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Enzymes as Indicators of Intracellular Reaction Conditions

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Quantitative Biology of Metabolism

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Abstract

In contrast to numerous data concerning enzyme activity in vitro, little is known in a quantitative sense about enzymes inside the cell. As an example of enzyme kinetics in vivo the urea-urease-system of plant cells was investigated. The enzyme located in the groundplasm shows kinetics similar to that in water solution: the Michaelis constant and the activation energy in vivo are of the same magnitude as in vitro. — Furthermore, a specific inhibition of urease by chloramphenicol takes place in vitro. This effect enables the free concentration of the antibiotic in the groundplasm to be roughly estimated.

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Authors
  1. E. Müller
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Editors and Affiliations

  1. Institut für Strahlenschutz, Reaktorzentrum Seibersdorf (Österr. Studiengesellschaft für Atomenergie GmbH), Lenaugasse 10, A 1082, Wien, Österreich

    A. Locker

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© 1968 Springer-Verlag Berlin · Heidelberg

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Müller, E. (1968). Enzymes as Indicators of Intracellular Reaction Conditions. In: Locker, A. (eds) Quantitative Biology of Metabolism. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-51065-6_21

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  • DOI: https://doi.org/10.1007/978-3-642-51065-6_21

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-04301-0

  • Online ISBN: 978-3-642-51065-6

  • eBook Packages: Springer Book Archive

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