Abstract
Growth plate chondrocytes are the target of the hydroxylated vitamin D metabolites 1α,25(OH)2D3and 24,25(OH)2D3. Because studies on the production of these polar metabolites were inconclusive in various in vitro systems, the expression of a potential paracrine/autocrine vitamin D system was examined in primary cultures of rat growth plate chondrocytes using real-time RT-PCR. Compared to UMR cells and renal homogenates primary cultures of growth plate chondrocytes expressed low levels of 25-hydroxy-1a-hydroxylase as well as 25-hydroxy-24-hydroxylase. The expression of both is modulated by 25 vitamin D3, but 1α,25(OH)2D3affected only 25-hydroxy-24-hydroxylase. If these findings are confirmed in intact growth plates, the polar vitamin D metabolites could act in a paracrine/autocrine fashion within the growth plate.
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© 2003 Springer-Verlag Berlin Heidelberg
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Weber, L., Hügel, U., Reichrath, J., Sieverts, H., Mehls, O., Klaus, G. (2003). Cultured Rat Growth Plate Chondrocytes Express Low Levels of 1α-Hydroxylase. In: Reichrath, J., Tilgen, W., Friedrich, M. (eds) Vitamin D Analogs in Cancer Prevention and Therapy. Recent Results in Cancer Research, vol 164. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-55580-0_10
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DOI: https://doi.org/10.1007/978-3-642-55580-0_10
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-62435-3
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