Abstract
Long-term coculture of mononuclear cells of human umbilical cord blood with mouse skin derived 3T3 fibroblasts resulted in the development of human mast cells. These mast cells are morphologically and functionally mature, contain 1.4–2.8 μg histamine per 106 cells, and bear approximately 105FcεR1 per cell. The mast cells sensitized with human IgE released histamine upon challenge with anti-IgE. All of the cells contained human mast cell tryptase. Electron microscopic analysis of the cells showed that they were mature human mast cells and clearly different from basophilic granulocytes. Most of the mast cells contained some granules with regular crystalline arrays and both tryptase and chymase, thus resembling human skin mast cells.
When mononuclear cells of cord blood were seeded in a millicell insert which was placed on a 3T3 fibroblast monolayer, the number of mast cells that developed in the inserts was comparable to the number that developed following coculture of the same cord blood cells with 3T3 fibroblasts, indicating that direct contact between mast cell progenitors and fibroblasts is not essential. Recent observations that mast cells developed in the presence of concentrated culture super-natants of 3T3 fibroblasts without fibroblast feeder layer confirmed that soluble factors released from 3T3 fibroblasts are essential and sufficient for the differentiation of human mast cell progenitors in vitro.
This work was supported by Health and Human Services grants Al 10060, Al 27517, Al 20487, and CA 28834, and a research contract with DNAX Research Institute for Molecular and Cellular Biology. This is publication no. 3 from the La Jolla Institute of Allergy and Immunology.
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© 1991 Springer-Verlag Berlin Heidelberg
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Ishizaka, T., Furitsu, T., Inagaki, N., Dvorak, A.M. (1991). In Vitro Growth of Human Mast Cells and Their Functions. In: Ring, J., Przybilla, B. (eds) New Trends in Allergy III. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-46717-2_16
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DOI: https://doi.org/10.1007/978-3-642-46717-2_16
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