DNS-Profil, Laminin-5 und Cyclin A Expression als frühe Marker für Karzinomentstehung bei Colitis ulcerosa

Abstract

Introduction: DNA ploidy, laminin-5 γ2 chain immunoreactivity, and cyclin A expression were investigated as possible markers for carcinoma development in ulcerative colitis. Methods Group A consisted of 8 patients with ulcerative colitis-associated colorectal carcinomas, group B of 16 patients with long-standing ulcerative colitis and risk factors (duration of disease, extent of inflammation, epithelial dysplasias). A total of 683 paraffinembedded mucosal biopsies were evaluated for inflammatory activity, grade of dysplasia, ploidy status, laminin-5 γ2 chain and cyclin A expression. Results There was no difference between patient groups for grade of dysplasia or inflammatory activity. In group A, DNA assessments revealed more frequently aneuploid epithelial cell populations (p = 0.006). The laminin-5 γ2 chain was expressed with a higher frequency in group A (p = 0.002). The intensity of cyclin A expression was stronger in group A (p = 0.014). Conclusion: Nuclear DNA assessments, laminin-5 γ2 chain and cyclin A expression may help to identify ulcerative colitis patients with increased risk for carcinoma development.