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Chirurgisches Forum 2001 für experimentelle und klinische Forschung pp 29–31Cite as

Inhibition von ICE (Caspase-1) induziert Zelltod in Pankreaskarzinomzellen

Inhibition of ICE (caspase-1) induces cell death in pancreatic carcinoma cells

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Part of the book series: Deutsche Gesellschaft für Chirurgie ((FORUMBAND,volume 30))

Abstract

Background: In recent studies we demonstrated the overexpression of cysteine protease ICE (caspase-1) in pancreatic carcinoma tissue and the significant correlation with factors associated with poor prognosis, pointing to possible aspects of ICE in proliferative processes in human pancreatic carcinoma. In this study we investigated the influence of ICE inhibition on the cell cycle of pancreatic carcinoma cells. Methods: For cell cycle analysis and Western blot analysis, pancreatic cells (AsPC-1, BxPC-3, MiaPaCa-2 and Panc-1) were incubated with the specific ICE-inhibitor Ac-AAVALLPAVLLALLAP-YVAD.CHO (p-YVAD.CHO) for 24 h. Results: We found that incubation with p-YVAD.CHO (25 µM) induces a non-apoptotic/necrotic-like cell death in pancreatic AsPC-1 (24.5 ± 10.3%), BxPC-3 (21.9 ± 6.9%), MiaPaCa-2 (32.0 ± 16.2%) and Panc-1 (15.3 ± 1.1%) cells. Western blot analysis revealed an ICE-dependent modulation of the expression levels of the proteins of the bcl-2 family, known to play a key role in the regulation of cell death. Conclusion Our observations support our previous findings that ICE may be involved in antiapoptotic processes in pancreatic carcinoma and may provide new aspects in its role in proliferation and cell death.

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Literatur

  1. Zeuner A, Eramo A, Peschle C, De Maria R (1999) Caspase activation without death. Cell Death Diff 6: 1075–1080

    Article  CAS  Google Scholar 

  2. Gansauge S, Gansauge F, Yang Y, Müller J, Seufferlein T, Ramadani M, Beger HG (1998) Interleukin 1β reconverting enzyme (caspase-1) is overexpressed in adenocarcinoma of the pancreas. Cancer Res 58: 2703–2706

    PubMed  CAS  Google Scholar 

  3. Schlosser S, Gansauge F, Schnelldorfer T, Ramadani M, Schwarz A, Beger, HH, Gansauge S (1999) Inhibition of EGF-induced interleukin-1β-converting enzyme expression reduces proliferation in the pancreatic carcinoma cell line AsPC-1. Cancer Research 59: 4551–4554

    PubMed  CAS  Google Scholar 

  4. Shinoura N, Yoshida Y, Asai A, Kirino T, Hamada H (1999) Reletive level of expression of bax and bcl-xL determines the cellular fate of apoptosis/necrosis induced by the overexpression of bax Oncogene 18: 5703–5713

    CAS  Google Scholar 

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Authors and Affiliations

  1. Abteilung für Allgemeinchirurgie, Universitätsklinik Ulm, Germany

    Dr. S. Schlosser, F. Gansauge, M. Ramadani, H. G. Beger & S. Gansauge

Authors
  1. Dr. S. Schlosser
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  2. F. Gansauge
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  3. M. Ramadani
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  4. H. G. Beger
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  5. S. Gansauge
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Corresponding author

Correspondence to S. Schlosser .

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Editors and Affiliations

  1. Klinikum der Stadt Ludwigshafen, Bremserstraße 79, 67063, Ludwigshafen, Germany

    K. Schönleben (Präsident des 118. Kongresses der Deutschen Gesellschaft für Chirurgie, Direktor der Chirurgischen Klinik) (Präsident des 118. Kongresses der Deutschen Gesellschaft für Chirurgie, Direktor der Chirurgischen Klinik)

  2. II. Chirurgischer Lehrstuhl Universität zu Köln, Ostmerheimer Straße 200, 51109, Köln, Germany

    E. Neugebauer (Vorsitzender der Sektion Chirurgische Forschung, Leiter der Biochemischen und Experimentellen Abteilung) (Vorsitzender der Sektion Chirurgische Forschung, Leiter der Biochemischen und Experimentellen Abteilung)

  3. Luisenstraße 58/59, 10117, Berlin, Germany

    W. Hartel (Generalsekretär der Deutschen Gesellschaft für Chirurgie) (Generalsekretär der Deutschen Gesellschaft für Chirurgie)

  4. Universität des Saarlandes, 66421, Homburg/Saar, Germany

    M. D. Menger (Direktor der Abteilung für Klinisch-Experimentelle Chirurgie) (Direktor der Abteilung für Klinisch-Experimentelle Chirurgie)

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© 2001 Springer-Verlag Berlin Heidelberg

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Schlosser, S., Gansauge, F., Ramadani, M., Beger, H.G., Gansauge, S. (2001). Inhibition von ICE (Caspase-1) induziert Zelltod in Pankreaskarzinomzellen. In: Schönleben, K., Neugebauer, E., Hartel, W., Menger, M.D. (eds) Chirurgisches Forum 2001 für experimentelle und klinische Forschung. Deutsche Gesellschaft für Chirurgie, vol 30. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56698-1_8

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  • DOI: https://doi.org/10.1007/978-3-642-56698-1_8

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-41718-7

  • Online ISBN: 978-3-642-56698-1

  • eBook Packages: Springer Book Archive

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