Abstract
The influence of gadolinium chloride (GdCl3)-pre-treatment on the gene expression of pro-inflammatory (IL-1β, IL-6, TNF-α) and anti inflammatory genes (IL-1β receptor antagonist (IL-1RA), IL-10) in Kupffer cells derived from rats with an LPS induced septic shock was investigated using RT-PCR technique. Kupffer cells were harvested 1 h, 6 h, 12 h after LPS injection and a substantial reduction of IL-6, IL-10, and IL-1β, and a slightly decreased TNF-α as well as an approximately equal IL-1RA gene expression was observed at most time points compared to NaCl pre-treated control animals. These results indicate that GdCl3-blockade of Kupffer cells might not be suitable to treat a hyperinflammation since the expression of anti inflammatory acting genes was also depressed.
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Literatur
Marzi I, Bauer M, Secchi A, Bahrami S, Redi H, Schlag G (1995) Effect of anti-tumor necrosis factor alpha on leukocyte adhesion in the liver after hemorrhagic shock: an intravital microscopic study in the rat. Shock 3: 27–33
Vollmar B, Siegmund S, Richter S, Menger MD (1999) Microvascular consequences of Kupffer cell modulation in rat liver fibrogenesis. J Pathol 189: 85–91
Kawada N, Tran-Thi TA, Klein H, Decker K (1993) The contractil hepatic stellate (Ito) cells stimulated with vasoactive substances. Possible involvement of endothelin-1 and nitric oxide in the regulation of sinusoidal tonus. Eur J Biochem 213: 815–823
Koo DJ, Chaudry IH, Wang P (1999) Kupffer cells are responsible for producing inflammatory cytokines and hepatocellular dysfunction during early sepsis. J Surg Res 83: 151–157
Rai RM, Zhang JX, Clemens MG, Diehl AM (1996) Gadolinium chloride alters the acinar distribution of phagocytosis and balance between pro- and anti-inflammatory cytokines. Shock 6: 243 - 247
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Henrich, D., Herzog, C., Marzi, I. (2002). Gadoliniumchlorid erniedrigt die Genexpression von IL-1beta, IL-6 und IL-10 aber nicht von TNF-alpha und IL-1-Rezeptorantagonist in Kupfferzellen bei septischen Schock in vivo . In: Chirurgisches Forum 2002. Deutsche Gesellschaft für Chirurgie, vol 31. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56158-0_95
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DOI: https://doi.org/10.1007/978-3-642-56158-0_95
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-43300-2
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