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Cox-2 unabhängige Zielgene selektiver NSAIDs in kolorektalen Karzinomzellen

  • Eva Riede
  • C. Hanski
  • M. Zeitz
  • H. J. Buhr
  • B. Mann
Conference paper
Part of the Deutsche Gesellschaft für Chirurgie book series (DTGESCHIR, volume 31)

Abstract

Nonsteroidal anti-inflammatory drugs (NSAIDs) are able to inhibit the growth of colorectal cancer cells. This effect could be shown in cyclooxygenase-2+ (COX-2+) as well as in COX-2- cell lines. Therefore other effects than the known COX-2 inhibition must be also responsible for the tumoricidal activity of NSAIDs. To evaluate the involvement of COX-2-independent mechanisms we assessed the effects of Nabumetone and Celecoxib on cell survival, cell cycle and apoptosis in a COX-2+ (HT29) and a COX-2- (DLD1) colorectal carcinoma cell line. Both drugs induced in both cell lines a G1 arrest. The cell death was detectable in both cell lines after Celecoxib but not after Nabumetone treatment. Increased expression of 5 genes on mRNA level was detectable in a cDNA array in both cell lines after treatment. The following genes were upregulated more than two-fold after treatment: GADD153, MIC1/NAG1, VEGF, DTR, PC4 homolog. This data indicate that several genes potentially involved in induction of cell arrest and/or apoptosis are activated by NSAIDs in a COX-2-independent manner. This may explain tumoricidal effects of NSAIDs in COX-2-cell lines.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2002

Authors and Affiliations

  • Eva Riede
    • 1
    • 3
  • C. Hanski
    • 2
  • M. Zeitz
    • 2
  • H. J. Buhr
    • 1
  • B. Mann
    • 1
  1. 1.Chirurgische Klinik IGermany
  2. 2.Gastroenterologische KlinikUniversitätsklinikum Benjamin FranklinBerlinGermany
  3. 3.Abteilung fur Allgemein-, Gefäß- und ThoraxchirurgieUniversitätsklinikum Benjamin FranklinBerlinGermany

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