Abstract
The development of the “comparative genomic hybridization (CGH)” technique (Kallioniemi et al, 1992) enabled the cytogeneticist to comprehensively analyze chromosomal imbalances in entire genomes. The technique reveals chromosomal regions with relative gains or losses of DNA sequences, by comparing DNA from a test specimen (test DNA) with the DNA from a normal specimen (reference DNA). Test DNA labeled e.g. with biotin is mixed 1:1 with differently labeled reference DNA with digoxigenin and simultaneously hybridized to normal metaphase spreads. For the detection of the hybridized test and reference DNA, different fluorochromes are used, and each is visualized by epifluorescence microscopy with selective filters. Regions within the tumor that are over- or underrepresented in the probe mixture can be identified by an increased or decreased color ratio of the two fluorochromes.
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© 2002 Springer-Verlag Berlin Heidelberg
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Jung, V., Weber, G.A. (2002). Preparation of Samples for CGH by DOP-PCR from Cryofixed or Paraffin-Embedded, Microdissected Cells. In: Rautenstrauss, B.W., Liehr, T. (eds) FISH Technology. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56404-8_15
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DOI: https://doi.org/10.1007/978-3-642-56404-8_15
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