Abstract
Fluorescence in situ hybridization (FISH) is a precise technique to determine copy numbers of a particular sequence in individual cells with a resolution better than 30 Kb, but requires a priori knowledge of the target sequence to be analyzed. Comparative genomic hybridization (CGH) on the other hand allows a global analysis of the whole genome, but at significant loss in resolution (on the order of 10–15 Mb). DNA chip technology (so called genosensor-based or matrix-based CGH) combines the high resolution of FISH with the inherent multiplex capability of CGH, and adds simplicity to the analysis. This technology represents the latest addition to chip based genetic analyses.
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Müller, U.R., Bao, Y.P., Li, G., Xie, X., Raghavachari, N. (2002). Microarrays and DNA-Chips in the Analysis of Genetic Aberrations. In: Rautenstrauss, B.W., Liehr, T. (eds) FISH Technology. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56404-8_36
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DOI: https://doi.org/10.1007/978-3-642-56404-8_36
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