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Nucleic Acids in the Gas Phase pp 225–252Cite as

Ligand Binding to Nucleic Acids

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Part of the book series: Physical Chemistry in Action ((PCIA))

Abstract

Among the preeminent compounds that bind to DNA are numerous anticancer and antibacterial therapeutics. The development of new chemotherapeutics has accelerated the need for sensitive and versatile analytical techniques that are capable of characterizing DNA/ligand interactions including determination of binding stoichiometries, selectivities, and affinities. Electrospray ionization mass spectrometry (ESI-MS) has emerged as a useful technique for the analysis of complexes formed between DNA and small molecules due to its low sample consumption and fast analysis time. This chapter describes the exploration, optimization, and validation of ESI-MS methods for characterizing DNA–ligand interactions.

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Abbreviations

ESI:

Electrospray ionization

CID:

Collision-induced dissociation

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Acknowledgements

This work was supported by the Robert A. Welch Foundation (F-1155) and the National Institutes of Health (RO1 GM65956).

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Authors and Affiliations

  1. Department of Chemistry, University of Texas, Austin, TX, 78712, USA

    Jennifer S. Brodbelt & Zhe Xu

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  1. Jennifer S. Brodbelt
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  2. Zhe Xu
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Correspondence to Jennifer S. Brodbelt .

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  1. Université de Bordeaux, Institut Européen de Chimie et Biologie, Pessac, France

    Valérie Gabelica

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Brodbelt, J.S., Xu, Z. (2014). Ligand Binding to Nucleic Acids. In: Gabelica, V. (eds) Nucleic Acids in the Gas Phase. Physical Chemistry in Action. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-54842-0_9

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