Abstract
Experimental models of secretory hyperstimulation are used frequently to study the pathophysiology of acute pancreatitis. The cholecystokinin (CCK) analogue ceruletide (Takus®) which is also applied in human medicine is one of the most popular secretagogues. Takus® contains thiomalate (mercaptosuccinat) as a preservative. Thiomalate is known as an inhibitor of the antioxidant enzyme glutathione peroxidase. We tested the hypothesis that the results of secretory stimulation of the pancreas by Takus® may be influenced by the preservative. Acini were isolated from rat pancreas and exposed to the secretagogues ceruletide (Takus®), ceruletide free of preservative (lyophilised Takus®), or cholecystokinin octapeptide (CCK-8) as well as to the preservative alone. Amylase secretion by the acini was measured after 30 min and 60 min of exposure to different concentrations of the secretagogues (10-6 M - 10-11 M). All three secretagogues stimulated amylase secretion in a concentration-dependent manner with a submaximal (10-11 M), an optimal (10-10 M) and an inhibitory (10-9 M - 10-6 M) range. At optimally stimulating concentration, lyophilised Takus® was the most powerful secretagogue followed by CCK-8 and Takus®. Thiomalate at 8 nM - 8 mM was a weak, but at > 80 mM a powerful inhibitor of amylase secretion. Thus, lyophilised Takus® or CCK-8 should be preferentially used vs. Takus® in studies investigating pancreatic secretion. The results may also have relevance to animal models that use supraphysiologic doses of Takus® to induce mild acute pancreatitis since Takus® may provoke oxidative stress independent of pancreatitis itself via inhibition of the antioxidant enzyme glutathione oxidase by the preservative thiomalate. However, this latter suggestion has to be proven by a subsequent investigation.
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Literatur
Michiels C, Remacle J (1988) Use of the inhibition of enzymatic antioxidant Systems in order to evaluate their physiological importance. Eur J Biochem 177: 435–441
Williams JA, Korc M, Dormer RL (1978) Action of secretagogues on a new preparation of functionally intact, isolated pancreatic acini. Am J Physiol 235: E517–E524
Schulz HU, Niederau C, Klonowski-Stumpe H, Halangk W, Lüthen R, Lippert H (1999) Oxidative stress in acute pancreatitis. Hepato-Gastroenterology 46: 2736 - 2750
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© 2002 Springer-Verlag Berlin Heidelberg
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Schulz, HU. et al. (2002). Einfluss unterschiedlicher Cholezystokinin-Analoga auf die Amylasesekretion isolierter Rattenpankreas-Azini. In: Chirurgisches Forum 2002. Deutsche Gesellschaft für Chirurgie, vol 31. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56158-0_55
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DOI: https://doi.org/10.1007/978-3-642-56158-0_55
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-43300-2
Online ISBN: 978-3-642-56158-0
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