Abstract
Fluorescence in situ hybridization (FISH) technology has evolved constantly during the past decade. Nowadays, a large variety of probes detected with novel fluorescent dyes, allows the simultaneous detection of several different nucleic acid targets. A simultaneous hybridization of different probes has led to “multicolor FISH” experiments in which more than two fluorescent dyes are used for probe detection. Probes of greatly differing complexity can be combined for multicolor FISH procedures, including whole or partial chromosome painting probes, locus-specific probes (YAC, BAC, P1, cosmids) and repetitive sequences. Since only a limited number of distinct fluorescent dyes can be resolved with the standard microscope setup, combinatorial labeling schemes have been developed. In most circumstances, this wide variety of mixed colors can only be distinguished by utilisation of digital image acquisition and analysis systems. Multicolor FISH procedures are also demonstrated elsewhere in this manual describing for instance the simultaneous staining of each chromosome homologue in the human or murine chromosome complement in a different color (Chapter 22). In this chapter, protocols are presented describing the application of multicolor FISH in radiation and tumor cytogenetics for a quantification of radiation-induced chromosome aberrations and the detection of chromosomal rearrangements.
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© 2002 Springer-Verlag Berlin Heidelberg
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Zitzelsberger, H., O’Brien, B., Weier, HU. (2002). Multicolor FISH Techniques for the Detection of Inter- and Intrachromosomal Rearrangements. In: Rautenstrauss, B.W., Liehr, T. (eds) FISH Technology. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56404-8_31
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DOI: https://doi.org/10.1007/978-3-642-56404-8_31
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-47739-3
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