Abstract
Plesiomonas shigelloides is a Gram-negative bacterium which has been implicated as an agent of human gastroenteritis [1] and is recognized as being an important cause of seafood-associated outbreaks [2, 3].A study in Japan ranked P. shigelloides first (66.7%) in a group of enteropathogens causing travellers’ diarrhoea. Septicaemia, cellulitis, meningitis, cholecystitis and endophthalmitis due to P. shigelloides have also been documented among immunocompromised patients. The mortality rate of these patients associated with Plesiomonas septicemia is high.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Khardori N, Fainstein V (1988) Aeromonas and Plesiomonas as etiological agents. Ann Rev Microbiol 42: 395–419
Rutala WA, Sarubbi FA, Finch CS, MacCormack JN, Steinkraus GE (1982) Oyster-associated outbreak of diarrhoeal disease possibly caused by Plesiomonas shigelloides ( Letter ). Lancet 1: 739
Tsukamoto T, Kinoshita Y, Shimada T, Sakazaki R (1978) Two epidemics of diarrhoeal disease possibly caused by Plesiomonas shigelloides. J Hy. 80: 275–280
Huq A, Aktar A, Chowdhury MAR, Sack DA (1991) Optimal growth temperature for isolation of Plesiomonas shigelloides using various selective and different agars. Can J Microbiol 37: 800–802
Olsen JE, Aabo S, Hill W, Notermans S, Wernars K, Granum PE, Popovic T, Rasmussen HN, Olsvik 0 (1995) Probes and polymerase chain reaction for detection of food-borne bacterial pathogens. Int J Food Microbiol 28: 1–78
Lou QY, Chong SKF, Fitzgerald JF, Siders JA, Allen SD, Lee CH (1997) Rapid and effective method for preparation of fecal specimens for PCR assays. J Clin Microbiol 35: 281–283
Gonzalez-Rey C, Svenson SB, Bravo L, Rosinsky J, Ciznar I, Krovacek K (2000) Specific detection of Plesiomonas shigelloides isolated from aquatic environments, animals and human diarrhoea) cases by PCR based on 23S rRNA gene. FEMS Immun Med Microbiol 29: 107–113
Cane PA, Cook P, Ratcliffe D, Mutimer D, Pillay D (1999) Use of real-time PCR and fluorimetry to detect lamivudine resistance-associated mutations in hepatitis B virus. Antimicrob Agents Chemo 43: 1600–1608
Yap EPH, McGee JOD (1991) Short PCR product yields improved by lower denaturation temperatures. Nucl Acids Res 19: 171–1714
Ririe KM, Rasmussen RP, Wittwer CT (1997) Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal Biochem 245: 154–160
Roche Molecular Biochemicals (1999) Decrease of fluorescent signal in the plateau phase of a LightCycler PCR hook effect. Roche Molecular Biochemicals Technical Note No. LC8/99
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Loh, J.P., Yap, E.P.H. (2002). Rapid and Specific Detection of Plesiomonas shigelloides Directly from Stool by LightCycler PCR. In: Reischl, U., Wittwer, C., Cockerill, F. (eds) Rapid Cycle Real-Time PCR — Methods and Applications. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-48351-6_15
Download citation
DOI: https://doi.org/10.1007/978-3-642-48351-6_15
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-48353-0
Online ISBN: 978-3-642-48351-6
eBook Packages: Springer Book Archive