In Vitro Imaging of Tumors by Delayed Fluorescence
Laser-induced fluorescence was used to image tumors in vitro, which were marked with photosensitizers. Delayed detection of fluorescence following pulsed laser excitation allows to suppress autofluorescence background falling into the fluorescence band of porphyrin-based photosensitizer. This technique exploits the difference in fluorescence decay times of photosensitizers and average decay times of tissue autofluorescence. From delayed fluorescence spectra we infer that the ratio between photosensitizer signal and autofluorescence background can be improved by about one order of magnitude.
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