Abstract
Comparative investigations have been carried out into total flavonoids and xanthones in cultured tissues, isolated cultured roots , and wild plants of Gentiana species from the Ukrainian flora. The capacity was ascertained for synthesizing these biologically active substances in vitro. The compounds varied both in calli and isolated roots derived from plants of different gentian species, as well as in tissue and organ cultures of the same species generated from different plants. Morphogenic and non-morphogenic cultures showed much lower flavonoid and xanthone content than shoots of intact plants, but more or close to that of natural roots. Isolated root cultures in most cases displayed greater concentrations of these biologically active compounds than callus. A high yield of biomass from gentian cultures in vitro and their ability to synthesize and accumulate flavonoids and xanthones enable them to be considered as a promising source of these biologically active compounds.
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The authors would like to thank Iryna Petrusha (Foreign Languages Department, Institute of International Relations, Kyiv National Taras Shevchenko University) for assistance in translating the text into English.
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Drobyk, N.M., Mel’nyk, V.M., Twardovska, M.O., Konvalyuk, I.I., Kunakh, V.A. (2015). Tissue and Organ Cultures of Gentians as Potential Sources of Xanthones and Flavonoids. In: Rybczyński, J., Davey, M., Mikuła, A. (eds) The Gentianaceae - Volume 2: Biotechnology and Applications. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-54102-5_13
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DOI: https://doi.org/10.1007/978-3-642-54102-5_13
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