Skip to main content
SpringerLink
Log in

Serological Recognition of Toxoplasma gondii Cyst Antigens

  • Chapter
Book cover Toxoplasma gondii

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 219))

Abstract

Infection with Toxoplasma gondii is characterised by an acute phase, associated with the rapid proliferation of tachyzoites, followed by a chronic phase during which the slow-growing bradyzoite stage forms cysts in brain and muscle tissue. Although tissue cysts are often refered to as ‘dormant’, there is evidence of bradyzoite turnover within the cyst (Pavesio et al. 1992) and of periodic cyst rupture (Ferguson et al. 1989). Bradyzoites released from the cyst may convert into tachyzoites causing recrudesence of acute disease which, in immunosuppressed individuals, can be fatal (Luft and Remington 1992). Control of disease relies upon recognition and immunoregulation of both stages and it is therefore important to have an understanding of the comparative antigenic structure of tachyzoites and bradyzoites.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bohne W, Heesemann J, Gross U (1993) Coexistence of heterogeneous populations of Toxoplasma gondii parasites within parasitophorous vacuoles of murine macrophages revealed by a bradyzoite-specific monoclonal antibody. Parasitol Res 79:485–487

    Article  PubMed  CAS  Google Scholar 

  • Cesbron-Delauw MF (1994) Dense granule organelles of Toxoplasma gondii. Parasitol Today 10:293–296

    Article  PubMed  CAS  Google Scholar 

  • Couvreur G, Sadak A, Fortier B, Dubremetz JF (1988) Surface antigens of Toxoplasma gondii. Parasitol 97:1–10

    Article  Google Scholar 

  • Darcy F, Charif H, Caron H, Deslee D, Pierce RJ, Cesbron-Delauw MF, Decoster A, Capron A (1990) Identification and biochemical characterisation of antigens of tachyzoites and bradyzoites of Toxoplasma gondii with cross-reactive epitopes. Parasitol Res 76:473–478

    Article  PubMed  CAS  Google Scholar 

  • Ferguson DJP, Hutchinson WM (1987) An ultrastructural study of the early development and tissue cyst formation of Toxoplasma gondii in the brains of mice. Parasitol Res 73:483–491

    Article  PubMed  CAS  Google Scholar 

  • Ferguson DJP, Hutchinson WM, Pettersen E (1989) Tissue cyst rupture in mice chronically infected with Toxoplasma gondii. Parasitol Res 75:599–603

    Article  PubMed  CAS  Google Scholar 

  • Ferguson DJP, Graham DI, Hutchinson WM (1991) Pathological changes in the brains of mice infected with Toxoplasma gondii: a histological, immunocytochemical and ultrastructural study. Int J Exp Path 72:463–474

    CAS  Google Scholar 

  • van Gelder P, Bosman F, Demeuter F, van Heuverswyn H, Henion P (1993) Serodiagnosis of Toxoplasma gondii by using a recombinant form of a 54 kDa rhoptry antigen expressed in E. coll. J Clin Microbiol 31:9–15

    PubMed  Google Scholar 

  • Grimwood J, Smith JE (1995) Toxoplasma gondii: redistribution of tachyzoite surface protein during host cell invasion and intracellular development. Parasitol Res 81:657–661

    Article  PubMed  CAS  Google Scholar 

  • Kasper LH (1989) Identification of stage specific antigens of Toxoplasma gondii. Infect Immun 57:668–672

    PubMed  CAS  Google Scholar 

  • Kasper LH, Currie KM, Bradley MS (1985) An unexpected response to vaccination with a purified major membrane tachyzoite antigen (P30) of Toxoplasma gondii. J Immunol 132:443–449

    Google Scholar 

  • Luft BJ, Remington JS (1992) Toxoplasmic encephalitis in AIDS. Clin Infect Dis 15:211–222

    Article  PubMed  CAS  Google Scholar 

  • Lunde MN, Jacobs L (1983) Antigenic differences between endozoites and cystozoites of Toxoplasma gondii. J Parasitol 69:806–811

    Article  PubMed  CAS  Google Scholar 

  • Makioka AA, Suzuki Y, Kobayashi A (1991) Recognition of tachyzoite and bradyzoite antigens of Toxoplasma gondii by infected hosts. Infect Immun 59:2763–2766

    PubMed  CAS  Google Scholar 

  • Parmley SF, Yang S, Harth G, Sibley LD, Sucharczuk A, Remington JS (1994) Molecular characterisation of a 65-kilodalton Toxoplasma gondii antigen expressed abundantly in the matrix of tissue cysts. Mol Biochem Parasitol 66:283–296

    Article  PubMed  CAS  Google Scholar 

  • Partenen P, Turunen HJ, Paasivuo RAT, Leinikki PO (1984) Immunoblot analysis of Toxoplasma gondii antigens by human immunoglobulins G, M and A antibodies at different stages of infection. J Clin Microbiol 20: 133–135

    Google Scholar 

  • Pavesio CEN, Chiappino ML, Setzer PY, Nichols BA (1992) Toxoplasma gondii: differentiation and death of bradyzoites. Parasitol Res 78: 1–9

    Article  PubMed  CAS  Google Scholar 

  • Potasmen I, Araujo FG, Desmonts G, Remington JS (1986) Analysis of Toxoplasma gondii antigens recognised by human sera obtained before and after acute infection. J Infect Dis 154:650–657

    Article  Google Scholar 

  • Tomavo S, Fortier B, Soête M, Ansel C, Camus D, Dubremetz JF (1991) Characterisation of bradyzoite-specific antigens of Toxoplasma gondii. Infect Immun 59:3750–3753

    PubMed  CAS  Google Scholar 

  • Torpier G, Charif H, Darcy F, Liu J, Dardé ML, Capron A (1993) Toxoplasma gondii: differential location of antigens secreted from encysted bradyzoites. Exp Parasitol 77: 13–22

    Article  PubMed  CAS  Google Scholar 

  • Verhofstede C, van Gelder P, Rabaey M (1988) The infection stage-related IgG response to Toxoplasma gondii studied by immunoblotting. Parasitol Res 74:516–520

    Article  PubMed  CAS  Google Scholar 

  • Weiss LM, LaPlace D, Tanowitz HB, Wittner M (1992) Identification of Toxoplasma gondii bradyzoite specific monoclonal antibodies. J Infect Dis 166:213–215

    Article  PubMed  CAS  Google Scholar 

  • Woodison G, Smith JE (1990) Identification of the dominant cyst antigens of Toxoplasma gondii. Parasitol 100:389–342

    Article  Google Scholar 

  • Woodison G, Balfour AH, Smith JE (1993) Sequential reactivity of serum against cyst antigens in Toxoplasma infection. J Clin Pathol 46:548–550

    Article  PubMed  CAS  Google Scholar 

  • Zhang YW, Smith JE (1995a) Toxoplasma gondii: Reactivity of murine sera against tachyzoite and cyst antigens via FAST-ELISA. Int J Parasitol 25:637–640

    Article  PubMed  CAS  Google Scholar 

  • Zhang YW, Smith JE (1995b) Toxoplasma gondii: Identification and characterisation of a cyst molecule. Exp Parasitol 80:228–233

    Article  PubMed  CAS  Google Scholar 

  • Zhang YW, Fraser A, Balfour AH, Wreghitt TG, Gray JJ, Smith JE (1995) Serological reactivity against cyst and tachyzoite antigens of Toxoplasma gondii via FAST-ELISA. J Clin Pathol 48:908–911

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, Leeds University, Leeds, LS29JT, UK

    J. E. Smith, G. Biswas-Hughes & P. Appleford

  2. Department of Paediatrics, John Radcliffe Hospital, University of Oxford, Oxford, OX3 9DU, UK

    G. McNeil

  3. Department of Pathology, Albert Einstein College of Medicine, Yeshiva University, 1300 Morris Park Avenue, Bronx, New York, 10461, USA

    Y. W. Zhang

  4. Department of Life Sciences, Nottingham University, University Park, Nottingham, NG7 2RD, UK

    S. Dutton

Authors
  1. J. E. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. McNeil
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Y. W. Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. Dutton
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. G. Biswas-Hughes
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. P. Appleford
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Institute for Hygiene and Microbiology, University of Würzburg, Josef-Schneider-Str. 2, D-97080, Würzburg, Germany

    Uwe Gross

Rights and permissions

Reprints and permissions

Copyright information

© 1996 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Smith, J.E., McNeil, G., Zhang, Y.W., Dutton, S., Biswas-Hughes, G., Appleford, P. (1996). Serological Recognition of Toxoplasma gondii Cyst Antigens. In: Gross, U. (eds) Toxoplasma gondii. Current Topics in Microbiology and Immunology, vol 219. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-51014-4_7

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-51014-4_7

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-51016-8

  • Online ISBN: 978-3-642-51014-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation