Abstract
Often a statement about a particular object, made as a result of an electron microscope examination, can only become convincing when backed up with numerical data obtained by image analysis. In addition, a statistical treatment of the measurements made can sometimes give rise to information which is not readily apparent in a qualitative description of the object concerned. Thus, morphometry entails methods of measurement as applied to morphological problems. An important area of morphometry is stereology, which provides the mathematical framework to enable one to create three-dimensional models from measurements carried out on two-dimensional images. Micrographs of sections, freeze-fracture replicas or freeze- or critical point-dried preparations often contain complicated spatial structures to which stereological methods must be applied.
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Literature
Agar AW, Alderson RH, Chescoe D (1974) Principles and practice of electron microscope operation. In: Glauert AM (ed) Practical methods in electron microscopy. Elsevier/North Holland, Amsterdam, pp 1–345
Bailey NTJ (1959) Statistical methods in biology. English Univ Press, London, pp 1–379
Ferguson J, Davis RW (1978) Quantitative electron microscopy of nucleic acids. In: Koehler JK (ed) Advanced techniques in biological electron microscopy, vol II. Springer, Berlin Heidelberg New York, pp 123–171
Gilbert N (1973) Biometrical interpretation. Oxford Univ Press, London, pp 1–125
Haug H (1976) Die verschiedenen Verfahren zur Werteerfassung in der biologischen Morphometrie und Stereologie. Microsc Acta 78: 197–220
Hoppe W (1977) Strukturanalyse mit Elektronenstrahlen (Elektronenmikroskopie). In: Hoppe W, Lohmann W, Markl H, Ziegler H (eds) Biophysik. Springer, Berlin Heidelberg New York, pp 67–87
Mishima Y, Matsunaka M (1976) Quantitative and three-dimensional analysis of dendritic cells and keratin cells in the human epidermis revealed by transmission and scanning electron microscopy. In: Yamada E, Mizurda V, Kurosima K, Nagano T (eds) Recent progress in electron microscopy of cells and tissues. Thieme, Stuttgart, pp 290–304
Weibel ER (1969) Stereological principles for morphometry in electron microscopic cytology. Int Rev Cytol 26: 235–302
Weibel ER (1979a) Stereological methods, vol I. Practical methods for biological morphometry. Academic Press, London New York, pp 1–415
Weibel ER ( 1979 b) Stereological methods, vol II. Theoretical foundations. Academic Press, London New York, pp 1–340
Willison JHM, Rowe AJ (1980) Resolution and quantitation in replicas and shadowed specimens. In: Glauert AM (ed) Practical methods in electron microscopy, vol VIII. Elsevier/ North Holland, Amsterdam, pp 245–282
Antranikian G, Klinner Ch, Kummel A, Schwanitz D, Zimmermann Th, Mayer F, Gottschalk G (1982) Purification of L-glutamate-dependent citrate lyase from Clostridium sphenoides and electron microscopic analysis of citrate lyase isolated from Rhodopseudomonas gelatinosa, Streptococcus diacetilactis and C. sphenoides. Eur J Biochem 126: 35–42
Home RW, Markham R (1973) Application of optical diffraction and image reconstruction techniques to electron micrographs. In: Glauert AM (ed) Practical methods in electron microscopy, vol I, part 2. Electron diffraction and optical techniques. Elsevier/North Holland, Amsterdam, pp 325–435
Baumeister W, Vogell W (eds) (1980) Electron microscopy at molecular dimensions; state of the art and strategies for the future. Springer, Berlin Heidelberg New York, pp 1–353
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© 1987 Springer-Verlag Berlin Heidelberg
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Robinson, D.G., Ehlers, U., Herken, R., Herrmann, B., Mayer, F., Schürmann, FW. (1987). Evaluation of Micrographs. In: Methods of Preparation for Electron Microscopy. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-48848-1_4
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DOI: https://doi.org/10.1007/978-3-642-48848-1_4
Publisher Name: Springer, Berlin, Heidelberg
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