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Isotopes as a tool in biochemical analysis

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Radioactive Isotopes in Physiology Diagnostics and Therapy / Künstliche Radioaktive Isotope in Physiologie Diagnostik und Therapie

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Abstract

The solution of many biochemical problems is dependent upon the availability of accurate analytical methods for the quantitative estimation of individual inorganic or organic compounds present in the particular system under consideration. Many of these naturally occurring substances fall into distinct chemical groups whose members have similar molecular structure and show similar chemical behaviour. Few of the conventional methods of analytical chemistry are specific for any one compound and the success of these procedures is thus usually dependent upon the extent to which the mixture has been separated into individual substances before the actual quantitative estimations are made. For example, if the amounts of amino acids present in a protein hydrolysate are to be determined and if no preliminary separation of the amino acid mixture has been carried out, a set of about twenty quantitative reagents is required, each completely specific to one particular amino acid, characterising the side chain R of the structure RCH(NH2) COOH and totally unaffected by any other amino acids likely to be present in the mixture. If complete separation of the amino acid mixture has been carried out, a single general quantitative reagent for the —CH(NH2)COOH grouping is required.

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  1. J. K. Whitehead
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  1. Massachusetts General Hospital, Boston 14, Mass., USA

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Whitehead, J.K. (1961). Isotopes as a tool in biochemical analysis. In: Aisenberg, A.C., et al. Radioactive Isotopes in Physiology Diagnostics and Therapy / Künstliche Radioaktive Isotope in Physiologie Diagnostik und Therapie. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-49761-2_20

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  • DOI: https://doi.org/10.1007/978-3-642-49761-2_20

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