Abstract
The use of stable isotopes at natural abundance levels has brought a new dimension to our understanding of plant ecology. In the past two decades there have been remarkable advances in the theoretical understanding of discrimination processes, as well as technical developments in mass spectrometry, leading to an exponential growth in applications for natural systems. Stable isotopes provide plant ecologists with a quantitative and integrative framework for chemical, biological and ecological transformations. The range of stable isotopes currently routinely analysed for ecological applications and their natural abundances are shown in Table 1. Analyses of the relative natural abundances of stable isotopes of carbon (13C/12C), oxygen (18O/16O), nitrogen (15N/14N) and deuterium (D/H) have been used across a wide range of scales, from cellular to community and ecosystem level, contributing much to our understanding of the interactions between biosphere, pedosphere and atmosphere.
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References
Acevedo E (1993) Potential of carbon isotope discrimination as a selection criterion in barley breeding. In: Ehleringer JR, Hall AE, Farquhar GD (eds) Stable isotopes and plant carbon-water relations. Academic Press, San Diego, pp 399–417
Adams MA, Grierson PF (2001) Stable isotopes and natural abundance in terrestrial plant ecology and ecophysioloy: an update. Plant Biol 3:299–310
Anderson JE, Williams J, Kriedemann PE, Austin MP, Farquhar GD (1996) Correlations between carbon isotopic discrimination and climate of native habitats for diverse eucalypt taxa growing in a common garden. Aust J Plant Physiol 23:311–320
Antos JA, Allen GA (1994) Biomass allocation among reproductive structures in the dioecious shrub Oemleria cerasiformis — a functional interpretation. J Ecol 82:21–29
Araus JL, Reynolds MP, Acevedo E (1993) Leaf posture, Grain yield growth, Leaf structure and carbon isotope discrimination. Crop Sci 33:1273–1279
Barbour MM, Schurr, Henry BK, Wong SC, Farquhar GD (2000) Variation in the oxygen isotope ratio of phloem sap of sucrose from castor bean: evidence in support of the Péclet effect. Plant Physiol 123:671–679
Beardall J, Griffiths H, Raven J (1982) Carbon isotope discrimination and the CO2 accumulating mechanism in Chlorella emersonii. J Exp Bot 33:729–737
Boddey RM, Peoples MB, Palmer B (2000) Use of the 15N natural abundance technique to quantify biological nitrogen fixation by woody perennials. Nutr Cycling Agroecosyst 57:235–270
Borland AM, Griffiths H, Broadmeadow MSJ, Fordham M, Maxwell C (1994) Carbon isotope composition of biochemical fractions and the regulation of carbon balance in leaves of the C3-CAM intermediate Clusia minor L. growing in Trinidad. Plant Physiol 106:493–501
Broadmeadow MSJ, Griffiths H (1993) Carbon isotope discrimination and the coupling of CO2 fluxes within forest canopies. In: Ehleringer JR, Hall AE, Farquhar GD (eds) Stable isotopes and plant carbon-water relations. Academic Press, San Diego, pp 109–129
Broadmeadow MSJ, Griffiths H, Maxwell, Borland AM (1992) The carbon isotope ratio of plant organic material reflects temporal and spatial variations in CO2 within tropical forest formations in Trinidad. Oecologia 89:435–441
Brugnoli E, Björkman O (1992) Growth of cotton under continuous salinity stress: influence on allocation pattern, Stomatal and non-stomatal components of photosynthesis and dissipation of excess light energy. Planta 187:335–347
Brugnoli E, Farquhar GD (2000) Photosynthetic fractionation of carbon isotopes. In: Leegood RG Sharkey TD, Caenumerer (eds) Photosynthesis physiology and metabolism. Kluwer, Dordrecht, pp 399–434
Brugnoli E, Hubick K, von Caemmerer S, Wong S, Farquhar G (1988) Correlation between the carbon isotope discrimination in leaf starch and sugars of C3 plants and the ratio of intercellular and atmospheric partial pressure of carbon dioxide. Plant Physiol 88:1418–1424
Brugnoli E, Scartazza A, Lauteri M, Montverdi MC, Máguas C (1998) Carbon isotope discrimination in structural and non-structural carbohydrates in relation to productivity and adaptation to unfavourable conditions. In: Griffiths H (ed) Stable isotopes: integration of biological, Ecological and geochemical processes. Bios Scientific, Oxford, pp 133–146
Buchmann N, Brooks R, Flanagan L, Ehleringer J (1998) carbon isotope discrimination of terrestrial ecosystems. In: Griffiths H (ed) Stable isotopes: integration of biological, ecological and geochemical processes. Bios Scientific, Oxford, pp 203–218
Caldwell MM, Dawson TE, Richards JH (1998) Hydraulic lift: consequences for water efflux from the roots of plants. Oecologia 113:151–161
Ciais P, Meijer HAJ (1998) The 18O/16O isotope ratio of atmospheric CO2 and its role in global carbon cycle research. In: Griffiths H (ed) Stable isotopes: integration of biological, ecological and geochemical processes. Bios Scientific, Oxford, pp 409–438
Ciais P, Tans PP, Trolier M, White JWC, Francey RJ (1995) A large northern hemisphere terrestrial CO2 sink indicated by the 13C/12C ratio of atmospheric CO2. Science 269:1098–1101
Comstock J, Ehleringer J (1993) Stomatal response to humidity in common bean (Phaseoulus vulgaris): implications for maximum transpiration rate, Water-use efficiency and productivity. Aust J Plant Physiol 20:669–691
Condon AG, Richards RA, Farquhar GD (1987) Carbon isotope discrimination is positively correlated with grain yield and dry matter production in field-grown wheat. Crop Sci 27:996–1001
Correia O, Diaz-Barradas MC (2000) Ecophysiological differences between male and female plants of Pistacia lentiscus L. Plant Ecol 149:131–142
Correia OA, Martins AC, Catarino FM (1992) Comparative phenology and seasonal foliar nitrogen variation in Mediterranean species of Portugal. Ecol Mediterr 18:7–18
Cowan IR, Lange O, Green A (1992) Carbon-dioxide exchange in lichens: determination of transport and carboxylation characteristics. Planta 18:282–294
Cox PA (1981) Niche partitioning between sexes of dioecious plants. Am Nat 117:295–307
Craig H (1954) Carbon 13 in plants and the relationship between carbon 13 and carbon 14 variations in nature. J Geol 62:115–149
Dawson TE (1993a) Water sources of plants as determined from xylem-water isotopic composition: perspectives on plant competition, Distribution, And water relations. In: Ehleringer J, Hall A, Farquhar G (eds) Stable isotopes and plant carbon-water relations. Academic Press, London, pp 465–496
Dawson TE (1993b) Hydraulic lift and water use by plants: implications for performance, water balance and plant-plant interactions. Oecologia 95:565–574
Dawson TE (1998) Fog in California redwood forest: ecosystem inputs and use by plants. Oecologia 117:476–485
Dawson TE, Bliss LC (1989) Patterns of water use and the tissue water relations in the dioecious shrub Salix artica: the physiological basis for habitat partitioning between the sexes. Oecologia 79:332–343
Dawson TE, Ehleringer JR (1991) Streamside trees that do not use stream water. Nature 350:335–337
Dawson TE, Ehleringer JR (1993) Gender-specific physiology, carbon isotope discrimination and habitat distribution in box elder, Elder negundo. Ecology 74:798–815
Deléens-Provent E, Schwebel-Gdugué N (1987) Demonstration of autrophic state and establishment of full tropical C4 pathway in maize seedlings by photosynthate carbon isotope composition. Plant Physiol Biochem 25:567–572
DeLucia EH, Schlesinger WEI (1991) Resource-use efficiency and drought tolerance in adjacent great basin and Sierran plants. Ecology 72:51–58
Diaz-Barradas MC, Correia O (1999) Sexual dimorphism, Sex ratio and spatial distribution of male and female shrubs in the dioecious species Pistacia lentiscus L. Fol Geobot 34:163–174
Duranceau M, Ghashghaie J, Brugnoli E (2001) Carbon isotope discrimination during photosynthesis and dark respiration in intact leaves of Nicotiana sylvestris: comparison between wild type and mitochondrial mutant plants. Aust J Plant Physiol 28:65–71
Ehleringer JR, Cerling TE (1995) Atmospheric CO2 and the ratio of intercellular to ambient CO2 levels in plants. Tree Physiol 15:562–566
Ehleringer JR, Cook CS (1998) Carbon and oxygen isotopes ratios of ecosystem respiration along an Oregon conifer transect: preliminary observations based on small-flask sampling. Tree Physiol 18:513–519
Ehleringer JR, Dawson TE (1992) Water uptake by plants: perspectives from stable isotopes. Plant Cell Environ 15:1073–1082
Ehleringer JR, Philips SL, Schuster WEF, Sandquist DR (1991) Differential utilisation of summer rains by desert plants, Implications for competition and climate change. Oecologia 88:430–434
Ehleringer JR, Philips SL, Comstock JP (1992) Seasonal variation in carbon isotopic composition of desert plants. Funct Ecol 6:396–404
Ehleringer JR, Schwinning S, Gebauer RLE (1999) Water use in arid land ecosystems. In: Press MC, Barker MG (eds) Physiological plant ecology. Blackwell, Oxford
Evans JR, Sharkey TD, Berry JA, Farquhar GD (1986) Carbon isotope discrimination measured concurrently with gas exchange to investigate CO2 diffusion in leaves of higher plants. Aust J Plant Physiol 13:281–292
Evans JR, von Caemmerer S, Setchell BA, Hudson GS (1994) The relationship between CO2 transfer conductance and leaf anatomy in transgenic tobacco with reduced content of Rubisco. Aust J Plant Physiol 21:475–495
Evans RD (2001) Physiological mechanisms influencing plant nitrogen isotope composition. Tree 6:121–126
Evans RD, Belnap J (1999) Long term consequences of disturbance on nitrogen dynamics in an arid ecosystem. Ecology 80:150–160
Evans JR, von Caemmerer S (1996) Carbon dioxide diffusion within leaves. Plant Physiol 110:339–346
Evans RD, Bloom AJ, Sukrapanna SS, Ehleringer JR (1996) Nitrogen isotope composition of tomato (Lycopersicon esculentum Mill. cv. T-5) grown under ammonium of nitrate nutrition. Plant Cell Environ 19:1317–1323
Farquhar GD, Lloyd J (1993) Carbon and oxygen isotope effects in the exchange of carbon dioxide between terrestrial plants and the atmosphere. In: Ehleringer J, Hall A, Farquhar G (eds) Stable isotopes and plant carbon-water relations. Academic Press, London, pp 47–70
Farquhar GD, Richards RA (1984) Isotopic composition of plant carbon correlates with water-use-efficiency of wheat genotypes. Aust J Plant Physiol 11:359–552
Farquhar GD, O’Leary MH, Berry JA (1982) On the relationship between carbon isotope discrimination and the intercellular carbon dioxide concentration in leaves. Aust J Plant Physiol 9:121–137
Farquhar GD, Ehleringer JR, Hubick KT (1989) Carbon isotope discrimination and photosynthesis. Annu Rev Plant Physiol Plant Mol Biol 40:503–537
Farquhar GD, Lloyd J, Taylor JA, Flanagan LB, Syversten JP, Hubik KT, Et al (1993) Vegetation effects on the isotopic composition of oxygen in atmospheric CO2. Nature 363:439–442
Farquhar GD, Barbour MM, Henry BK (1998) Interpretation of oxygen isotope composition of leaf material. In: Griffths H (ed) Stable isotopes: integration of biological, ecological and geochemical processes. Bios Scientific, Oxford, pp 27–62
Flanagan LB (1998) Oxygen isotope effects during CO2 exchange: from leaf to ecosystem processes. In: Griffiths H (ed) Stable isotopes: integration of biological, Ecological and geochemical processes. Bios Scientific, Oxford, pp 185–201
Flanagan LB, Ehleringer JR (1991) Stable isotope composition of steam and leaf water: applications to the study of plant water use. Funct Ecol 5:270–277
Flanagan LB, Phillips SL, Ehleringer JR, Loyd J, Farquhar GD (1994) Effect of changes in leaf water oxygen isotopic composition on discrimination against C18O16O during photosynthetic gas exchange. Aust J Plant Physiol 21:221–234
Fontes JC (1980) Environmental isotopes in groundwater hydrology. In: Fritz P, Fontes JC (eds) Handbook of environmental isotope biochemistry. Elsevier, Amsterdam, pp 75–140
Francey RJ, Gifford RM, Sharkey TD, Weir B (1985) Physiological influences on carbon isotope discrimination in huon pine (Lagarostrobos franklinii). Oecologia 66:211–218
Garten CT, Taylor GE (1992) Foliar δ13C within a temperate deciduous forest: spatial, temporal and species sources of variation. Oecologia 90:1–7
Gat JR (1996) Oxygen and hydrogen isotopes in the hydrologic-cycle. Annu Rev Earth Planet Sci 24:225–262
Gebauer R, Ehleringer J (2000) Water and nitrogen uptake patterns following moisture pulses in a cold desert community. Ecology 81:1415–1424
Ghashghaie J, Badek F, Tcherkez G, Deléens (2001) Carbon isotope discrimination during dark respiration in C3 plants. Plant Cell Environ 24:505–515
Gillon JS, Griffiths H (1997) The influence of (photo)respiration on carbon isotope discrimination in plants. Plant Cell Environ 20:1217–1230
Gillon JS, Borland AM, Harwood KG, Roberts A, Broadmeadows MSJ, Griffiths H (1998) Carbon isotope discrimination in terrestrial plants: carboxylations and decarboxylations. In: Griffiths H (ed) Stable isotopes: integration of biological, Ecological and geochemical processes. Bios Scientific, Oxford, pp 111–131
Gleixner G, Danier HJ, Werner RA, Schmidt HL (1993) Correlations between the 13C content of primary and secondary plant products in different cell compartments and that in decomposing basidiomycetes. Plant Physiol 102:1287–1290
Green TGA, Lange OL (1994) Photosynthesis in poikilohydric plants: a comparison of lichens and bryophytes. In: Schulze E, Caldwell M (eds) Ecophysiology of photosynthesis. Ecological studies. Springer, Berlin Heidelberg New York, pp 319–342
Griffiths H (1996) Evaluation and integration of environmental stress using stable isotopes. In: Baker NR (ed) Photosynthesis and the environment. Kluwer, Dordrecht, pp 451–468
Griffiths H, Broadmeadow MSJ, Bordland AM, Hetherington CS (1990) Short-term changes in carbon isotope discrimination identify transitions between C3 and C4 carboxylation during crassulacean acid metabolism. Planta 181:604–610
Griffiths H, Borland A, Gillon J, Harwood K, Maxwell K, Wilson J (1999) Stable isotopes reveal exchanges between soil, plants and the atmosphere. In: Press MC, Scholes JD, Barker MG (eds) Physiological plant ecology. The 39th Symposium of the British Ecological Society, University of York, 7–9 September. Blackwell Science, Oxford, pp 415–441
Hanba YT, Mori S, Lei TT, Koike T, Wada E (1997) Variations in leaf Ô13C along a vertical profile of irradiance in a temperate Japanese forest. Oecologia 110:253–261
Hanba YT, Miyazawa SI, Terashima I (1999) The influence of leaf thickness on the CO2 transfer conductance and leaf stable carbon isotope ratio for some evergreen tree species in Japanese warm-temperate forests. Funct Ecol 13:632–639
Harwood KG, Gillon JS, Griffiths H, Broadmeadow MSJ (1998) Diurnal variation of Δ13CO2, ΔC18O16O and evaporative site enrichment of δH2 18O in Piper aduncum under field conditions in Trinidad. Plant. Cell Environ 21:269–283
Harwood KG, Gillon JS, Roberts A, Griffiths H (1999) Stable isotopes (13C and 18O) in ambient CO2 and water vapour reflect the relationships between sources and sinks for gas exchange within a temperate Quercus petraea canopy. Oecologia 119:109–119
Hobbie EA, Macko SA, Williams M (2000) Correlations between foliar 15N and nitrogen concentrations may indicate plant-mycorrhizal interactions. Oecologia 122:273–283
Högberg P (1997) 15N natural abundance in soil-plant systems. New Phytol 137:179–203
Hubick KT, Gibson A (1993) Diversity in the relationship between carbon isotope discrimination and transpiration efficiency when water is limited. In: Ehleringer JR, Hall AE, Faquhar GD (eds) Stable isotopes and plant carbon-water relations. Academic Press, San Diego, pp 311–325
Hubick KT, Farquhar GD, Gshorter R (1986) Correlation between water-use efficiency and carbon isotope discrimination in diverse peanut (Arachis) germplasm. Aust J Plant Physiol 13:803–816
Hultine KR, Marshall JD (2000) Altitude trends in conifer leaf morphology and stable carbon isotope composition. Oecologia 123:32–40
Johnson DA, Asay KH, Tieszen LL, Ehleringer JR, Jefferson PG (1990) Carbon isotope discrimination: potential in screening cool-season grasses for water-limited environments. Crop Sci 30:338–343
Körner CH (1989) The nutritional status of plants from high altitudes: a world-wide comparison. Oecologia 68:379–391
Körner CH, Farquahar GD, Roksandic Z (1988) A global survey of carbon isotope discrimination in plants from high altitude. Oecologia 74:623–632
Körner CH, Farquahar GD, Wong SC (1991) Carbon isotope discrimination by plants follows latitudinal and altitudinal trends. Oecologia 88:30–40
Kohl DH, Shearer GB, Commoner B (1971) Fertilizer nitrogen: contribution to nitrate in surface water in a corn belt watershed. Science 174:1331–1334
Lajha K, Getz J (1993) Photosynthesis and water use efficiency in pinyon-juniper communities along an elevation gradient in northern New Mexico. Oecologia 94:95–101
Lange OL, Green TGA (1996) High thallus water content severely limits photosynthesis carbon gain of central Europe epilithic lichen under natural conditions. Oecologia 198:13–20
Lange OL, Ziegler H (1986) Different limiting processes of photosynthesis in lichens. In: Marcelle R, Clitjers H, van Poucke M (eds) Biological control of photosynthesis. Martinus Nijhoff, Dordrecht, pp 147–161
Lange OL, Green TGA, Ziegler H (1988) Water status related photosynthesis and carbon isotope discrimination in species of the lichen genus Pseudocyphellaria with green and blue green photobionts and in photosymbiodermes. Oecologia 75:494–501
Lauteri M, Scartazza A, Guido MC, Brugnoli E (1997) Genetic variation in photosynthetic capacity, carbon isotope discrimination and mesophyll conductance in provenances of Castanea sativa adapted to different environments. Funct Ecol 11:675–683
Leavitt SW, Long A (1982) Evidence for13C/12C fractionation between tree leaves and wood. Nature 298:742–744
Le Roux XI, Bariac T, Sinoquet H, Genty B, Piel C, Maritti A, Girardin C, Richard P (2001) Spatial distribution of leaf-use efficiency and carbon isotope discrimination within an isolated tree crown. Plant Cell Environ 24:1021–1032
Li C, Berninger F, Koskela J, Sonninen E (2000) Drought responses of Eucalyptus michroteca provenance dependent seasonality of rainfall in their place of origin. Aust J Plant Physiol 27:231–238
Lin G, Ehleringer JR (1997) Carbon isotopic fractionation does not occur during dark respiration in C3 and C4 plants. Plant Physiol 114:391–394
Lloyd J, Farquhar GD (1994) 13C discrimination during CO2 assimilation by the terrestrial biosphere. Oecologia 99:201–215
Madhavan S, Treichel I, O’Leary MH (1991) Effects of relative humidity on carbon isotope fractionation in plants. Bot Acta 104:292–294
Máguas C, Brugnoli E (1996) Spatial variation in carbon isotope discrimination across the thalli of several lichen species. Plant Cell Environ 19:437–446
Máguas C, Griffiths H, Ehleringer JR, Serodio J (1993) Characterisation of photobiont associates in lichens using carbon isotopic discrimination techniques. In: Ehleringer J, Hall AE, Farquhar GD (eds) Stable isotopes and plant carbon-water relations. Academic Press, Oxford, pp 201–212
Máguas C, Griffiths H, Broadmeadow MSJ (1995) Gas exchange and carbon isotope discrimination in lichen: evidence for interactions between CO2-concentration mechanism and diffusion limitation. Planta 196:95–102
Máguas C, Valladares F, Brugnoli E (1997) Effects of thallus size on morphology and physiology of foliose lichens: new findings with a new approach. Symbiosis 23:149–164
Marshall JD, Zhang J (1994) Carbon isotope discrimination and water use efficiency in native plants of the northern-central Rockies. Ecology 75:1887–1895
Matzner SL, Rice KJ, Richards JH (2001) Factors affecting the relationship between carbon isotope discrimination and transpiration efficiency in bleu-oak (Quercus douglasii). Aust J Plant Physiol 28:49–56
Maxwell K, von Caemmerer S, Evans JR (1997) Is a low internal conductance to CO2 diffusion a consequence of succulence in plants with crassulacean acid metabolism? Aust J Plant Physiol 24:777–786
Meier-Augenstein W (1999) Applied gas chromatography coupled to isotope mass spectrometry. J Chromatogr 824:351–371
Meinzer FC, Rundel PW, Goldstein G, Sharifi MR (1992) Carbon isotope composition in relation to leaf gas exchange and environmental conditions in Hawaiian Metrosideros polyrnorpha population. Oecologia 91:305–311
Melzer A, Schmidt HL (1987) Carbon isotope effects on the pyruvate dehydrogenase reaction and their importance for relative carbon-13 depletion in lipids. J Biol Chem 20:8159–8164
Miller JM, Williams RJ, Farquar GD (2001) Carbon isotope discrimination by a sequence of Eucalyptus species along a subcontinental rainfall gradient in Australia. Funct Ecol 15:222–232
Miranda AC, Miranda HS, Lloyd J, Grace J, Francey RJ, Mcintyre JA, Meir P, Riggan P, Lockwood R, Brass J (1997) Fluxes of carbon, Water and energy over Brazilian cerrado: an analysis using eddy covariance and stable isotopes. Plant Cell Environ 20:315–328
Miyazawa SI, Terashima I (2001) Slow development of leaf photosynthesis in an ever-green broad-leaved tree, Castanopsis sieboldii: relationships between leaf anatomical characteristics and photosynthetic rate. Plant Cell Environ 24:279–291
Niinemets U, Kull O, Tenhunen JD (1999) Variability in leaf morphology and chemical composition as a function of canopy light environment in coexisting deciduous trees. Int J Plant Sci 160:837–848
Obeso JR (1997) Cost of reproduction in Ilex aquifolium: effects on tree, branch and leaf levels. J Ecol 85:159–166
O’Leary MH (1981) Carbon isotope fractionation in plants. Phytochemistry 20:553–567
O’Leary MH (1988) Carbon isotopes in photosynthesis. Bioscience 38:325–336
Palmqvist K, Máguas C, Badger MR, Griffiths H (1994) Assimilation, Accumulation and carbon isotope discrimination of inorganic carbon in lichens: further evidence for the operation of a CO2 concentration mechanism in cyanobacterial lichens. Crypt Bot 4:218–226
Panek JA, Waring RH (1995) Carbon isotope variation in Douglas-fir foliage: improving the 13C-climate relationship. Tree Physiol 15:657–663
Panek JA, Waring RH (1997) Stable carbon isotopes as indicators of limitations to forest growth imposed by climate stress. Ecol Appl 7:854–863
Pearcy RW, Pfitsch WA (1994) Influence of sunflecks on the δ13C of Adenocaulon bicolor plants occuring in contrasting understorey microsites. Oecologia 86:457–462
Prasolova NV, Xu ZH, Farquhar GD, Saffigna PG, Dieters MJ (2000) Variations in branchlet δ13C in relation to branchlet nitrogen concentration and growth in 8-year old hoop pine families (Auracaria cunninghamii) in subtropical Australia. Tree Physiol 20:1024–1055
Proctor MCF, Raven JA, Rice SK (1992) Stable carbon isotope discrimination measurements in Sphagnum and other bryophytes: physiological and ecological implications. J Bryol 17:193–202
Retuerto R, Lema BF, Roiloa SR, Obeso J R (2000) Gender, Light and water effects in carbon isotope discrimination, and growth rates in the dioecious tree Ilex aquifolium. Funct Ecol 14:529–537
Rice SK (2000) Variation in carbon isotope discrimination within and among Sphagnum species in a temperate wetland. Oecologia 123:1–8
Rice SK, Giles L (1996) The influence of water content and leaf anatomy on carbon isotope discrimination and photosynthesis in Sphagnum. Plant Cell Environ 19:118–124
Roberts A, Bordland AM, Griffiths H (1997) Discrimination processes and shift in carboxylation during the phases of crassulacean acid metabolism. Plant Physiol 113:1283–1292
Robinson D (2001) δ15N as an integrator of the nitrogen cycle. Trees 16:153:161
Rooney MA (1988) Short-term carbon isotope fractionation by plants. PhD Thesis, University of Wisconsin, Wisconsin, USA
Rossman A, Butzenlechner M, Schmidt H (1991) Evidence for nonstatistical carbon isotope distribution in natural glucose. Plant Physiol 96:609–614
Rundel PW, Stichler W, Zander RH, Ziegler H (1979) Carbon and hydrogen isotopes ratios of bryophytes from arid and humid regions. Oecologia 44:91–94
Saurer M, Allen K, Siegwolf RTW (1997) Correlating δ13C and δ18O in cellulose of trees. Plant Cell Environ 20:1543–1550
Scartazza A, Lauteri M, Guido MC, Brugnoli E (1998) Carbon isotope discrimination in leaf and stem sugars, Water use efficiency and mesophyll conductance during different developmental stages in rice subjected to drought. Aust J Plant Physiol 25:489–498
Schmidt HL, Gleixner G (1998) Carbon isotope effects on key reactions in plant metabolism and 13C-patterns in natural compounds. In: Griffiths H (ed) Stable isotopes: integration of biological, Ecological and geochemical processes. Bios Scientific, Oxford, pp 13–25
Sharkey TD, Berry J (1985) Carbon isotope fractionation of algae as influenced by an inducible CO2 concentrating mechanism. In: Lucas WJ, Berry J (eds) Inorganic carbon uptake by aquatic organisms. American Society of Plant Physiology, Rockville, Maryland, pp 389–402
Shearer G, Kohl DH (1986) Information derived from variation in the natural abundance of 15N in complex biological systems. In: Buncel E, Saunders WH (eds) Heavy atom isotope effects. Elsevier, Amsterdam, pp 133–190
Sheidegger Y, Saurer M, Bahn M, Siegwolf (2000) Linking stable oxygen and carbon isotopes with stomatal conductance and photosynthetic capacity: a conceptual model. Oecologia 125:350–357
Smith BN, Epstein S (1971) Two categories of 13C/12C ratios for higher plants. Plant Physiol 47:380–384
Smith EC, Griffiths H (1996) The occurrence of the chloroplast pyrenoide is correlated with the activity of a CO2-concentration mechanism and carbon isotope discrimination in lichens and bryophytes. Planta 198:6–16
Smith EC, Griffiths H (1998) Intraspecific variation in photosynthetic responses of treboixioid lichens with reference to the activity of a carbon-concentrating mechanism. Oecologia 113:360–369
Sparks JP, Ehleringer JR (1997) Leaf carbon isotope discrimination and nitrogen content for riparian trees along elevational transects. Oecologia 109:362–367
Sternberg L, Swart P (1987) Utilization of fresh water and ocean water by coastal plants of southern Florida. Ecology 68:1898–1905
Tans PP, Monk WG (1980) Past atmospheric CO2 levels and the 13C/12C ratios in tree rings. Tellus 32:268–283
Teeri JA (1981) Stable carbon isotopes analysis of mosses and lichens growing in xeric and moist habitats. Bryologist 84:82–82
Thorburn P, Walker G (1993) Groundwater or streams? In: Ehleringer J, Hall AE, Farquhar GD (eds) Stable isotopes and plant carbon-water relations. Academic Press, Oxford, pp 111–128
Valladares F, Ascaso C, Sancho LG (1998) Water storage in the lichen family Umbilicariaceae. Bot Acta 111:99–107
Vitousek PM, Field CB, Matson PA (1990) Variations in foliar δ13C in Hawaiian Metrosideros polymorpha: a case of internal resistance? Oecologia 84:362–370
Vogel JC (1980) Fractionation of the carbon isotopes during photosynthesis. Springer, Berlin Heidelberg New York, 29 pp
Von Caemmerer S, Evans J (1991) Determination of the average partial pressure of CO2 in chloroplasts from leaves of several C3 plants. Aust J Plant Physiol 18:287–305
Walcroft AS, Silvester WB, Grace J, Carson S, Warring R (1996). Effects of branch length on carbon isotope discrimination in Pinus radiata. Tree Physiol 16:281–286
Wallace CS, Rundel PW (1979) Sexual dimorphism and resource allocation in male and female shrubs of Simmondsia chinensis. Oecologia 44:34–39
Waring RH, Silvester W (1994) Variation in foliar δ13C values within tree crowns of Pinus radiata. Tree Physiol 14:1203–1213
Warren CR, McGrath JF, Adams MA (2001) Water availability and carbon isotope discrimination in conifers. Oecologia 127:476–486
Wershaw R, Friedman I, Heller S, Frank P (1966) Hydrogen isotope fractionation of water passing through trees. In: Hobson G, Spears G (eds) Advances in organic geochemistry. Pergamon Press, London, pp 55–67
White J, Cook E, Lawrence J, Broecker W (1985) The D/H ratios of sap in trees: implications of water sources and tree-rings D/H ratios. Geochim Cosmochim Act 49:237–246
Williams TG, Flanagan LB (1996) Effect of changes in water content on photosynthesis, respiration and discrimination against 13CO2 and C18O16O in Pleurozium and Sphagnum. Oecologia 108:38–46
Williams TG, Flanagan LB (1998) Measuring and modelling environmental influences on photosynthetic gas exchange in Shagnum and Pleurozium. Plant Cell Environ 21:555–564
Winter K, Holtum (2002) How closely do the 13C values of CAM plants reflect the proportion of CO2 fixed during day and night? Plant Physiol (in Press)
Yakir D (1998) Oxygen-18 in leaf water: a crossroad for plant-associated isotopic signals. In Griffiths H (ed) Stable isotopes, Integration of biological and geochemical processes. Bios Scientific, Oxford, pp 147–168
Yakir D, Sternberg LDL (2000) The use of stable isotopes to study ecosystem gas exchange. Oecologia 123:297
Yakir D, DeNiro MJ, Rundel PW (1989) Isotopes in homogeneity of leaf water: evidence and implications for the use of isotopic signals transduced by plants. Geochim Cosmochim 53:2769–2773
Zhang JW, Marshall JD (1995) Variation in carbon isotope discrimination and photosynthetic gas exchange among populations of Pseudotsuga menziesii and Pinus ponderosa in different environments. Funct Ecol 9:402–412
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Máguas, C., Griffiths, H. (2003). Applications of stable isotopes in plant ecology. In: Esser, K., Lüttge, U., Beyschlag, W., Hellwig, F. (eds) Progress in Botany. Progress in Botany, vol 64. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-55819-1_18
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