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Ratiometric Probes: Design and Applications

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Fluorescence Spectroscopy, Imaging and Probes

Part of the book series: Springer Series on Fluorescence ((SS FLUOR,volume 2))

Abstract

With the aim of substantial improvement of solvatochromic and electrochromic fluorescence probes by coupling their response with an excitedstate reaction, a series of 3-hydroxychromone derivatives have been synthesized. They demonstrate two well-separated emission bands that originate from normal and phototautomer forms due to excited-state intramolecular proton transfer (ESIPT) reaction. In the studies of solvent polarity effect, electrochromism (internal Stark effect) and red edge effect a strong amplification of probe response is achieved by recording of relative intensity ratios of these fluorescence bands instead of their spectral shifts. This result is an illustration of a new principle in design of fluorescence probes, which may allow the achievement of almost perfect on-off switching behavior.

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Authors
  1. A. P. Demchenko
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  2. A. S. Klymchenko
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  3. V. G. Pivovarenko
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  4. S. Ercelen
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Editor information

Editors and Affiliations

  1. Institute of Molecular Biological Sciences, Amsterdam, Vrije Universiteit, De Boelelaan 1087, 1081 HV, Amsterdam, The Netherlands

    Ruud Kraayenhof

  2. MicroSpectroscopy Center, Wageningen University, Dreijenlaan 3, 6703 HA, Wageningen, The Netherlands

    Antonie J. W. G. Visser

  3. Dept. of Molecular Biophysics, University of Utrecht, Princetonplein 1, 3584 CC, Utrecht, The Netherlands

    Hans C. Gerritsen

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© 2002 Springer-Verlag Berlin Heidelberg

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Demchenko, A.P., Klymchenko, A.S., Pivovarenko, V.G., Ercelen, S. (2002). Ratiometric Probes: Design and Applications. In: Kraayenhof, R., Visser, A.J.W.G., Gerritsen, H.C. (eds) Fluorescence Spectroscopy, Imaging and Probes. Springer Series on Fluorescence, vol 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56067-5_5

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  • DOI: https://doi.org/10.1007/978-3-642-56067-5_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-62732-3

  • Online ISBN: 978-3-642-56067-5

  • eBook Packages: Springer Book Archive

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