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Intravitalmikroskopische und pharmakokinetische Untersuchung zur Anreicherung von 5-FU-PEG-Liposomen sowie dynamisches MRT-Monitoring des Langzeitverlaufs an CC-531 lebertumortragenden WAG-Ratten

  • Uwe Pohlen
  • R. Rezska
  • H. J. Buhr
  • B. Hotz
  • T. Foitzik
  • G. Berger
Conference paper
Part of the Deutsche Gesellschaft für Chirurgie book series (DTGESCHIR, volume 31)

Abstract

Controversy still exists for therapy of inoperable colorectal liver metastases. Regional therapy has been considered a promising approach because several clinical studies demonstrated prolonged survival. The aim of this study was to investigate tumor targeting of the most frequently used cytostatic 5-fluouracil by modifying systemic and regional therapy by liposome encapsulation. Our results demonstrate that encapsulation of 5-FU in SUV-PEG liposomes increased tumor concentration by the factor 25 fold with systemic and factor 85 with regional therapy. Reduction of blood flow by starch microspheres (SMS) during regional chemotherapy with liposome-encapsuled 5-FU resulted in an additional 4000 fold increase in 5-FU tumor concentration.

Long-term results showed that 5FU-PEG liposomes + SMS led to a 50% tumor recurrence 2 weeks after therapy, while there was a 10- to 19-fold tumor increase in the i.v. groups.

These results are substantiated by intravital microscopy which revealed the highest fluorescence in tumor tissue in the group receiving 5FU-PEG liposomes/starch microspheres.

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Literatur

  1. 1.
    Berger G, Pohlen U, Reszka R, Lippmann M, Päuser S, Buhr HJ (1996) Pharmakokinetik von liposomal verkapselten Carboplatin. Vergleich verschiedener lokoregionärer Anwendungen. Eine tierexperimentelle Studie am VX-2 Lebertumor. Langenbecks Arch Chir Suppl I: 533 – 536Google Scholar
  2. 2.
    Päuser S, Wagner S, Lippmann M, Pohlen U, Reszka R, Wolf KJ, Berger G (1996) Evaluation of Efficient Chemoembolisation by Magnetic Resonance Imaging Therapy Monitoring: An Experimental Study on the VX-2 Tumor in rabbit liver. Cancer Res 56: 1863 – 1867PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2002

Authors and Affiliations

  • Uwe Pohlen
    • 1
    • 3
  • R. Rezska
    • 2
  • H. J. Buhr
    • 1
  • B. Hotz
    • 1
  • T. Foitzik
    • 1
  • G. Berger
    • 1
  1. 1.Chirurgische Klinik I, Universitätsklinikum Benjamin FranklinFreie Universität BerlinGermany
  2. 2.Institut für MolekularbiologieMax Delbrück CentrumBerlin/BuchGermany
  3. 3.Chirurgische Klinik IUniversitätsklinikum Benjamin FranklinBerlinGermany

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