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Expression of Multicolor Fluorescent Fusion Proteins in Zebrafish Cell Cultures: A Versatile Tool in Cell Biology

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Fluorescence Spectroscopy, Imaging and Probes

Part of the book series: Springer Series on Fluorescence ((SS FLUOR,volume 2))

Abstract

A genetically stable zebrafish cell line was transfected with plasmids encoding fluorescent marker proteins of various colors. The markers for human tubulin, actin, ER, golgi and endosome are located at their correct position in zebrafish cells. We discus the benefits of transfected zebrafish cell lines over mammalian cells in cell biological studies.

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Authors
  1. C. K. D. Breek
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  2. F. Van Iren
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  3. S. E. Wijting
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  4. N. Stuurman
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  5. H. P. Spaink
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Editor information

Editors and Affiliations

  1. Institute of Molecular Biological Sciences, Amsterdam, Vrije Universiteit, De Boelelaan 1087, 1081 HV, Amsterdam, The Netherlands

    Ruud Kraayenhof

  2. MicroSpectroscopy Center, Wageningen University, Dreijenlaan 3, 6703 HA, Wageningen, The Netherlands

    Antonie J. W. G. Visser

  3. Dept. of Molecular Biophysics, University of Utrecht, Princetonplein 1, 3584 CC, Utrecht, The Netherlands

    Hans C. Gerritsen

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© 2002 Springer-Verlag Berlin Heidelberg

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Breek, C.K.D., Van Iren, F., Wijting, S.E., Stuurman, N., Spaink, H.P. (2002). Expression of Multicolor Fluorescent Fusion Proteins in Zebrafish Cell Cultures: A Versatile Tool in Cell Biology. In: Kraayenhof, R., Visser, A.J.W.G., Gerritsen, H.C. (eds) Fluorescence Spectroscopy, Imaging and Probes. Springer Series on Fluorescence, vol 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56067-5_23

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  • DOI: https://doi.org/10.1007/978-3-642-56067-5_23

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-62732-3

  • Online ISBN: 978-3-642-56067-5

  • eBook Packages: Springer Book Archive

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