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Separation and Purification of Cord Factor (6,6′ Dimycoloyl Trehalose) from Wax C or from Mycolic Acids

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Investigation and Stimulation of Immunity in Cancer Patients

Part of the book series: Recent Results in Cancer Research ((RECENTCANCER,volume 47))

Abstract

Mycolic acids are among the most persistent contaminants of the toxic mycobacterial glycolipid 6,6′ dimycoloyl trehalose (cord factor). In the past, alternating multiple silicic acid and magnesium silicate column chromatography has been employed, not always successfully, to purify the glycolipid [5]. An ancillary problem which required a rapid method for bulk separation of certain derivatives of mycolic esters from the free acids led us to a disarmingly simple solution to both problems and ultimately to an improved method for isolation and purification of cord factor from wax C. Neutral lipids can be separated from quite large amounts of contaminant free carboxylic acids by adsorption chromatography on the free base form of diethylaminoethylcellulose (DEAE). The present paper describes the rationale and methods developed for cord factor purification.

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References

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Authors and Affiliations

  1. Department of Microbiology, University of Colorado School of Medicine, Denver, Colorado, USA

    M. B. Goren

  2. Division of Research, National Jewish Hospital and Research Center, University of Colorado School of Medicine, Denver, Colorado, USA

    Olga Brokl

Authors
  1. M. B. Goren
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  2. Olga Brokl
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Editor information

Editors and Affiliations

  1. Institut de Cancérologie et d’Immunogénétique, Hôpital Paul-Brousse, F-94, Villejuif, France

    Georges Mathé (Professor) (Professor)

  2. Children’s Cancer Research Foundation, 35 Binney Street, 02115, Boston, MA, USA

    Roy Weiner M.D.

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© 1974 Springer-Verlag Berlin · Heidelberg

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Goren, M.B., Brokl, O. (1974). Separation and Purification of Cord Factor (6,6′ Dimycoloyl Trehalose) from Wax C or from Mycolic Acids. In: Mathé, G., Weiner, R. (eds) Investigation and Stimulation of Immunity in Cancer Patients. Recent Results in Cancer Research, vol 47. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-49284-6_37

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  • DOI: https://doi.org/10.1007/978-3-642-49284-6_37

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-49286-0

  • Online ISBN: 978-3-642-49284-6

  • eBook Packages: Springer Book Archive

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