Molecular Cell Biology: Mechanisms and Regulation of Protein Import into the Plant Cell Nucleus

  • Vera Hemleben
  • Katrin Hinderhofer
  • Ulrike Zentgraf
Part of the Progress in Botany book series (BOTANY, volume 63)


Plant cells contain three genetically active compartments embedded in the cytoplasm which are acting in strictly regulated cooperation (Sitte 1998). Translation of proteins from organelle-encoded genes occurs in the respective semiautonomous plastid or mitochondrial compartments (stroma and matrix, respectively). Nuclear-encoded genes are transcribed in the cell nucleus, and posttranscriptionally modified mRNA, tRNA and preformed ribosomes are transported to the cytoplasm. The nuclear mRNA is translated into polypeptides either directly at free cytoplasmic ribosomes or at ribosomes associated with the rough endoplasmic reticulum (ER). Proteins necessary for the correct functioning of the cell have to be transported to the respective locations. Characteristic signal- or transit-peptides at the N-terminus of specific proteins mediate the correct transport to the plasma membrane or into the chloroplasts and mitochondria; other characteristic short motifs were found in proteins targeted to the vacuole, peroxisomes or into the cell nucleus.


Nuclear Localization Signal Nuclear Import Nuclear Pore Complex Xanthomonas Campestris Molecular Cell Biology 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Ach RA, Gruissem W (1994) A small GTP-binding protein from tomato suppresses a Schizosaccharomyces pombe cell-cycle mutant. Proc Natl Acad Sci USA 91:5863–5867PubMedCrossRefGoogle Scholar
  2. Adam SA, Marr RS, Gerace L (1990) Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors. J Cell Biol 11:807–816CrossRefGoogle Scholar
  3. Ahmad M, Jarillo JA, Smirnova O, Cashmore AR (1998) The CRY1 blue light photoreceptor of Arabidopsis interacts with phytochrome A in vitro. Mol Cell 1:939–948PubMedCrossRefGoogle Scholar
  4. Ballare CL (1999) Keeping up with the neighbours: phytochrome sensing and other signalling mechanisms. Trends Plant Sci 4:97–102PubMedCrossRefGoogle Scholar
  5. Barth AL, Nathke IS, Nelson WJ (1997) Cadherins, catenins and APC protein: interplay between cytoskeletal complexes and signaling pathways. Curr Opin Cell Biol 9:683–690PubMedCrossRefGoogle Scholar
  6. Becker SJC, Craig EA (1994) Heat-shock proteins as molecular chaperones. Eur J Biochem 219:11–23PubMedCrossRefGoogle Scholar
  7. Bonas U (2000) High-resolution genetic mapping of the pepper resistance locus Bs3 governing recognition of the Xanthomonas campestris pv. vesicatora AvrBs3 protein. Theor Appl Genet 101:255–263CrossRefGoogle Scholar
  8. Bonas U, Van den Ackerveken G, Büttner D, Hahn K, Marois E, Nennstiel D, Noël L, Rossier O, Szurek B (2000) How the bacterial plant pathogen Xanthomonas campestris pv. vesicatora conquers the host. Mol Plant Pathol 1:73–76PubMedCrossRefGoogle Scholar
  9. Borg S, Brandstrup B, Jenson TJ, Poulsen C (1997) Identification of new protein species amount 33 different small GTP-binding proteins encoded by cDNAs from Lotus ja-ponicus t and expression of corresponding mRNAs in developing root nodules. Plant J 11:237–250PubMedCrossRefGoogle Scholar
  10. Briggs WR, Huala E (1999) Blue-light photoreceptors in higher plants. Annu Rev Cell Dev Biol 15:33–62PubMedCrossRefGoogle Scholar
  11. Cashmore AR, Jarillo JA, Wu YJ, Liu D (1999) Cryptochromes: blue light receptors for plants and animals. Science 284:760–765PubMedCrossRefGoogle Scholar
  12. Choi G, Yi H, Lee J, Kwon YK, Soh MS, Shin B, Luka Z, Hahn TR, Song PS (1999) Phytochrome signalling is mediated through nucleoside diphosphate kinase2. Nature 401:610–613PubMedCrossRefGoogle Scholar
  13. Chytilova E, Macas J, Siwilinska E, Rafelski SM, Lambert GM, Galbraith DW (2000) Nuclear dynamics in Arabidopsis thaliana. Mol Biol Cell 11:2733–2741PubMedGoogle Scholar
  14. Citovsky V, Zupan J, Warnick D, Zambryski P (1992) Nuclear localization of Agrobacterium VirE2 protein in plant cells. Science 256:1802–1805PubMedCrossRefGoogle Scholar
  15. Dabauvalle MC, Benevente R, Chaly N (1988) Monoclonal antibodies to a Mr 68,000 pore complex glycoprotein interfere with nuclear protein uptake in Xenopus oocytes. Chromsoma 97:193–197CrossRefGoogle Scholar
  16. Davis LI (1995) The nuclear pore complex. Annu Rev Biochem 64:865–896PubMedCrossRefGoogle Scholar
  17. Deng XW, Quail PH (1992) Genetic and phenotypic characterization of copl mutants of Arabidopsis thaliana. Plant J 2:83–95CrossRefGoogle Scholar
  18. Deng XW, Dubiel W, Wei N, Hofmann K, Mundt K, Colicelli J, Kato J, Naumann M, Segal D, Seeger M, Glickman M Chamovitz DA, Carr A (2000) Unified nomenclature for COP9 signalosome an its subunits: an essential regulator of development. Trends Genet 16:202–203PubMedCrossRefGoogle Scholar
  19. Deshaies RJ, Meyerowitz E (2000) COP1 patrols the night beat. Nat Cell Biol 2:E102-E104PubMedCrossRefGoogle Scholar
  20. Diener TO (1995) Viroids and the nature of viroid diseases. Arch Virol Suppl 15:203–220Google Scholar
  21. Diener TO (1999) Origin and evolution of viroids and viroid- like satellite RNAs. Virus Genes 11:119–131CrossRefGoogle Scholar
  22. Dingwall C, Laskey RA (1991) Nuclear targeting sequences-a consensus? Trends Biochem Sci 16:478–481PubMedCrossRefGoogle Scholar
  23. Doye V, Hurt EC (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9:401 411Google Scholar
  24. Fankhauser C, Chory J (1997) Light control of plant development. Annu Rev Cell Dev Biol 13:203–229PubMedCrossRefGoogle Scholar
  25. Fankhauser C, Yeh KC, Lagarias JC, Zhang H, Elich TD, Chory J (1999) PKS1, a substrate phosphorylated by phytochrome that modulates light signaling in Arabidopsis. Science 284:1539–1541PubMedCrossRefGoogle Scholar
  26. Feys BJ, Parker JE (2000) Interplay of signaling pathways in plant disease resistance. Trends Genet 16:448–445CrossRefGoogle Scholar
  27. Forbes DJ (1992) Structure and function of the nuclear pore complex. Annu Rev Cell Biol 8:495–527PubMedCrossRefGoogle Scholar
  28. Foster R, Izawa T, Chua NH (1994) Plant bZIP proteins gather at ACGT elements. FASEB J 8:192–200PubMedGoogle Scholar
  29. Furuya M, Schäfer E (1996) Photoperception and signalling of induction reactions by different phytochromes. Trends Plant Sci 1:301–307Google Scholar
  30. Furuya M, Song PS (1994) Assembly and properties of holophytochrome. In: Kendrick RE, Kronenberg GHM (eds) Photomorphogenesis in plants. Kluwer, Dordrecht, pp 105–134Google Scholar
  31. Garcia-Bustos J, Heitman J, Hall MN (1991) Nuclear protein localization. Biochem Biophys Acta 1071:83–101PubMedGoogle Scholar
  32. Gil P, Kircher S, Adam E, Bury E, Kozma-Bognar L, Schäfer E, Nagy F (2000) Photocon- trol of subcellular partitioning of phytochrome-B: GFP fusion protein in tobacco seedlings. Plant J 22:135–145PubMedCrossRefGoogle Scholar
  33. Goday A, Jensen AB, Culianez-Marcia FA, Alba MM, Figueras M, Serratosa J, Torrent M, Pages M (1994) The maize abscisic acid-responsive protein Rab17 is located in thenucleus and interacts with nuclear localization signals. Plant Cell 6:351–360PubMedGoogle Scholar
  34. Görlich D (1998) Transport into and out of the cell nucleus. EMBO J 17:2721–2727PubMedCrossRefGoogle Scholar
  35. Görlich D, Kutay U (1999) Transport between the cell nucleus and the cytoplasm. Annu Rev Cell Dev Biol 15:607–660PubMedCrossRefGoogle Scholar
  36. Görlich D, Prehn S, Laskey RA, Hartmann E (1994) Isolation of a protein that is essential for the first step of nuclear protein import. Cell 79:767–778PubMedCrossRefGoogle Scholar
  37. Görlich D, Kostka S, Kraft R, Dingwall C, Laskey RA, Hartmann E, Prehne S (1995) Two different subunits of importin cooperate to recognize nuclear localization signals and bind them to the nuclear envelope. Curr Biol 5:383–392PubMedCrossRefGoogle Scholar
  38. Görlich D, Pante N, Kutay U, Aebi U, Bischoff F R (1996) Identification of different roles for RanGDP and RanGTP in nuclear protein import. EMBO J 15:5584–5594PubMedGoogle Scholar
  39. Goldberg MW, Cronshaw JM, Kiseleva E, Allen TD (1999) Nuclear-pore-complex dynamics and transport in higher eukaryotes. Protoplasma 209:144–156CrossRefGoogle Scholar
  40. Guo H, Duong H, Ma N, Lin C (1999) The Arabidopsis blue light receptor cryptochrome 2 is a nuclear protein regulated by a blue light-dependent post-transcriptional mechanism. Plant J 19:279–287PubMedCrossRefGoogle Scholar
  41. Guralnick B, Thomsen G, Citovsky V (1996) Transport of DNA into the nuclei of Xenopus oocytes by a modified VirE2 protein of Agrobacterium. Plant Cell 8:363–373PubMedGoogle Scholar
  42. Haizel T, Merkle T, Pay A, Fejes E, Nagy F (1997) Characterization of proteins that interact with GTP-bound form of the regulatory GTPase Ran in Arabidopsis. Plant J 11:93–103PubMedCrossRefGoogle Scholar
  43. Harter K, Kircher S, Frohnmeyer H, Krenz M, Nagy F, Schäfer E (1994) Light-regulated modification and nuclear translocation of cytosolic G-box binding factors in parsley. Plant Cell 6:545–559PubMedGoogle Scholar
  44. Heese-Peck A, Raikhel NV (1998a) The nuclear pore complex. Plant Mol Biol 38:145–162PubMedCrossRefGoogle Scholar
  45. Heese-Peck A, Raikhel NV (1998b) A glycoprotein modified with terminal N-acetylgluco-samine and localized at the nuclear rim shows sequence similarity to aldose-1-epimerases. Plant Cell 10:599–612.PubMedGoogle Scholar
  46. Heese-Peck A, Cole RN, Borhsenious ON, Hart GW, Raikhel NV (1995) Nuclear pore complex proteins from higher plants are modified by novel O-linked oligosaccharides. Plant Cell 7:1459–1471PubMedGoogle Scholar
  47. Hicks GR, Raikhel NV (1995a) Nuclear localization signal binding proteins in higher plant nuclei. Proc Natl Acad Sci USA 92:734–738PubMedCrossRefGoogle Scholar
  48. Hicks GR, Raikhel NV (1995b) Protein import into the nucleus: an integrated view. Annu Rev Cell Biol 11:155–188CrossRefGoogle Scholar
  49. Hicks, GR, Smith HMS, Shieh M, Raikhel NV (1995) Three classes of nuclear import signals bind to plant nuclei. Plant Physiol 107:1055–1058PubMedCrossRefGoogle Scholar
  50. Hicks GR, Smith HMS, Lobreaux S, Raikhel NV (1996) Nuclear import in permeabilised protoplasts from higher plants has unique features. Plant Cell 8:1337–1352PubMedGoogle Scholar
  51. Holm M, Deng XW (1999) Structural organization and interactions of COP1, a light-regulated developmental switch. Plant Mol Biol 41:151–158PubMedCrossRefGoogle Scholar
  52. Hübner S, Smith HMS, Hu W, Chan CK, Rihs HP, Paschal BM, Raihkel NV, Jans DA (1999) Plant importin a binds nuclear localization sequences with high affinity and can mediate nuclear import independent of importin ß. J Biol Chem 274:22610–22617PubMedCrossRefGoogle Scholar
  53. Huq E, Tepperman JM, Quail PH (2000) GIGANTEA is a nuclear protein involved in phytochrome signaling in Arabidopsis. Proc Natl Acad Sci USA 97:9789–9794PubMedCrossRefGoogle Scholar
  54. Iovine MK, Watkins JL, Wente SR (1995) The GLFG repetitive region of the nucleoporin Nupll6p interacts with Kap95p, an essential yeast nuclear import factor. J Cell Biol 131:1699–1713PubMedCrossRefGoogle Scholar
  55. Jensen AB, Goday A, Figueras M, Jessop AC, Pages M (1998) Phosphorylation mediates the nuclear targeting of the maize Rab17 protein. Plant J 13:691–697PubMedCrossRefGoogle Scholar
  56. Jiang CJ, Imamoto N, Matsuki R, Yoneda Y, Yamamoto N (1998a) Functional characterization of a plant importin a homologue — nuclear localization signal (NLS)-selective binding and mediation of nuclear import of NLS proteins in vitro. J Biol Chem 272:24083–24087CrossRefGoogle Scholar
  57. Jiang CJ, Imamoto N, Matsuki R, Yoneda Y, Yamamoto N (1998b) In vitro characterization of rice importin beta 1: molecular interaction with nuclear transport factors and mediation of nuclear protein import. FEBS Lett 437:127–130PubMedCrossRefGoogle Scholar
  58. Kendrick RE, Kronenberg GHM (1994) Photomorphogenesis in plants. Kluwer, DordrechtGoogle Scholar
  59. Kim L, Kircher S, Toth R, Adam E, Schäfer E, Nagy F (2000) Light-induced nuclear import of phytochrome-A:GFP fusion proteins is differentially regulated in transgenic tobacco and Arabidopsis. Plant J 22:125–133PubMedCrossRefGoogle Scholar
  60. Kircher S, Ledger S, Hayashi H, Weisshaar B, Schäfer E, Frohnmeyer H (1998) CPRF4a, a novel plant bZIP protein of the CPRF family: comparative analyses of light-dependent expression, post-transcriptional regulation, nuclear import and heterodimerisation. Mol Gen Genet 257:595–605PubMedCrossRefGoogle Scholar
  61. Kircher S, Kozma-Bognar L, Kim L, Adam E, Harter K, Schäfer E, Nagy F (1999a) Light quality-dependent nuclear import of the plant photoreceptors phytochrome A and B. Plant Cell 11:1445–1456PubMedGoogle Scholar
  62. Kircher S, Wellmer F, Nick P, Rügner A, Schäfer E, Harter K (1999b) Nuclear import of the parsley bZIP transcription factor CPRF2 is regulated by phytochrome photoreceptors. J Cell Biol 144:201–211PubMedCrossRefGoogle Scholar
  63. Kleiner O, Kircher S, Harter K, Batschauer A (1999) Nuclear localization of the Arabidopsis blue light receptor cryptochrome 2. Plant J 19:289–296PubMedCrossRefGoogle Scholar
  64. Klimczak LJ, Schindler U, Cashmore AR (1992) DNA binding activity of the Arabidopsis G-box binding factor GBF1 is stimulated by phosphorylation by caScin kinase II from broccoli. Plant Cell 4:87–98PubMedGoogle Scholar
  65. Klimczak LJ, Collinge MA, Farini D, Giuliano G, Walker JC, Cashmore AR (1995) Re-constitution of Arabidopsis caScin kinase II from recombinant subunits and phosphorylation of transcription factor GBF1. Plant Cell 7:105–115PubMedGoogle Scholar
  66. Knorpp C, Hugosson M, Sijoling S, Eriksson AC, Glaser E (1994) Tissue-specific differences of the mitochondrial protein import machinery: in vitro import, processing and degradation of the pre-fb subunit of the ATPase in spinach leaves and root mitochondria. Plant Mol Biol 26:571–579PubMedCrossRefGoogle Scholar
  67. Kozma-Bognar L, Hall A, Adam E, Thain SC, Nagy F, Millar AJ (1999) The circadian clock controls the expression pattern of the circadian input photoreceptor, phytochrome B. Proc Natl Acad Sci USA 96:14652–14657CrossRefGoogle Scholar
  68. Kunik T, Palanichelvam K, Czosnek H, Citovsky V, Gafni Y (1998) Nuclear import of the capsid protein of tomato yellow leaf curly virus (TYLCV) in plant and insect cells. Plant J 13:393–399Google Scholar
  69. Kwok SF, Piekos B, Misera S, Deng XW (1995) A complement often essential and plei-otropic Arabidopsis COP/DET/FUS genes is necessary for repression of photomorphogenesis in darkness. Plant Physiol 110:731–742CrossRefGoogle Scholar
  70. Landschulz WH, Johnson PF, McKnight SL (1988) The leucine zipper: a hypothetical structure common to a new class of DNA-binding proteins. Science 240:1759–1764PubMedCrossRefGoogle Scholar
  71. Lasarowitz SG (1988) Infectivity and complete nucleotide sequence of the genome of a South African isolate of maize streak virus. Nucleic Acids Res 16:229–249CrossRefGoogle Scholar
  72. Lasarowitz SG, Pinder AJ, Damsteegt VD, Rogers SG (1989) Maize streak virus genes essential for systemic spread and symptom development. EMBO J 8:1023–1032Google Scholar
  73. Leheny EA, Theg SM (1994) Apparent inhibition of chloroplast protein import by cold temperature is due to energetic considerations, not to membrane fluidity. Plant Cell 6:427–437PubMedGoogle Scholar
  74. Ligterink W, Kroj T, zur Nieden U, Hirt H (1997) Receptor-mediated activation of a MAP kinase in pathogen defense of plants. Science 276:2054–2057PubMedCrossRefGoogle Scholar
  75. Liu H, Boulton MI, Thomas CL, Prior DAM, Oparka KJ, Davies JW (1999) Maize streak virus coat protein is karyophilic and facilitates nuclear transport of viral DNA. Mol Plant-Microbe Interact 12:894–900PubMedCrossRefGoogle Scholar
  76. Liu L, White MJ, MacRae TH (1999) Transcription factors and their genes in higher plants. Functional domains, evolution and regulation. Eur J Biochem 262:247–257PubMedCrossRefGoogle Scholar
  77. Lyck R, Harmening U, Höhfeld I, Treuter E, Scharf KD, Nover L (1997) Intracellular distribution and identification of the nuclear localization signals of two plant heat-stress transcription factors. Planta 202:117–125PubMedCrossRefGoogle Scholar
  78. Martinez-Garcia JF, Huq E, Quail PH (2000) Direct targeting of light signals to a promoter element-bound transcription factor. Science 288:859–863PubMedCrossRefGoogle Scholar
  79. Mathews S, Sharrock RA (1997) Phytochrome gene diversity. Plant Cell Environ 20:666–671CrossRefGoogle Scholar
  80. Matsui M, Stoop CD, von Arnim AG, Arnim N, Deng XW (1995) Arabidopsis COP1 protein specifically interacts in vitro with a cytoskeleton-associated protein, CIP1. Proc Natl Acad Sci USA 92:4239–4243PubMedCrossRefGoogle Scholar
  81. Mattaj IW, Engelmeier L (1998) Nucleoplasms transport: the soluble phase. Annu Rev Biochem 67:265–306PubMedCrossRefGoogle Scholar
  82. McGonigle B, Bouhidel K, Irish VF (1996) Nuclear localization of the Arabidopsis APATALA3 and PISTILLATA homeotic gene products depends on their simultaneous expression. Genes Dev 10:1812–1821PubMedCrossRefGoogle Scholar
  83. McNellis TW, von Arnim AG, Araki T, Komeda Y, Misera S, Deng XW (1994) Genetic and molecular analysis of an allelic series of cop 1 mutants suggests functional roles for the multiple protein domains. Plant Cell 6:487–500PubMedGoogle Scholar
  84. Melchior F, Paschal B, Evans E, Gerace L (1993) Inhibition of nuclear protein import by nonhydrolyzable analogs of GTP and identification of the small GTPase Ran/TC4 as an essential transport factor. J Cell Biol 135:1457–1470Google Scholar
  85. Merkle T, Nagy F (1997) Nuclear import of proteins: putative import factors and development of in vitro import systems in higher plants. Trends Plant Sci 2:458–464CrossRefGoogle Scholar
  86. Merkle T, Haizel T, Matsumoto T, Harter K, Dallmann G, Nagy F (1994) Phenotype of the fission yeast cell cycle regulatory mutant pirn 1–46 is suppressed by a tobacco cDNA encoding a small, Ran-like GTP-binding protein. Plant J 6:555–565PubMedCrossRefGoogle Scholar
  87. Merkle T, Leclerc D, Marshallsay C, Nagy F (1996) A plant in vitro system for nuclear import of proteins. Plant J 10:1177–1186PubMedCrossRefGoogle Scholar
  88. Meshi T, Iwabuchi M (1995) Plant transcription factors. Plant Cell Physiol 36:1405–1420PubMedGoogle Scholar
  89. Misera S, Müller AJ, Weiland-Heidecker U, Jürgens G (1994) The FUSCA genes of Arabidopsis: negative regulators of light responses. Mol Gen Genet 244:242–252PubMedCrossRefGoogle Scholar
  90. Moore MS, Blobel G (1993) The GTP-binding protein Ran/TC4 is required for protein import into the nucleus. Nature 365:143–148CrossRefGoogle Scholar
  91. Mudgett MB, Chesnokova O, Dahlbeck D, Clark E, Bonas U, Staskawicz BJ (2000) Molecular signals required for type III secretion and translocation of the Xanthomonas campestris AvrBs2 protein to pepper plants. Proc Natl Acad Sci USA 97:13324–13329PubMedCrossRefGoogle Scholar
  92. Nagatani A (2000) Lighting up the nucleus. Science 288:821–822PubMedCrossRefGoogle Scholar
  93. Nagy F, Schäfer E (1999) Phytochromes, pif3 and light signaling go nuclear. Trends Plant Sci 4:125–126CrossRefGoogle Scholar
  94. Nagy F, Schäfer E (2000) Nuclear and cytosolic events of light-induced, phytochrome-regulated signalling in higher plants. EMBO J 19:157–163PubMedCrossRefGoogle Scholar
  95. Neer EJ, Schmidt CJ, Nambudripad R, Smith TF (1994) The ancient regulatory-protein family of WD-repeat proteins. Nature 371:297–300PubMedCrossRefGoogle Scholar
  96. Nemeth K, Salcher K, Putnoky P, Bhalerao R, Koncz-Kalman Z, Stankovic-Stangeland B, Bako L, Mathur J, ökresz L, Stabel S, Geigenberger P, Stitt M, Redei GP, Schell J, Koncz C (1998) Pleiotropic control of glucose and hormone responses by PRL1, a nuclear WD protein, in Arabidopsis. Genes Dev 12:3059–3073PubMedCrossRefGoogle Scholar
  97. Nigg EA (1997) Nucleoplasmic transport: signals, mechanisms and regulation. Nature 386:779–787PubMedCrossRefGoogle Scholar
  98. Nover L, Scharf KD (1997) Heat stress proteins and transcription factors. Cell Mol Life Sci 53:80–103PubMedCrossRefGoogle Scholar
  99. Ogura T, Tanaka N, Yabe N, Komatsu S, Hasunuma K (1999) Characterization of protein complexes containing nucleoside diphosphate kinase with characteristics of light signal transduction through phytochrome in etiolated pea seedlings. Photochem Photobiol 69:397–403CrossRefGoogle Scholar
  100. Osterlund MT, Deng XW (1998) Multiple photoreceptors mediate the light-induced reduction of GUS-COP1 from Arabidopsis hypocotyl nuclei. Plant J 16:201–208PubMedCrossRefGoogle Scholar
  101. Park DH, Somers DE, Kim YS, Choy YH, Lim HK, Soh MS, Kim HJ, Kay SA, Nam HG (1999) Control of circadian rhythms and photoperiodic flowering by the Arabidopsis GIGANTEA gene. Science 285:1579–1582PubMedCrossRefGoogle Scholar
  102. Pascal E, Sanderfoot AA, Ward BM, Medville R, Turgeon R, Lasarowitz SG (1994) The geminivirus BRI movement protein binds single- and double-stranded DNA and localizes to the nucleus. Plant Cell 6:995–1006PubMedGoogle Scholar
  103. Peracchia G, Jensen AB, Culianez-Macia FA, Grosset J, Goday A, Issinger OG, Pages M (1999) Characterization, subcellular localization and nuclear targeting of caScin kinase 2 from Zea mays. Plant Mol Biol 40:199–211PubMedCrossRefGoogle Scholar
  104. Qin S, Ward BM, Lazarowitz, SG (1998) The bipartite geminivirus coat protein aids BRI function in viral movement by affecting the accumulation of viral single-stranded DNA. J Virol 72:9247–9256PubMedGoogle Scholar
  105. Quail PH, Boylan MT, Parks BM, Short TW, Xu Y, Wagner D (1995) Phytochromes: photosensory perception and signal transduction. Science 268:675–680PubMedCrossRefGoogle Scholar
  106. Radu A, Blobel G, Moore MS (1995) Identification of a protein complex that is required for nuclear protein import and mediates docking of the import substrate to distinct nucleoporins. Proc Natl Acad Sci USA 92:1769–1773PubMedCrossRefGoogle Scholar
  107. Reichelt R, Holzenburg A, Buhle EL, Jarnik M, Engel A, Aebi U (1990) Correlation between structure and mass distribution of the nuclear pore complex, and distinct pore complex components. J Cell Biol 110:883–894PubMedCrossRefGoogle Scholar
  108. Relic B, Andjelkovic M, Rossi L, Nagamine Y, Hohn B (1998) Interaction of the DNA modifying proteins VirDl and VirD2 of Agrobacterium tumefaciens: analysis by subcellular localization in mammalian cells. Proc Natl Acad Sci USA 95:9105–9110PubMedCrossRefGoogle Scholar
  109. Rout MP, Blobel G (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123:771–783PubMedCrossRefGoogle Scholar
  110. Rout MP, Wente SR (1994) Pores for thought: nuclear pore complex proteins. Trends Cell Biol 4:357–365PubMedCrossRefGoogle Scholar
  111. Saalbach G, Christov V (1994) Sequence of a plant cDNA from Vicia faba encoding a novel Ran-related GTP-binding protein. Plant Mol Biol 24:969–972PubMedCrossRefGoogle Scholar
  112. Sakamoto K, Nagatani A (1996) Nuclear localization activity of phytochrome B. Plant J 10:859–868PubMedCrossRefGoogle Scholar
  113. Sanderfoot AA, Ingham DJ, Lazarowitz SG (1996) A viral movement protein as a nuclear shuttle. Plant Physiol 110:23–33PubMedCrossRefGoogle Scholar
  114. Schäfer E, Marchai B, Marme D (1972) In vivo measurements of phytochrome photostationary state in far-red light. Photochem Photobiol 15:457–464CrossRefGoogle Scholar
  115. Scharf KD, Materna T, Treuter E, Nover L (1994) Heat stress promoters and transcription factors. In: Nover L (ed) Plant promoters and transcription factors. Springer, Berlin Heidelberg New York, pp 125–162Google Scholar
  116. Scharf KD, Heider H, Höhfeld I, Lyck R, Schmidt E, Nover L (1998) The tomato Hsf system: HsfA2 needs interaction with Hsf Al for efficient nuclear import and may be localized in cytoplasmic heat stress granules. Mol Cell Biol 18:2240–2251PubMedGoogle Scholar
  117. Schledz M, Leclerc D, Neuhaus G, Merkle T (1998) Characterization of four cDNAs encoding different importin alpha homologs from Arabidopsis. Plant Physiol 116:868Google Scholar
  118. Schöffl F, Prändl R, Reindl A (1999) Molecular responses to heat stress. In: Shinozaki K, Yamaguschi-Shinozaki K (eds) Molecular responses to cold, drought, heat and salt stress in higher plants. RG Landes Company, Austin, TX, pp 81–98Google Scholar
  119. Scofield GN, Beven AF, Shaw PJ, Doonan JH (1992) Identification and localization of a nucleoporin-like protein component of the plant nuclear matrix. Planta 187:414–420CrossRefGoogle Scholar
  120. Sheldon LA, Kingston RE (1993) Hydrophobic coiled-coil domains regulate the subcellular localization of the human heat shock factor 2. Genes Dev 7:1549–1558PubMedCrossRefGoogle Scholar
  121. Shieh MW, Wessler SR, Raikhel NV (1993) Nuclear targeting of the maize R protein requires two nuclear localization sequences. Plant Physiol 101:353–361PubMedCrossRefGoogle Scholar
  122. Shoji K, Iwasaki T, Matsuki R, Miyao M, Yamamoto N (1998) Cloning of a cDNA encoding an importin a and down-regulation of the gene by light in rice leaves. Gene 212:279–286PubMedCrossRefGoogle Scholar
  123. Sitte P (1998) Facts and concepts in cell compartmentation. Prog Bot 59:3–45CrossRefGoogle Scholar
  124. Smeekens S (2000) Sugar-induced signal transduction in plants. Annu Rev Plant Physiol Plant Mol Biol 51:49–81PubMedCrossRefGoogle Scholar
  125. Smith HMS, Raikhel NV (1998) Nuclear localization signal receptor importin a associates with the cytoskeleton. Plant Cell 10:1791–1799PubMedGoogle Scholar
  126. Smith HMS, Raikhel NV (1999) Protein targeting to the nuclear pore. What can we learn from plants? Plant Physiol 119:1157–1163.PubMedCrossRefGoogle Scholar
  127. Smith HMS, Hicks GR, Raikhel NV (1997) Importin a from Arabidopsis thaliana is a nuclear import receptor that recognises three classes of import signals. Plant Physiol 114:411–417PubMedCrossRefGoogle Scholar
  128. Smith TF, Gaitatzes C, Saxena K, Neer EJ (1999) The WD repeat: a common architecture for diverse functions. Trends Biochem Sci 24:181–185PubMedCrossRefGoogle Scholar
  129. Somers DE, Devil PF, Kay SA (1998) Phytochromes and cryptochromes in the entrain-ment of the Arabidopsis circadian clock. Science 282:1488–1490PubMedCrossRefGoogle Scholar
  130. Stacey MG, von Arnim AG (1999) A novel motive mediates the targeting of the Arabidopsis COP1 protein to subnuclear foci. J Biol Chem 274:27231–27236PubMedCrossRefGoogle Scholar
  131. Stacey MG, Hicks SN, von Arnim AG (1999) Discrete domains mediate the light-responsive nuclear and cytoplasmic localization of Arabidopsis COP1. Plant Cell 11:349–363PubMedGoogle Scholar
  132. Stacey MG, Kopp OR, Kim TH, von Arnim AG (2000) Modular domain structure of Arabidopsis COP1. Reconstitution of activity by fragment complementation and mutational analysis of a nuclear localization signal in plants. Plant Physiol 124:979–989PubMedCrossRefGoogle Scholar
  133. Terzaghi WB, Cashmore AR (1995a) Light-regulated transcription. Annu Rev Plant Physiol Plant Mol Biol 46:445–474CrossRefGoogle Scholar
  134. Terzaghi WB, Cashmore AR (1995b) Seeing the light in plant development. Curr Biol 5:466–468PubMedCrossRefGoogle Scholar
  135. Terzaghi WB, Bertekap RL, Cashmore AR (1997) Intracellular localization of GBF proteins and blue light-induced import of GBF2 fusion proteins into the nucleus of cultured Arabidopsis and soybean cells. Plant J 11:967–982PubMedCrossRefGoogle Scholar
  136. Unseld S, Höhnle M, Ringel M, Frischmuth T (2001) Subcellular targeting of the coat protein of African cassava mosaic geminivirus. Virology (in press)Google Scholar
  137. Van den Ackerveken G, Marois E, Bonas U (1996) Recognition of the bacterial avirulence protein AvrBs3 occurs inside the host plant cell. Cell 87:1307–1316PubMedCrossRefGoogle Scholar
  138. Van der Krol AR, Chua NH (1991) The basic domain of plant B-ZIP proteins facilitates import of a reporter protein into plant nuclei. Plant Cell 3:667–675PubMedGoogle Scholar
  139. Varagona MJ, Raikhel NV (1994) The basic domain in the bZIP regulatory protein Opaque2 serves two independent functions: DNA binding and nuclear localization. Plant J 5:207–214PubMedCrossRefGoogle Scholar
  140. Varagona MJ, Schmidt RJ, Raikhel NV (1991) Monocot regulatory protein Opaque-2 is localized in the nucleus of maize endosperm and transformed tobacco plants. Plant Cell 3:105–113PubMedGoogle Scholar
  141. Varagona MJ, Schmidt RJ, Raikhel NV (1992) Nuclear localization signal(s) required for nuclear targeting of the maize regulatory protein Opaque-2. Plant Cell 4:1213–1227PubMedGoogle Scholar
  142. Von Arnim AG, Deng XW (1994) Light inactivation of Arabidopsis photomorphogenic repressor COP1 involves a cell-specific regulation of its nucleocytoplasmic partitioning. Cell 79:1035–1045CrossRefGoogle Scholar
  143. Von Arnim AG, Osterlund MT, Kwok SF, Deng XW (1997) Genetic and developmental control of nuclear accumulation of COP1, a repressor of photomorphogenesis in Arabidopsis. Plant Physiol 114:779–788CrossRefGoogle Scholar
  144. Wang HY, Kang DM, Deng XW, Wei N (1999) Evidence for functional conservation of a mammalian homologue at the light-responsive plant protein COP1. Curr Biol 9:711–714PubMedCrossRefGoogle Scholar
  145. Weisshaar B, Armstrong GA, Block A, Silva O, Hahlbrock K (1991) Light-inducible and constitutively expressed DNA-binding proteins recognizing a plant promoter element with functional relevance in light responsiveness. EMBO J 10:1777–1786PubMedGoogle Scholar
  146. Wellmer F, Kircher S, Rügner A, Frohnmeyer H, Schäfer E, Harter K (1999) Phosphorylation of the parsley bZIP transcription factor CPRF2 is regulated by light. J Biol Chem 274:29476–29482PubMedCrossRefGoogle Scholar
  147. Wengelnik K, Bonas U (1996) HrpXv, an AraC-type regulator, activates expression of five out of six loci in the hrp cluster of Xanthomonas campestris pv. vesicatoria. J Bacteriol 178:3462–3469PubMedGoogle Scholar
  148. Wengelnik K, Van den Ackerveken, Bonas U (1996) HrpG, a key hrp regulatory protein of Xanthomonas campestris pv. vesicatoria is homologous to two-component response regulators. Mol Plant-Microbe Interact 9:704–712PubMedCrossRefGoogle Scholar
  149. Wengelnik K, Rossier O, Bonas U (1999) Mutations in the regulatory gene hrpG of Xanthomonas campestris pv. vesicatoria result in constitutive expression of all hrp genes. J Bacteriol 181:6828–6831PubMedGoogle Scholar
  150. White FF, Yang B, Johnson LB (2000) Prospects for understanding avirulence gene function. Curr Opin Plant Biol 3:291–298PubMedCrossRefGoogle Scholar
  151. Whitelam GC, Halliday KJ (1999) Photomorphogenesis: phytochrome takes a partner! Curr Biol 9:R225–R227PubMedCrossRefGoogle Scholar
  152. Woo Y-M, Itaya A, Owens RA, Tang L, Hammond R, Chou H-C, Lai MMC, Ding B (1999) Characterization of nuclear import of potato spindle tuber viroid RNA in permeabi-lized protoplasts. Plant J 17:627–635CrossRefGoogle Scholar
  153. Wright EA, Heckel T, Groenendijk J, Davies JW, Boulton MI (1997) Splicing features in maize streak virus virion- and complementary-sense gene expression. Plant J 12:1285–1297PubMedCrossRefGoogle Scholar
  154. Wu C (1995) Heat shock transcription factors: structure and regulation. Annu Rev Cell Dev Biol 11:441–469PubMedCrossRefGoogle Scholar
  155. Yamaguchi R, Nakamura M, Mochizuki N, Kay SA, Nagatani A (1999) Light-dependent translocation of a phytochrome B-GFP fusion protein to the nucleus in transgenic Arabidopsis. J Cell Biol 145:437–445PubMedCrossRefGoogle Scholar
  156. Yang B, Zhu W, Johnson LB, White FF (2000) The virulence factor AvrXa7 of Xanthomonas oryzae pv. oryzae is a type III secretion pathway-dependent nuclear-localized double-stranded DNA-binding protein. Proc Natl Acad Sci USA 97:9807–9812PubMedCrossRefGoogle Scholar
  157. Zentgraf U, Hemleben V (1996): Molecular cell biology: signal transduction in plants. Prog Bot 57:218–234Google Scholar
  158. Zentgraf U, Velasco R, Hemleben V (1998) Molecular cell biology: different transcriptional activities in the nucleus. Prog Bot 59:131–168CrossRefGoogle Scholar
  159. Zupan J, Muth TR, Draper O, Zambryski P (2000) The transfer of DNA from Agrobacterium tumefaciens into plants: a feast of fundamental insight. Plant J 23:11–28PubMedCrossRefGoogle Scholar
  160. Zupan JR, Citovsky V, Zambryski P (1996) Agrobacterium VirE2 protein mediates nuclear uptake of single-stranded DNA in plant cells. Proc Natl Acad Sci USA 93:2392–2397PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2002

Authors and Affiliations

  • Vera Hemleben
    • 1
  • Katrin Hinderhofer
    • 1
  • Ulrike Zentgraf
    • 1
  1. 1.ZMBP (Zentrum für Molekularbiolobgie der Pflanzen) Allgemeine GenetikTübingenGermany

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