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Gas-Phase Spectroscopy of Nucleic Acids

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Book cover Nucleic Acids in the Gas Phase

Part of the book series: Physical Chemistry in Action ((PCIA))

Abstract

We describe here frequency-resolved gas-phase spectroscopy of nucleic acids. Frequency resolved means that the effect of photons on the nucleic acid molecules is measured as a function of the photon frequency. The present chapter is primarily focused on experimental aspects, and intended as a compass to navigate a rather interdisciplinary field. Indeed, gas-phase spectroscopy usually combines photonics, mass spectrometry (when ions are detected), and theoretical chemistry. Although theory is of prime importance for the interpretation of the results, as it is the comparison between experimental and theoretical energies of the resonance transitions that allow the structural interpretation of the experimental spectra, extended discussion of theory levels will not be provided here, but relevant literature will be indicated along the text. We will cover rotational, vibrational, and electronic spectroscopy from isolated nucleobases to oligonucleotides and nucleic acid higher-order structures.

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Abbreviations

CD:

Circular dichroism

CID:

Collision-induced dissociation

CLIO:

Centre Laser Infrarouge d’Orsay

FELIX:

Free-Electron Lasers for Infrared eXperiments

FTICRMS:

Fourier transform ion cyclotron resonance mass spectrometry

IC:

Internal conversion

IP:

Ionization potential

IR:

Infrared

IRMPD:

Infrared multiple photon dissociation

IR-UV:

Infrared-ultraviolet double resonance spectroscopy

IVR:

Intramolecular vibrational energy redistribution

LA-MB-FTMW:

Laser ablation molecular beam Fourier transform microwave spectroscopy

LIF:

Laser-induced fluorescence

NMR:

Nuclear magnetic resonance

PD:

Photodissociation

R1PI:

Resonance-enhanced single-photon ionization

R2PI:

Resonance-enhanced two-photon ionization

REMPI:

Resonance-enhanced multiphoton ionization

S 0 :

Electronic ground state

S 1 :

First electronically excited state

UV:

Ultraviolet

UVMPD:

Ultraviolet multiple photon dissociation

UV-UV:

Ultraviolet–ultraviolet double resonance spectroscopy

Vis:

Visible

VUV:

Vacuum ultraviolet

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Author information

Authors and Affiliations

  1. IECB, ARNA Laboratory, Univ. Bordeaux, 33600, Pessac, France

    Valérie Gabelica

  2. U869, ARNA Laboratory, Inserm, 33000, Bordeaux, France

    Valérie Gabelica

  3. UMS 3033 and Inserm US001, IECB, CNRS, 33600, Pessac, France

    Frédéric Rosu

Authors
  1. Valérie Gabelica
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  2. Frédéric Rosu
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Correspondence to Valérie Gabelica .

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Editors and Affiliations

  1. Université de Bordeaux, Institut Européen de Chimie et Biologie, Pessac, France

    Valérie Gabelica

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Gabelica, V., Rosu, F. (2014). Gas-Phase Spectroscopy of Nucleic Acids. In: Gabelica, V. (eds) Nucleic Acids in the Gas Phase. Physical Chemistry in Action. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-54842-0_5

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