Characterization of Mott Cell Hybridomas from Autoimmune “Viable Motheaten” Mutant Mice

Abstract

Mott cells are abnormal plasma cells with discrete glycoprotein inclusions termed Russell bodies. These inclusions can be detected by their intense staining with periodic acid-Schiffs reagent (PAS). While Mott cells are present in low numbers in normal tissues (Lisco 1942), they are abundant in certain patients with multiple myeloma (Maldonado et al. 1966), trypanosomiasis (Mott 1905), and AIDS (Armstrong et al 1985). Mott cells can be found in experimental animals after hyperimmunization (White 1954) and in association with autoimmune disease of genetic origin (Alanen et al. 1985; Shultz et al. 1987). Although Mott cells have been recognized since 1890 (Russell 1890), neither the factors required for their development nor the role of these cells in normal and in diseased states have been determined. Mott cells are commonly found in lymphatic tissues from autoimmune, “viable motheaten” (me ^v) mutant mice after 5 weeks of age (Shultz et al 1987). In order to analyze the defect(s) associated with development of Mott cells, permanent cell lines (hybridomas) were established that retain the Mott cell phenotype; i.e., plasmacytoid cells containing large amounts of immunoglobulin (Ig) within Russell bodies.