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Induction of Focal Brain Injury in Mice by Laser Irradiation at very Low Energy Level Following Injection of rose Bengal, a Photosensitizing Dye

  • M. Boquillon
  • Jean Bralet
Conference paper

Abstract

Development of stroke therapy needs utilization of animal models able to simulate human pathology in a reproducible and physiologically relevant manner. An original approach for inducing brain infarction is based on a photochemical reaction between photosensitive dyes and light in the presence of molecular oxygen. Irradiation of photosensitive dyes such as fluorescein derivatives with light at an appropriate wavelength causes the production of reactive oxygen intermediates, mainly singlet oxygen and superoxide anion (Gandin et al., 1983). After injection of the dye into the circulation, irradiation of the vessels damages vascular endothelium leading to platelet aggregation and thrombotic infarction. . The technology was improved by Watson et al. (1985) by use of another dye, rose bengal, an efficient generator of oxygen radicals (Gandin et al., 1983). Irradiation was made using a filtered Xenon lamp through the intact calvarium of the ral, thereby rendering craniotomy unnecessary and ensuring the noninvasive character of the method.

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References

  1. Gandin E, Lion Y, Van de Vorst A (1983) Photochcm Photobiol 37: 271–278.CrossRefGoogle Scholar
  2. Bedcrson JB, Germano SM, Nishimura MC, Bartkowski HM (1986). Stroke 17:1304–1308.CrossRefGoogle Scholar
  3. Gromc JJ, Gojowczyk G, Hofmann W, Graham DI (1988) J Cereb Blood Flow Metab 8:89–95.CrossRefGoogle Scholar
  4. Watson BD, Dietrich WD, Busto R, Watchel MS. (1985). Ann. Neurol.17: 497–504.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • M. Boquillon
    • 1
  • Jean Bralet
    • 1
  1. 1.Laboratoire de PharmacodynamicFaculté de PharmacieDijonFrance

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