Abstract
Recombinant hepatic stimulator substance (rHSS) was expressed in E. coli by using a bacterial expression vector, which fused a 6 histidin-tag at the N-terminus of rHSS. Protein isolation and purification was performed by Ni2+chromatography. rHSS was found as a homodimer under non-reducing conditions. Additionally endogenous HSS was enriched from weanling rat livers and purified by ion exchange chromatography. Polyclonal antibodies against HSS N- and C-terminus were produced. In Western blots antibodies detected rHSS as monomer as well as homodimer from procaryontic and eucaryontic expression systems. To evaluate the influence of rHSS on proliferation potential in liver cells 3H-Thymidin, and BrdU assays were established. Supplementation of culture media with rHSS in combination with EGF, revealed an increase in proliferation of Hep G2 cells and primary human hepatocytes.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Literatur
Fleig WE, Hoss G (1989) Partial purification of rat hepatic stimulator substance and characterization of its action on hepatoma cells and normal hepatocytes. Hepatology 9: 240 - 248
Francavilla A, Hagiya M, Porter KA, Polimeno L, Ihara I, Starzl TE (1994) Augmenter of liver regeneration: its place in the universe of hepatic growth factors. Hepatology 20: 747 - 757
Hagiya M, Francavilla A, Polimeno L, Ihara I, Sakai H, Seki T, Shimonishi M, Porter KA, Starzl TE (1994) Cloning and sequence analysis of the rat augmenter of liver regeneration (ALR) gene:expression of biologically active recombinant ALR and demonstration of tissue distribution. Proc Natl Acad Sci USA 91: 8142–8146
Li Y, Li M, Xing G, Hu Z, Wang Q, Dong C, Wie H, Fan G, Chen J, Yang X, Zhao S, Chen H, Guan K, Wu C, Zhang C, He F (2000) Stimulation of the mitogen-activated protein kinase cascade and tyrosine phosphorylation of the epidermal growth factor receptor by hepatopoietin. J Biol Chem 275: 37443–37447
Wang G, Yang X, Zhang Y, Wang Q, Chen H, Wie H, Xing G, Xie L, Hu Z, Zhang C, Fang D, Wu C, He F (1999) Identification and characterization of receptor for mammalian hepatopoietin that is homologous to yeast ERV1. J Biol Chem 274: 11469–11472
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 2002 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Thasler, W.E., Weiss, T.S., Letschert, K.C., Hellerbrand, C., An, W., Jauch, KW. (2002). Einfluss von Hepatic Stimulator Substance (HSS) auf das Proliferationspotential von humanen Leberzellen. In: Chirurgisches Forum 2002. Deutsche Gesellschaft für Chirurgie, vol 31. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56158-0_61
Download citation
DOI: https://doi.org/10.1007/978-3-642-56158-0_61
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-43300-2
Online ISBN: 978-3-642-56158-0
eBook Packages: Springer Book Archive