Abstract
Although Nicotiana sylvestris and N. plumbaginifolia belong to the same section, viz., Alatae of the subgenus Petunioides, they differ markedly in morphology, geographic distribution, and karyotype (Goodspeed 1954). N. sylvestris is the maternal parent of cultivated allotetraploid N. tabacum (Gray et al. 1974), which is in the subgenus Tabacum, and possesses some morphological characters not present in the section Alatae. N. sylvestris occurs exclusively in northwestern Argentina, while N. plumbaginifolia has a much wider distribution, extending from northwestern Argentina to Brazil, Peru, Guatemala, Cuba, and Mexico. The chromosomes of N. sylvestris 2n=24) are rather uniform in size and are clearly bi-armed, while those of N. plumbaginifolia (2n=20) differ significantly in size and are telocentric or acrocentric (Fig. 1A,B).
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References
Ananiev EV, Riera-Lizarazu O, Rines HW, Phillips RL (1997) Oat-maize chromosome addition lines: a new system for mapping the maize genome. Proc Natl Acad Sci USA, 94:3524–3529
Ananiev EV, Phillips RL, Rines HW (1998a) Complex structure of knob DNA on maize chromosome 9: retrotransposon invasion into heterochromatin. Genetics, 149:2025–2037
Ananiev EV, Phillips RL, Rines HW (1998b) A knob-associated tandem repeat in maize capable of forming fold-back DNA segments: are chromosome knobs megatransposons? Proc Natl Acad Sci USA, 95:10785–10790
Ananiev EV, Phillips RL, Rines HW (1998c) Chromosome-specific molecular organization of maize Zea mays L.) centromeric regions. Proc Natl Acad Sci USA, 95:13073–13078
Belhard G, Pelletier G, Vedel F, Quetier F (1978) Morphological characteristics and chloroplast DNA distribution in different cytoplasmic parasexual hybrids of Nicotiana tabacum. Mol Gen Genet, 165:231–238
Bonnema AB, Melzer JM, Murray LW, O’Connel MA (1992) Nonrandom inheritance of organellar genomes in symmetric and asymmetric somatic hybrids between Lycopersicon esculentum and L. pennellii. Theor Appl Genet, 84:435–442
Chen CC, Huang CR, To KY (1985) Anther cultures of four diploid Nicotiana species and chromosome numbers of regenerated plants. Bot Bull Acad Sin, 26:147–153
Chen K, Wildman SG, Smith HH (1977) Chloroplast DNA distribution in parasexual hybrids as shown by polypeptide composition of fraction I protein. Proc Natl Acad Sci USA, 74:5109–5112
Chen ZJ, Phillips RL, Rines HW (1998) Maize DNA enrichment by representational difference analysis in a maize chromosome addition line of oat. Theor Appl Genet, 97:337–344
Christoff M (1928) Cytological studies in the genus Nicotiana. Genetics, 13:233–277
Cox DR, Burmeister M, Price ER, Kim S, Myers RM (1990) Radiation hybrid mapping: a somatic cell genetics method for constructing high-resolution maps of mammalian chromosomes. Science, 250:245–250
Cséplö A, Nagy F, Maliga P (1984) Interspecific protoplast fusion to rescue a cytoplasmic lincomycin resistance mutation into fertile Nicotiana plumbaginifolia plants. Mol Gen Genet, 198:7–11
Doležel J, Lucretti S, Schubert I (1994) Plant chromosome analysis and sorting by flow cytometry. Crit Rev Plant Sci, 13:275–309
Famelaer I, Gleba YY, Sidorov VA, Kaleda VA, Parakonny AS, Boryshuk NV, Chereb NN, Negrutiu I, Jacobs M (1989) Intrageneric asymmetric hybrids between Nicotiana plumbaginifolia and Nicotiana sylvestris obtained by gamma-fusion. Plant Sci, 61:105–117
Famelaer I, Negrutiu I, Mouras A, Vaucheret H, Jacobs M (1990) Asymmetric hybridization in Nicotiana by gamma fusion and progeny analysis of self-fertile hybrids. Theor Appl Genet, 79:513–520
Gleba YY, Parokonny A, Kotov I, Negrutiu I, Momot V (1987) Spatial separation of parental genomes in hybrids of somatic plant cells. Proc Natl Acad Sci USA, 84:3709–3713
Goodspeed TH (1954) The genus Nicotiana. Chronica Botanica, Waltham, Massachusetts
Gray JC, Kung SD, Wildman SG (1974) Origin of Nicotiana tabacum L. detected by polypeptide composition of fraction I protein. Nature, 252:226–227
Huang HC, Chen CC (1988) Genome multiplication in cultured protoplasts of two Nicotiana species. J Hered, 79:28–32
Hung CY, Lai YK, Feng TY, Chen CC (1993) Chloroplast segregation in somatic hybrids of Nicotiana plumbaginifolia and N. sylvestris having different ratios of parental nuclear genomes. Plant Cell Rep, 13:83–86
Jena KK, Khush GS (1989) Monosomie alien addition lines of rice: production, morphology, cytology, and breeding behavior. Genome, 32:449–455
Kao KN, Constabel F, Michayluk MR, Gamborg OL (1974) Plant protoplast fusion and growth of intergeneric hybrid cells. Planta, 120:215–227
Kumar A, Cocking EC, Bovenberg WA, Kool AJ (1982) Restriction endonuclease analysis of chloroplast DNA in interspecies somatic hybrids of Petunia. Theor Appl Genet, 62:377–383
Lee FM, Chen CC (1990) Somatic hybridization between Nicotiana sylvestris and N. otophora without application of selection. J Hered, 81:313–317
Lee FM, Chen CC (1992) Preferential recovery of somatic hybrids from protoplast fusion of two Nicotiana species in the absence of artificial selection. Taiwania, 37:11–18
Lin RF, Chen CC (1990) Cytological studies of interspecific somatic hybrids in Nicotiana. Bot Bull Acad Sin, 31:179–187
Medgyesy P, Menczel L, Maliga P (1980) The use of cytoplasmic streptomycin resistance: chloroplast transfer from Nicotiana tabacum into Nicotiana sylvestris, and isolation of their somatic hybrids. Mol Gen Genet, 179:693–698
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant, 15:473–497
Nagy JI, Maliga P (1976) Callus induction and plant regeneration from mesophyll protoplast of Nicotiana sylvestris. Z Pflanzenphysiol, 78:453–455
Narayan RKJ (1987) Nuclear DNA changes, genome differentiation and evolution in Nicotiana (Solanaceae). Plant Syst Evol, 157:161–180
O’Connell MA, Hanson MR (1987) Regeneration of somatic hybrid plants formed between Lycopersicon esculentum and L. pennellii. Theor Appl Genet, 75:83–89
Schieder O (1978) Somatic hybrids of Datura innoxia Mill + Datura stramonium L. var. tatula L. I. Selection and characterization. Mol Gen Genet, 162:113–119
Schieder O (1980) Somatic hybrids between a herbaceous and two tree Datura species. Z Pflanzenphysiol, 98:119–127
Schwarzacher T, Leitch AR, Bennett MD, Heslop-Harrison JS (1989) In situ localization of parental genomes in a wide hybrid. Ann Bot, 64:315–324
Smith HH, Kao KN, Combatti NC (1976) Interspecific hybridization by protoplast fusion in Nicotiana. J Hered, 67:123–128
Struss D, Quiros CF, Robbelen G (1992) Mapping of molecular markers on Brassica B-genome chromosomes added to Brassica napus. Plant Breed, 108:320–323
Suen DF, Wang CK, Lin RF, Kao YY, Lee FM, Chen CC (1997) Assignment of DNA markers to Nicotiana sylvestris chromosomes using monosomic alien addition lines. Theor Appl Genet, 94:331–337
Sundberg E, Glimelius K (1991) Effects of parental ploidy level and genetic divergence on chromosome elimination and chloroplast segregation in somatic hybrids within Brassicaceae. Theor Appl Genet, 83:81–88
Sundberg E, Lagercrantz U, Glimelius K (1991) Effects of cell type used for fusion on chromosome elimination and chloroplast segregation in Brassica oleracea (+) Brassica napus hybrids. Plant Sci, 78:89–98
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Chen, CC., Kao, YY., Lee, FM., Lin, RF. (2001). Somatic Hybridization Between Nicotiana sylvestris Speg. & Comes and N. plumbaginifolia Viv.. In: Nagata, T., Bajaj, Y.P.S. (eds) Somatic Hybridization in Crop Improvement II. Biotechnology in Agriculture and Forestry, vol 49. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56758-2_20
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DOI: https://doi.org/10.1007/978-3-642-56758-2_20
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