Integration Site Selection by Lentiviruses: Biology and Possible Control
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Retroviral vectors are popular vehicles for gene transfer, since they neatly integrate a single copy of a new gene into a transduced cell. The viral cDNA that becomes integrated is colinear with the vector genome, yielding a predictable final product. Gene transfer by transfection of naked DNA, in contrast, leads to the formation of scrambled arrays of new sequences that are often unstable and unpredictable in their behavior. Retroviral cDNA integration is sequence nonspecific with respect to the choice of target site; however, this raises a variety of issues surrounding the safety and effectiveness of retroviral vectors. This chapter first covers studies of target site selection by retroviruses, particularly HIV-1, and then the efforts being made to control target site selection in vivo.
KeywordsIntegration Site Ciency Virus Viral cDNA Human Immunode SupT1 Cell
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