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Haploid and Doubled Haploid Plant Production in Gentian (Gentiana spp.)

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The Gentianaceae - Volume 2: Biotechnology and Applications

Abstract

Successful production of haploid and doubled haploid (DH) plants from male and female gametophytic cells by in vitro culture has been established in some gentians ( Gentiana triflora , G. scabra , and their hybrid), which are used as ornamental plants . Plant regeneration is obtained by both anther culture (androgenesis) and unfertilized ovule/ovary culture (gynogenesis). Attention is given to culture techniques, factors influencing androgenesis and gynogenesis, the ploidy of regenerants, and utilization of the molecular genetic marker for identification of DH. Although plant regeneration is obtained by both anther culture and unfertilized ovule/ovary culture , the latter has more advantages than the former. In gentian, the establishment of the haploid and DH method has application to F1 breeding and also to basic genetic studies.

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Authors and Affiliations

  1. Faculty of Agriculture, Iwate University, Morioka, 020-8550, Japan

    Hisako Doi & Yoshihito Takahata

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  1. Hisako Doi
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  2. Yoshihito Takahata
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Correspondence to Yoshihito Takahata .

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Editors and Affiliations

  1. Botanical Garden-Center for Biological Diversity Conservation, Polish Academy of Sciences, Warsaw, Poland

    Jan J. Rybczyński

  2. Plant and Crop Sciences Division, University of Nottingham School of Biosciences, Loughborough, United Kingdom

    Michael R. Davey

  3. Botanical Garden-Center for Biological Diversity Conservation, Polish Academy of Sciences, Warsaw, Poland

    Anna Mikuła

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Doi, H., Takahata, Y. (2015). Haploid and Doubled Haploid Plant Production in Gentian (Gentiana spp.). In: Rybczyński, J., Davey, M., Mikuła, A. (eds) The Gentianaceae - Volume 2: Biotechnology and Applications. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-54102-5_8

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