Fragmentation Reactions of Nucleic Acid Ions in the Gas Phase

  • Yang Gao
  • Scott A. McLuckeyEmail author
Part of the Physical Chemistry in Action book series (PCIA)


This chapter summarizes literature describing the gas-phase fragmentation of nucleic acid ions under a variety of reaction conditions. Specifically, the phenomenology of gas-phase dissociation of nucleic acid ions is determined by ion type, charge state, the energy deposition method, and the fragmentation reaction timescale. Various proposed mechanisms are summarized. The chapter is organized by dissociation method. For the most extensively studied collision-induced dissociation (CID), the literature is subcategorized by analyte and ion type. In many cases, no single fragmentation mechanism can account for all the reported products. This suggests that multiple dissociation mechanisms can contribute, depending on ion type, ion charge state, and reaction conditions.


Nucleic acids Gas-phase fragmentation Electrospray ionization Matrix-assisted laser desorption/ionization collision-induced dissociation Infrared multiphoton dissociation Ultraviolet photodissociation Electron photodetachment dissociation 



Blackbody infrared radiative dissociation


Collision-induced dissociation




Double resonance


Electron capture dissociation


Electron detachment dissociation


Electron photodetachment dissociation


Electrospray ionization


Electron transfer dissociation


Electron transfer collision-activated dissociation


Electron transfer-infrared multiphoton dissociation


Electron transfer-ultraviolet photodissociation


Fast atom bombardment


Fourier transform ion cyclotron resonance


Ionization potential




Infrared multiphoton dissociation


Locked nucleic acid


Matrix-assisted laser desorption/ionization


Mixed-backbone oligonucleotide




Mass spectrometer/mass spectrometry


Negative electron transfer dissociation


Negative ion electron capture dissociation




Proton affinity


Plasma desorption




Post-source decay


Quadrupole/time-of-flight tandem mass spectrometer






RNA interference


Surface-induced dissociation


Single nucleotide polymorphisms


Sustained off-resonance irradiation




Tandem time-of-flight




Ultraviolet photodissociation


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Department of ChemistryPurdue UniversityWest LafayetteUSA

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