Abstract
Embedding media that are miscible with water have played a significant role in light microscopy, particularly in the study of lipids. The embedding media used for this purpose have mostly been based upon polyethylene glycols (1); they do not have suitable properties for cutting the specially thin sections needed for electron microscopy. On the other hand, the embedding media that have been developed for thin sectioning are not miscible with water and have necessitated treatment of the specimens with a separate dehydrating agent, such as acetone or ethyl alcohol, before embedding (2, 3, 4). Gelatin has been proposed as a water-miscible embedding medium for electron microscopy (5, 6, 7), but it seems likely that the great shrinkage that occurs during hardening will preclude its general use.
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References
Miles, A. E. W., and J. E. Lindner: J. roy. Mier. Soc. 72, 199 (1952).
Newman, S. B., E. Borysko and M. Swerdlow: J. Res. nat. Bur. Stand. 43, 183 (1949).
Glauert, A. M., and R. H. Glauert: J. biophys. biochem. Cytol. 4, 191 (1958).
Kellenberger, E., W. Schwab and A. Ryter: Experientia (Basel) 12, 421 (1956).
Gilëv, V. P.: Electron microscopy: Proceedings of the Stockholm Congress. p. 113. Stockholm Almqvist and Wiksell 1957.
Gilëv, V. P.: J. Ultrastructural Res. 1, 349 (1958).
Fernandez-Morn, H., and J. B. Finean: J. biophys. biochem. Cytol. 3, 725 (1957).
Lee, H., and K. Neville• Epoxy resins. New York: McGraw Hill 1957.
Gibbons, I. R., and J. R. G. Bradfield: Electron Microscopy: Proceedings of the Stockholm Congress. p. 121. Stockholm Almqvist and Wiksell 1957.
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Gibbons, I.R. (1960). A water-miscible embedding resin for electron microscopy. In: Bargmann, W., Möllenstedt, G., Niehrs, H., Peters, D., Ruska, E., Wolpers, C. (eds) Vierter Internationaler Kongress für Elektronenmikroskopie / Fourth International Conference on Electron Microscopy / Quatrième Congrès International de Microscopie Électronique. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-49764-3_10
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DOI: https://doi.org/10.1007/978-3-642-49764-3_10
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