Abstract
Recombinant hepatic stimulator substance (rHSS) was expressed in E. coli by using a bacterial expression vector, which fused a 6 histidin-tag at the N-terminus of rHSS. Protein isolation and purification was performed by Ni2+chromatography. rHSS was found as a homodimer under non-reducing conditions. Additionally endogenous HSS was enriched from weanling rat livers and purified by ion exchange chromatography. Polyclonal antibodies against HSS N- and C-terminus were produced. In Western blots antibodies detected rHSS as monomer as well as homodimer from procaryontic and eucaryontic expression systems. To evaluate the influence of rHSS on proliferation potential in liver cells 3H-Thymidin, and BrdU assays were established. Supplementation of culture media with rHSS in combination with EGF, revealed an increase in proliferation of Hep G2 cells and primary human hepatocytes.
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© 2002 Springer-Verlag Berlin Heidelberg
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Thasler, W.E., Weiss, T.S., Letschert, K.C., Hellerbrand, C., An, W., Jauch, KW. (2002). Einfluss von Hepatic Stimulator Substance (HSS) auf das Proliferationspotential von humanen Leberzellen. In: Chirurgisches Forum 2002. Deutsche Gesellschaft für Chirurgie, vol 31. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56158-0_61
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DOI: https://doi.org/10.1007/978-3-642-56158-0_61
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-43300-2
Online ISBN: 978-3-642-56158-0
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