Construction of Human Suppressor T Cell Hybridomas That Produce Glycosylation Inhibiting Factors from Peripheral Blood Mononuclear Cells of Bee Venom Sensitive Patients

Abstract

Previous experiments on the regulation of the IgE response in BDF1 mice provided a method of generating antigen specific suppressor T cells in vitro from antigen primed T cell populations [1]. Spleen cells of BDF1 mice primed with alum absorbed ovalbumin (OVA) for a persistent IgE response produced IgE potentiating factors, i.e., glycosylated IgE binding factors (IgE-BF) after antigenic stimulation but did not contain antigen-specific suppressor cells [2]. However, propagation of the antigen-activated T cells by interleukin-2 (IL-2) in the presence of glycosylation inhibiting factor (GIF) [3] or synthetic phospholipase A2 inhibitor facilitated the generation of antigen specific T cells that produced their own GIF. Stimulation of the T cells with OVA-pulsed syngeneic macrophages resulted in the formation of an IgE suppressive factor, i.e., unglycosylated IgE-BF and GIF, the latter of which had affinity for OVA. Thus, T cell hybridomas were constructed from the OVA specific T cells. Upon antigenic stimulation, some of the hybridomas formed OVA-binding GIF, which suppressed the primary IgE response of BDF 1 mice to dinitrophenol (DNP)-OVA in a carrier specific manner [4] and which shared common antigenic structures with effector type, antigen specific, suppressor T cell factor (TseF) [5].