Inhibition des Wachstums humaner Fibroblasten durch resterilisierte Kunststoffnetze aus Polypropylen
Introduction: The influence of resterilized polypropylen meshes on proliferation and apoptosis of human fibroblasts was tested in an in vitro experiment. Methods: Polypropylen meshes (Prolene®) were cut into square sheets of 2×2 cm and resterilized (steam autoclave, 121°C, 20 min) according to the manufacturer‘s instructions (Ethicon, Norderstedt). Then human fibroblasts, seeded into six-well culture dishes in a density of 3×104 cells/well, were incubated with mesh pieces over a period of 6,12,18,24,30, 36,42 and 48 hours. Preparations of fibroblasts with non-resterilized meshes and without meshes served as controls. Proliferation index and apoptotic index were estimated by flow cytometry after cell staining with a FITC-conjugated antibody against the Ki-67 antigen or with FITC-conjugated Annexin-V and propidium jodide, respectively. Results: After 48 h a significant reduction of the proliferation index from 86% to 42% was found in fibroblasts incubated with resterilized meshes in contrast to controls (non-resterilized meshes: from 86% to 75%; without meshes: from 86% to 80%). Additionally, the apoptotic index increased significantly from 2% to 48% after 48 h incubation with resterilized meshes, whereas this increase was only slight in the controls (non-sterilized meshes: from 2% to 19%; without meshes: from 2% to 10%). Conclusion: We found a significant growth inhibition of resterilized meshes on human fibroblasts in vitro, demonstrated by a reduced proliferative activity and an increased apoptotic index. This may be caused by toxic substances released from meshes during resterilization, which have a negative influence on cell’s growth. For this reason resterilization should not be recommended.
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- 1.Duchrow M, Windhövel U, Bethge T, Schwandner O, Markert U, Bruch HP, Broll R. (2000) Kunststoffnetze aus Polypropylen beeinflussen das Wachstum humaner Zellen in vitro - Eine experimentelle Studie. Chirurg 73: 154– 160Google Scholar