Induction of Focal Brain Injury in Mice by Laser Irradiation at very Low Energy Level Following Injection of rose Bengal, a Photosensitizing Dye
Development of stroke therapy needs utilization of animal models able to simulate human pathology in a reproducible and physiologically relevant manner. An original approach for inducing brain infarction is based on a photochemical reaction between photosensitive dyes and light in the presence of molecular oxygen. Irradiation of photosensitive dyes such as fluorescein derivatives with light at an appropriate wavelength causes the production of reactive oxygen intermediates, mainly singlet oxygen and superoxide anion (Gandin et al., 1983). After injection of the dye into the circulation, irradiation of the vessels damages vascular endothelium leading to platelet aggregation and thrombotic infarction. . The technology was improved by Watson et al. (1985) by use of another dye, rose bengal, an efficient generator of oxygen radicals (Gandin et al., 1983). Irradiation was made using a filtered Xenon lamp through the intact calvarium of the ral, thereby rendering craniotomy unnecessary and ensuring the noninvasive character of the method.
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