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Hohe Mutationsfrequenz in der mitochondrialen DNA D-Loop Region bei Adenocarcinomen im Barrett-Ösophagus

  • Paul M. Schneider
  • F. Miyazono
  • R. Metzger
  • U. Warnecke-Eberz
  • P. H. Collet
  • S. E. Baldus
  • H. Schäfer
  • T. Aikou
  • A. H. Hölscher
Conference paper
Part of the Deutsche Gesellschaft für Chirurgie book series (DTGESCHIR, volume 31)

Abstract

Mitochondrial DNA (mtDNA) is known for high mutation rates caused by lack of protective histones, inefficient DNA repair systems, and continuous exposure to mutagenic effects of oxygen radicals.

In the present study, we examined the frequency of mutations in the mtDNA D-loop region in 20 patients with Barrett’s carcinoma and associated Barrett’s epithelium by automated DNA sequencing. Mutations were detected in 8 of 20 (40%) patients in tumor and/or tumor-associated Barrett’s epithelium. There was no association of mtDNA D-loop mutations with histopathological stage of disease (UICC) or tumor grading.

We present the first report on frequent occurrence of mutations in the mtDNA D-loop regions in adenocarcinomas in Barrett’s esophagus. Furthermore, this study strongly supports the hypothesis that oxidative damage is an important mechanism for the induction of adenocarcinoma in Barrett’s esophagus.

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Literatur

  1. 1.
    Taanman JW (1999) The mitochondrial genome: structure, transcpription, translation and replication. Biochim Biophys Acta 1410: 103–123PubMedCrossRefGoogle Scholar
  2. 2.
    Wallace DC (1994) Mitochondrial DNA sequence Variation in human evolution and disease. Proc Natl Acad Sci USA 91: 8739–8746PubMedCrossRefGoogle Scholar
  3. 3.
    Richter C, Park JW, Arnes BN (1988) Normal oxidative damage to mitochondrial and nuclear DNA is extensive. Proc Natl Acad Sci USA 85: 6465 - 6467PubMedCrossRefGoogle Scholar
  4. 4.
    Olyaee M, Sontag S, Salman W, Schnell T, Mobarhan S, Eiznhamer D, Keshavarzian A (1995) Mucosal reactive oxygen species produetion in oesophagitis and Barrett’s oesophagus. Gut 37: 168– 173PubMedCrossRefGoogle Scholar
  5. 5.
    Wilson MR, Polanskey D, Butler J, DiZinno JA, Replogle J, Budowle B (1995) Extraction, PCR amplification and sequencing of mitochondrial DNA from human hair shafts. BioTechniques, 18: 662 -669PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2002

Authors and Affiliations

  • Paul M. Schneider
    • 1
    • 4
  • F. Miyazono
    • 1
    • 3
  • R. Metzger
    • 1
  • U. Warnecke-Eberz
    • 1
  • P. H. Collet
    • 1
  • S. E. Baldus
    • 2
  • H. Schäfer
    • 1
  • T. Aikou
    • 1
    • 3
  • A. H. Hölscher
    • 1
  1. 1.Klinik und Poliklinik für Visceral- und GefässchirurgieGermany
  2. 2.Institut für PathologieUniversität KölnGermany
  3. 3.Chirurgische KlinikUniversität KagoshimaJapan
  4. 4.Klinik und Poliklinik für Visceral-und GefäßchirurgieUniversität zu KölnKölnGermany

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