental ploidy level and genetic divergence on chromosome elimination and chloroplast segregation in somatic hybrids within Brassicaceae. Theor Appl Genet, 83:81–88

    Article  Google Scholar 

  • Sundberg E, Lagercrantz U, Glimelius K (1991) Effects of cell type used for fusion on chromosome elimination and chloroplast segregation in Brassica oleracea (+) Brassica napus hybrids. Plant Sci, 78:89–98

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2001 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Chen, CC., Kao, YY., Lee, FM., Lin, RF. (2001). Somatic Hybridization Between Nicotiana sylvestris Speg. & Comes and N. plumbaginifolia Viv.. In: Nagata, T., Bajaj, Y.P.S. (eds) Somatic Hybridization in Crop Improvement II. Biotechnology in Agriculture and Forestry, vol 49. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56758-2_20

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-56758-2_20

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-62500-8

  • Online ISBN: 978-3-642-56758-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics