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Radioactive Isotopes in Physiology Diagnostics and Therapy / Künstliche Radioaktive Isotope in Physiologie Diagnostik und Therapie pp 1142–1185Cite as

Anwendung von radioaktiven Isotopen in der Enzymforschung

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Zusammenfassung

Die Methode der Markierung von Verbindungen, welche bei der Aufklärung von Stoffwechselwegen im komplexen System lebender Zellen so große Fortschritte gebracht hat, kann auch sinngemäß auf einzelne Enzymreaktionen angewendet werden, indem man das Substrat an bestimmten Atomen radioaktiv markiert und im Reaktionsprodukt Umfang und Ort der Markierung bestimmt. In vielen Fällen stellt diese Methode nur eine Variation einer Bestimmungsmethode dar, die vor einer anderen quantitativen Bestimmung bei Vorhandensein geeigneter Apparate Vorteile hinsichtlich der Schnelligkeit, Spezifität und Empfindlichkeit aufweisen kann. Eine Markierung des Phosphors in einem organischen Phosphorsäureester kann z. B. benutzt werden, um eine Spaltung durch Phosphatase auch in Gegenwart größerer Mengen von Fremdphosphor verschiedener Bindungsweise quantitativ zu untersuchen. Auch die vielen Nachweise und Bestimmungsmethoden bei der Überführung von Phosphor in eine andere Bindungsweise sind hier zu nennen. Man bedient sich hierbei vorzugsweise der Papierchromatographie, Papierelektrophorese oder Ionenaustauschchromatographie zur Trennung der vorliegenden Gemische. Die phosphorhaltigen Reaktionsprodukte lassen sich dann durch Radioautographie leicht sichtbar machen.

Zur Zeit Medicinska Nobelinstitutet, Biokemiska avdelningen, Stockholm.

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Authors
  1. Kurt Wallenfels
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  2. Horst Sund
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  1. Massachusetts General Hospital, Boston 14, Mass., USA

    Alan C. Aisenberg M. D., Ph. D.

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  36. Max-Planck-Institut für Virusforschung, Tübingen, Melanchthonstraße 36, Deutschland

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  37. Stiftung zur Erforschung der spinalen Kinderlähmung und der Multiplen Sklerose, Universitätskrankenhaus, Hamburg-Eppendorf, Martinistraße 52, Deutschland

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  40. The Royal Cancer Hospital, Fulham Road, London S. W. 3, UK

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  41. Physiologisch-Chemischen Instituts, Universität Mainz, Joh. Joachim Becher-Weg 13, Deutschland

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  42. Medizinische Universitäts-Poliklinik, Marburg/Lahn, Robert Koch-Str. 7a, Deutschland

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  43. Instituts für experimentelle Krebsforschung, Heidelberg, Voßstraße 3, Deutschland

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  44. z. Z. 17, Wing Terrace, Burlington, Mass., USA

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  45. Chemisches Institut der Universität, Bonn, Meckenheimer Allee 168, Deutschland

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  46. Seton Hall College of Medicine and Dentistry, Jersey City 4, N.J., USA

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  47. Laboratoire de Biochimie Générale et Comparée, Collège de France, Paris, Place Marcellin-Berthelot, Frankreich

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  48. Kantonsspital Zürich, Strahlenabteilung, Universitäts-Frauenklinik, Zürich, Schweiz

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  50. Columbia University, 630 West 168th Street, New York 32, N.Y., USA

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  58. Max Planck-Institut für Virusforschung, Tübingen, Melanchthonstraße 36, Deutschland

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  59. College of Physicians and Surgeons, Columbia University, 630 West 168th Street, New York 32, N.Y., USA

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  60. The Rockefeller Institute for Medical Research, 66th Street and York Avenue, New York 21, N.Y., USA

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  61. Chemisches Laboratorium der Universität, Freiburg i. Br., Albertstr. 21, Deutschland

    Horst Sund Dr.

  62. Department of Biochemistry, School of Medicine, University of California, San Francisco 22, Calif., USA

    H. Tarver Professor Dr.

  63. Chemischen Instituts, Universität Bonn, Meckenheimer Allee 168, Deutschland

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  64. Pharmakologisches Institut, Zürich, Schweiz, Gloriastraße 32

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  66. Physics Department, The Middlesex Hospital, Museum 833 Ext., 200 London W. 1, UK

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  67. I. Medizinische Universitätsklinik, München 15, Ziemssenstraße 1, Deutschland

    Hanns P. Wolff Professor Dr.

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Wallenfels, K., Sund, H. (1961). Anwendung von radioaktiven Isotopen in der Enzymforschung. In: Aisenberg, A.C., et al. Radioactive Isotopes in Physiology Diagnostics and Therapy / Künstliche Radioaktive Isotope in Physiologie Diagnostik und Therapie. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-49761-2_35

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  • DOI: https://doi.org/10.1007/978-3-642-49761-2_35

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