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Procedure for Evaluation of a Liquid Phase Radioimmunoassay Determining Immune Complexes in Sera

  • Conference paper
Immunology and Epidemiology

Part of the book series: Lecture Notes in Biomathematics ((LNBM,volume 65))

  • 167 Accesses

Abstract

Employment of the optimal mathematical procedure for determining a standard curve is essential for a proper evaluation of laboratory tests. These test results are then frequently calculated with the use of a standard curve, which expresses the relationship between the dose and response. This approach is also used in a liquid phase radioimmunoassay which employes binding of iodinated (125I) Clq as a measure of the presence of immunological complexes in serum samples. The present work describes our approach for calculation of the predicted variance — which is a rational for giving weights to empirical points composing the standard curve.

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References

  1. Chen, L-W., Heminger, L., Maxon, H.R., Tsay, J.Y. (1980) Non-specific binding as a source of error in thyrotropin radioimmunoassay with polyethylene glycol as separating agent. Clin. Chem. 26:487–490.

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  4. Łaba, C., Haas, H., Jodkowski, J.T., Lange, A. (1986) Statistical analysis of a liquid phase radioimmunoassay for immune complex determination. Arch. Arch. Immunol. Ther. Exp., in press

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  5. Rodbard, D., Hutt, D.M. (1974) Statistical analysis of radioimmunoassays and immunoradiometric (labelled antibody) assays: A generalized weighted, iterative, least squares method for logistic curve fitting. In: Radioimmunoassay and Related procedures in Medicine. Proceedings of a Symposium, Istanbul 1973, Vol.I., IAEA, Vienna 165–192

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Author information

Authors and Affiliations

  1. Dept. of Clinical Immunology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland

    C. Łaba & A. Lange

  2. Borstel Research Institute, 2061, Borstel, Germany

    H. Haas

  3. Dept. of Physical Chemistry, Medical School of Wrocław, Wrocław, Poland

    J. T. Jodkowski

Authors
  1. C. Łaba
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  2. H. Haas
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  3. J. T. Jodkowski
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  4. A. Lange
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Editor information

Editors and Affiliations

  1. Departments of Physics and Microbiology, The University of British Columbia, Vancouver, B.C., V6T 1W5, Canada

    Geoffrey W. Hoffmann

  2. Institute of Molecular Genetics, Czechoslovak Academy of Sciences, 142 20, Prague, Czechoslavakia

    Tomáš Hraba

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© 1986 Springer-Verlag Berlin Heidelberg

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Łaba, C., Haas, H., Jodkowski, J.T., Lange, A. (1986). Procedure for Evaluation of a Liquid Phase Radioimmunoassay Determining Immune Complexes in Sera. In: Hoffmann, G.W., Hraba, T. (eds) Immunology and Epidemiology. Lecture Notes in Biomathematics, vol 65. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-51691-7_14

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  • DOI: https://doi.org/10.1007/978-3-642-51691-7_14

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-16431-9

  • Online ISBN: 978-3-642-51691-7

  • eBook Packages: Springer Book Archive

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