Skip to main content

Amniotic Fluid Cells — Uncultured

  • Chapter
  • 723 Accesses

Part of the book series: Springer Lab Manuals ((SLM))

Abstract

Cytogenetic analysis of fetal cells after amniocentesis is routinely offered to women who have an increased risk of carrying a fetus with a chromosomal abnormality. Indications for fetal karyotyping include advanced maternal age, increased risk for fetal trisomy by elements of the triple screen (i.e. alphafetoprotein, human chorionic gonadotropin, and estriol), a previous pregnancy with an aneuploid fetus or potential risks due to family history.

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bryndorf T, Christensen B, Vad M, Parner J, Brocks V, Philip J (1997) Prenatal detection of chromosome aneuploidies by fluorescence in situ hybridization: experience with 2000 uncultured amniotic fluid samples in a prospective preclinical trial. Prenat Diagn 17:4, 333–341

    Article  PubMed  CAS  Google Scholar 

  • Claussen U, Ulmer R, Beinder E, Voigt H-J (1994) Six years experience with rapid karyotyping in prenatal diagnosis: correlations between phenotype detected by ultrasound and fetal karyotype. Prenat Diagn 14:113–121

    Article  PubMed  CAS  Google Scholar 

  • Eiben B, Trawicki W, Hammans W, Goebel R, Epplen J T (1998) A prospective comparative study on fluorescence in situ hybridization (FISH) of uncultured amnio-cytes and standard karyotype analysis. Prenat Diagn 18: 901–906

    Article  PubMed  CAS  Google Scholar 

  • Klinger K, Landes G, Shook D, Harvey R, Lopez L, Locke P, Lerner T, Osathanondh R, Leverone B, Houseal T, Pavelka K, Dackowski W (1992) Rapid detection of chromosome aneuploidies in uncultured amniocytes by using fluorescence in situ hybridization (FISH). Am J Hum Genet 51: 55–65

    PubMed  CAS  Google Scholar 

  • Liehr T, Starke H, Beensen V, Kähler Ch, Herbich M, Brude E, Ziegler M, Claussen U (1999) Translocation trisomy dup(2lq) and free trisomy 21 can be distinguished by interphase-FISH. Intern J MolekGenet 3: 11–14

    CAS  Google Scholar 

  • Philip J, Bryndorf T, Christensen B (1994) Prenatal aneuploidy detection in interphase cells by fluorescence in situ hybridization (FISH). Prenat Diagn 14: 1203–1215

    Article  PubMed  CAS  Google Scholar 

  • Ulmer R, Pfeiffer R-A, Kollert A, Beinder E (2000) Aneuploidiediagnostik mittels Fluoreszenz-in-situ-Hybridisierung (FISH); Stellenwert bei Schwangerschaften mit erhöhtem Risiko für Chromosomenaberrationen. Z. Geburtsh. Neonatol. 204:1–7

    CAS  Google Scholar 

  • Ward B E, Gersen S L ,Thev R, Wright M, Carelli M P (1997) Rapid prenatal diagnosis of chromosomal aneuploidies by fluorescence in situ hybridization: clinical experience on 10,000 specimens. In: Pinkel D, Gray J W, (Eds) Clinical applications of Fluorescence In Situ Hybridization, Chichester: John Wiley

    Google Scholar 

  • Ward B E, Gersen S L, Carelli M P, McGuire N M, Dackowski W R, Weinstein M, Sandlin C, Warren R, Klinger K W (1993) Rapid prenatal diagnosis of chromosomal aneuploidies by fluorescence in situ hybridization: clinical experience with 4,500 specimens. Am J Hum Genet 52: 854–865

    PubMed  CAS  Google Scholar 

  • Winsor E J T, Silver M P, Theve R, Wright M, Ward B (1996) Maternal cell contamination in uncultured amniotic fluid. Prenat Diagn 16: 49–54

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2002 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Ulmer, R. (2002). Amniotic Fluid Cells — Uncultured. In: Rautenstrauss, B.W., Liehr, T. (eds) FISH Technology. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56404-8_8

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-56404-8_8

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-47739-3

  • Online ISBN: 978-3-642-56404-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics