Abstract
In the present chapter, we investigate a novel method for molecular manipulation and modification at the single-molecule level using microtechnologies, taking DNA as our target. A high-intensity high-frequency electrostatic field (≥ 106 V/m, ≈ 1 MHz) created in a microfabricated structure is used to immobilize DNA at a predetermined position on a solid surface with fully stretched conformation. A laser, a mechanical stylus, or an enzyme-immobilized probe is used to cut the stretched DNA at the target position. The cutting position of a restriction-enzyme probe is shown to agree with the restriction map, providing proof that the specificity of the enzyme is preserved even when both the enzyme and the target molecule are immobilized.
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© 2003 Springer-Verlag Berlin Heidelberg
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Washizu, M. (2003). Microsystems for Single-Molecule Handling and Modification. In: Fujita, H. (eds) Micromachines as Tools for Nanotechnology. Microtechnology and MEMS. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-55503-9_2
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DOI: https://doi.org/10.1007/978-3-642-55503-9_2
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-62465-0
Online ISBN: 978-3-642-55503-9
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