Skip to main content
SpringerLink
Log in

Fine Needle Aspiration Biopsy

  • Chapter
  • First Online:
Clinical Ophthalmic Oncology

Abstract

Fine needle aspiration biopsy (FNAB) of tumors has a long history. The first intraocular biopsy was performed by Hirschberg in 1868. Since the publication of a major report by Jakobiec in 1979, FNAB is increasingly used in the evaluation of ophthalmic tumors. The safety and reliability of ophthalmic FNAB have been reported by several other investigators with adequacy rates of 88–95 %.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Long SR, Cohen MB. Classics in cytology VII: Kun, Lebert, and early efforts at fine-needle aspiration biopsy. Diagn Cytopathol. 1996;14(2):182–3.

    Article  CAS  PubMed  Google Scholar 

  2. Webb AJ. Early microscopy: history of fine needle aspiration (FNA) with particular reference to goitres. Cytopathology. 2001;12(1):1–6.

    Article  CAS  PubMed  Google Scholar 

  3. Ansari NA, Derias NW. Fine needle aspiration cytology. J Clin Pathol. 1997;50(7):541–3.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  4. Rosa M. Fine-needle aspiration biopsy: a historical overview. Diagn Cytopathol. 2008;36(11):773–5.

    Article  PubMed  Google Scholar 

  5. Frable WJ. Fine-needle aspiration biopsy: a review. Hum Pathol. 1983;14(1):9–28.

    Article  CAS  PubMed  Google Scholar 

  6. Hajdu SI, Ehya H. Foundation of diagnostic cytology. Ann Clin Lab Sci. 2008;38(3):296–9.

    PubMed  Google Scholar 

  7. Veasey Jr CA. Intraocular biopsy. Am J Ophthalmol. 1951;34(3):432–4.

    PubMed  Google Scholar 

  8. Long JC, Black WC, Danielson RW. Aspiration biopsy in intraocular tumors. AMA Arch Ophthalmol. 1953;50(3):303–10.

    Article  CAS  PubMed  Google Scholar 

  9. Jensen OA, Andersen SR. Late complications of biopsy in intraocular tumors. Acta Ophthalmol. 1959;37:568–75.

    Article  CAS  Google Scholar 

  10. Takemura T, Tomatsu Y. Studies on malignant melanoma of the choroid. I. Needle biopsy, light microscopy, and electron microscopy. Nippon Ganka Gakkai zasshi. 1967;71(8):1317–22.

    CAS  PubMed  Google Scholar 

  11. Makley TA, Jr. Biopsy of intraocular lesions. Am J ophthalmology. 1967;64(3):Suppl:591–9.

    Google Scholar 

  12. Sagiroglu Jr N, Ozgonul T, Muderris S. Diagnostic intraocular cytology. Acta Cytol. 1975;19(1):32–7.

    CAS  PubMed  Google Scholar 

  13. Grgic Z, Ljustina-Ivancic N. [Cytomorphologic findings in the diagnosis of intraocular lymphocytic tumors (author’s transl)]. Klin Monbl Augenheilkd. 1978;173(3):416–8.

    CAS  PubMed  Google Scholar 

  14. Jakobiec FA, Coleman DJ, Chattock A, Smith M. Ultrasonically guided needle biopsy and cytologic diagnosis of solid intraocular tumors. Ophthalmology. 1979;86(9):1662–81.

    Article  CAS  PubMed  Google Scholar 

  15. Czerniak B, Woyke S, Domagala W, Krzysztolik Z. Fine needle aspiration cytology of intraocular malignant melanoma. Acta Cytol. 1983;27(2):157–65.

    CAS  PubMed  Google Scholar 

  16. Char DH, Miller TR. Fine needle biopsy in retinoblastoma. Am J Ophthalmol. 1984;97(6):686–90.

    CAS  PubMed  Google Scholar 

  17. Augsburger JJ, Shields JA, Folberg R, et al. Fine needle aspiration biopsy in the diagnosis of intraocular cancer. Cytologic-histologic correlations. Ophthalmology. 1985;92(1):39–49.

    Article  CAS  PubMed  Google Scholar 

  18. Midena E, Segato T, Piermarocchi S, Boccato P. Fine needle aspiration biopsy in ophthalmology. Surv Ophthalmol. 1985;29(6):410–22.

    Article  CAS  PubMed  Google Scholar 

  19. Shields JA, Shields CL, Ehya H, et al. Fine-needle aspiration biopsy of suspected intraocular tumors. The 1992 Urwick Lecture. Ophthalmology. 1993;100(11):1677–84.

    Article  CAS  PubMed  Google Scholar 

  20. Eide N, Syrdalen P, Walaas L, Hagmar B. Fine needle aspiration biopsy in selecting treatment for inconclusive intraocular disease. Acta Ophthalmol Scand. 1999;77(4):448–52.

    Article  CAS  PubMed  Google Scholar 

  21. Eide N, Walaas L. Fine-needle aspiration biopsy and other biopsies in suspected intraocular malignant disease: a review. Acta Ophthalmol. 2009;87(6):588–601.

    Article  PubMed  Google Scholar 

  22. Char DH, Miller T. Accuracy of presumed uveal melanoma diagnosis before alternative therapy. Br J Ophthalmol. 1995;79(7):692–6.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  23. Singh AD, Pelayes DE, Brainard JA, Biscotti CV. History, indications, techniques and limitations. Monogr Clin Cytol. 2012;21:1–9.

    Article  PubMed  Google Scholar 

  24. Biscotti C, Singh AD. FNA cytology of ophthalmic tumors, vol. 21. Basel: Karger; 2012.

    Google Scholar 

  25. Char DH, Miller TR, Ljung BM, et al. Fine needle aspiration biopsy in uveal melanoma. Acta Cytol. 1989;33(5):599–605.

    CAS  PubMed  Google Scholar 

  26. Davila RM, Miranda MC, Smith ME. Role of cytopathology in the diagnosis of ocular malignancies. Acta Cytol. 1998;42(2):362–6.

    Article  CAS  PubMed  Google Scholar 

  27. Faulkner-Jones BE, Foster WJ, Harbour JW, et al. Fine needle aspiration biopsy with adjunct immunohistochemistry in intraocular tumor management. Acta Cytol. 2005;49(3):297–308.

    Article  PubMed  Google Scholar 

  28. Shields CL, Manquez ME, Ehya H, et al. Fine-needle aspiration biopsy of iris tumors in 100 consecutive cases: technique and complications. Ophthalmology. 2006;113(11):2080–6.

    Article  PubMed  Google Scholar 

  29. Char DH, Kemlitz AE, Miller T, Crawford JB. Iris ring melanoma: fine needle biopsy. Br J Ophthalmol. 2006;90(4):420–2.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  30. Fastenberg DM, Finger PT, Chess Q, et al. Vitrectomy retinotomy aspiration biopsy of choroidal tumors. Am J Ophthalmol. 1990;110(4):361–5.

    CAS  PubMed  Google Scholar 

  31. Arbour JD, Mukai S. Biopsy of the retina and the choroid. Int Ophthalmol Clin. 1999;39(1):213–22.

    Article  CAS  PubMed  Google Scholar 

  32. Foulds WS. The uses and limitations of intraocular biopsy. Eye. 1992;6(Pt 1):11–27.

    Article  PubMed  Google Scholar 

  33. Bechrakis NE, Foerster MH, Bornfeld N. Biopsy in indeterminate intraocular tumors. Ophthalmology. 2002;109(2):235–42.

    Article  PubMed  Google Scholar 

  34. Kvanta A, Seregard S, Kopp ED, et al. Choroidal biopsies for intraocular tumors of indeterminate origin. Am J Ophthalmol. 2005;140(6):1002–6.

    Article  PubMed  Google Scholar 

  35. Sen J, Groenewald C, Hiscott PS, et al. Transretinal choroidal tumor biopsy with a 25-gauge vitrector. Ophthalmology. 2006;113(6):1028–31.

    Article  PubMed  Google Scholar 

  36. Char DH. Intraocular biopsy. In: Singh AD, Damato BE, Pe’er J, et al., editors. Clinical ophthalmic oncology. Philadelphia: Saunders-Elsevier; 2007.

    Google Scholar 

  37. Green WR. Diagnostic cytopathology of ocular fluid specimens. Ophthalmology. 1984;91(6):726–49.

    Article  CAS  PubMed  Google Scholar 

  38. Augsburger JJ, Shields JA. Fine needle aspiration biopsy of solid intraocular tumors: indications, instrumentation and techniques. Ophthalmic Surg. 1984;15(1):34–40.

    CAS  PubMed  Google Scholar 

  39. Pelayes DE, Zarate JO, Biscotti CV, Singh AD. Calibrated needle for ophthalmic fine needle aspiration biopsy. Br J Ophthalmol. 2012;96(8):1147–8.

    Article  PubMed  Google Scholar 

  40. Shields JA, Shields CL, Ehya H, et al. Fine-needle aspiration biopsy of suspected intraocular tumors. Int Ophthalmol Clin. 1993;33(3):77–82.

    Article  CAS  PubMed  Google Scholar 

  41. Young TA, Burgess BL, Rao NP, et al. Transscleral fine-needle aspiration biopsy of macular choroidal melanoma. Am J Ophthalmol. 2008;145(2):297–302.

    Article  PubMed  Google Scholar 

  42. Singh AD, Pelayes D, Zarate JO, Biscotti CV. FNAB of uveal melanoma with a graded prototype needle. ARVO Meet Abstr. 2011;52(6):3270.

    Google Scholar 

  43. Akgul H, Otterbach F, Bornfeld N, Jurklies B. Intraocular biopsy using special forceps: a new instrument and refined surgical technique. Br J Ophthalmol. 2011;95(1):79–82.

    Article  PubMed  Google Scholar 

  44. Grossniklaus HE. Fine-needle aspiration biopsy of the iris. Arch Ophthalmol. 1992;110(7):969–76.

    Article  CAS  PubMed  Google Scholar 

  45. Matthews BJ, Mudhar HS, Rennie IG. Trans-corneal fine cannula aspiration: Rycroft cannula aspiration technique for sampling iris tumours. Br J Ophthalmol. 2012;96(3):329–31.

    Article  PubMed  Google Scholar 

  46. Brainard JA, Biscotti CV. Cytological preparation. Monogr Clin Cytol. 2012;21:10–6.

    Article  PubMed  Google Scholar 

  47. Pelayes DE, Zarate JO. Fine needle aspiration biopsy with liquid-based cytology and adjunct immunohistochemistry in intraocular melanocytic tumors. Eur J Ophthalmol. 2010;20(6):1059–65.

    PubMed  Google Scholar 

  48. Seregard S. To biopsy or not to biopsy? Acta Ophthalmol. 2009;87(6):586–7.

    Article  PubMed  Google Scholar 

  49. Augsburger JJ, Correa ZM. Taxonomy of intraocular tumor biopsies. Implications for reporting.and comparison of results. IOVS 2012;53:ARVO E-Abstract 3397 2012.

    Google Scholar 

  50. Biscotti CV, Singh AD. Uveal melanoma: diagnostic features. Monogr Clin Cytol. 2012;21:44–54.

    Article  PubMed  Google Scholar 

  51. Biscotti CV, Singh AD. Uveal metastases. Monogr Clin Cytol. 2012;21:17–30.

    Article  PubMed  Google Scholar 

  52. Turell ME, Tubbs RR, Biscotti CV, Singh AD. Uveal melanoma: prognostication. Monogr Clin Cytol. 2012;21:55–60.

    Article  PubMed  Google Scholar 

  53. Augsburger JJ, Correa ZM, Schneider S, et al. Diagnostic transvitreal fine-needle aspiration biopsy of small melanocytic choroidal tumors in nevus versus melanoma category. Trans Am Ophthalmol Soc. 2002;100:225–32; discussion 32–4.

    PubMed Central  PubMed  Google Scholar 

  54. Ylagan LR. Intraocular pigmented proliferations in the context of cytologic evaluation. Diagn Cytopathol. 2009;37(11):853–64.

    Article  PubMed  Google Scholar 

  55. Cohen VM, Dinakaran S, Parsons MA, Rennie IG. Transvitreal fine needle aspiration biopsy: the influence of intraocular lesion size on diagnostic biopsy result. Eye (Lond). 2001;15(Pt 2):143–7.

    Article  CAS  Google Scholar 

  56. Shields CL, Ganguly A, Materin MA, et al. Chromosome 3 analysis of uveal melanoma using fine-needle aspiration biopsy at the time of plaque radiotherapy in 140 consecutive cases: the Deborah Iverson, MD, Lectureship. Arch Ophthalmol. 2007;125(8):1017–24.

    Article  PubMed  Google Scholar 

  57. Augsburger JJ. Fine needle aspiration biopsy of suspected metastatic cancers to the posterior uvea. Trans Am Ophthalmol Soc. 1988;86:499–560.

    CAS  PubMed Central  PubMed  Google Scholar 

  58. Shields CL, Shields JA, Gross NE, et al. Survey of 520 eyes with uveal metastases. Ophthalmology. 1997;104(8):1265–76.

    Article  CAS  PubMed  Google Scholar 

  59. Trichopoulos N, Augsburger JJ. Neuroendocrine tumours metastatic to the uvea: diagnosis by fine needle aspiration biopsy. Graefes Arch Clin Exp Ophthalmol. 2006;244(4):524–8.

    Article  PubMed  Google Scholar 

  60. Char DH, Ljung BM, Deschenes J, Miller TR. Intraocular lymphoma: immunological and cytological analysis. Br J Ophthalmol. 1988;72(12):905–11.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  61. Davis JL, Solomon D, Nussenblatt RB, et al. Immunocytochemical staining of vitreous cells. Indications, techniques, and results. Ophthalmology. 1992;99(2):250–6.

    Article  CAS  PubMed  Google Scholar 

  62. Davis JL, Viciana AL, Ruiz P. Diagnosis of intraocular lymphoma by flow cytometry. Am J Ophthalmol. 1997;124(3):362–72.

    CAS  PubMed  Google Scholar 

  63. Farkas T, Harbour JW, Davila RM. Cytologic diagnosis of intraocular lymphoma in vitreous aspirates. Acta Cytol. 2004;48(4):487–91.

    Article  PubMed  Google Scholar 

  64. Karcioglu ZA. Fine needle aspiration biopsy (FNAB) for retinoblastoma. Retina. 2002;22(6):707–10.

    Article  PubMed  Google Scholar 

  65. Ali MJ, Honavar SG, Vemuganti GK, Singh AD. Fine needle aspiration biopsy of retinal tumors. Monogr Clin Cytol. 2012;21:72–81.

    Article  PubMed  Google Scholar 

  66. Seregard S, Tani E. Fine needle aspiration cytology in orbital tumors. Monogr Clin Cytol. 2012;21:82–9.

    Article  PubMed  Google Scholar 

  67. Turell ME, Hayden BC, McMahon JT, et al. Uveal schwannoma surgery. Ophthalmology. 2009;116(1):163–e6.

    Article  PubMed  Google Scholar 

  68. Eriksson O, Hagmar B, Ryd W. Effects of fine-needle aspiration and other biopsy procedures on tumor dissemination in mice. Cancer. 1984;54(1):73–8.

    Article  CAS  PubMed  Google Scholar 

  69. Liebens F, Carly B, Cusumano P, et al. Breast cancer seeding associated with core needle biopsies: a systematic review (Structured abstract). Maturitas. 2009;62(2):113–23.

    Article  PubMed  Google Scholar 

  70. Polyzos SA, Anastasilakis AD. A systematic review of cases reporting needle tract seeding following thyroid fine needle biopsy. World J Surg. 2010;34(4):844–51.

    Article  PubMed  Google Scholar 

  71. Glasgow BJ, Brown HH, Zargoza AM, Foos RY. Quantitation of tumor seeding from fine needle aspiration of ocular melanomas. Am J Ophthalmol. 1988;105(5):538–46.

    CAS  PubMed  Google Scholar 

  72. Karcioglu ZA, Gordon RA, Karcioglu GL. Tumor seeding in ocular fine needle aspiration biopsy. Ophthalmology. 1985;92(12):1763–7.

    Article  CAS  PubMed  Google Scholar 

  73. Char DH, Kemlitz AE, Miller T. Intraocular biopsy. Ophthalmol Clin North Am. 2005;18(1):177–85, x.

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Cole Eye Institute, Cleveland Clinic Foundation, Cleveland, OH, USA

    Arun D. Singh MD

  2. Department of Anatomic Pathology, Cleveland Clinic Foundation, Cleveland, OH, USA

    Charles V. Biscotti MD

  3. Department of Ophthalmology, University of Buneos Aires, Buenos Aires, Argentina

    David E. Pelayes MD

Authors
  1. Arun D. Singh MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Charles V. Biscotti MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. David E. Pelayes MD
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to David E. Pelayes MD .

Editor information

Editors and Affiliations

  1. Ocular Oncology Service, Departments of Ophthalmology and Radiation Oncology, University of California, San Francisco, San Francisco, California, USA

    Bertil Damato

  2. Dept. of Ophthalmic Oncology, Cole Eye Institute Cleveland Clinic Foundation, Cleveland, Ohio, USA

    Arun D. Singh

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Singh, A.D., Biscotti, C.V., Pelayes, D.E. (2014). Fine Needle Aspiration Biopsy. In: Damato, B., Singh, A. (eds) Clinical Ophthalmic Oncology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-54255-8_3

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-54255-8_3

  • Published:

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-54254-1

  • Online ISBN: 978-3-642-54255-8

  • eBook Packages: MedicineMedicine (R0)

Publish with us

Policies and ethics

Search

Navigation