Abstract
Within the framework of studies to elucidate possible effects of the environmental pollutant SO2 on the biological activity of the rat liver carcinogen N-nitrosodimethylamine (NDMA), we performed several short-term in vitro and in vivo assays with the combination of both compounds. The alkaline filter elution technique was applied to measure genotoxicity as DNA-single strand breaks (SSB) in freshly isolated rat hepatocytes. Using this approach, we reported recently (1,2) that a reduction of about 20% of the in vitro-genotoxicity was observed with three N-nitrosamines (NDMA, N-nitrosoacetoxymethylmethylamine, Nnitrosomethylbenzylamine) when rats were pretreated with SO2 by inhalation prior to liver cell isolation. It was the aim of this study to investigate whether this effect can also be observed when both noxious compounds are simultaneously applied in vivo. In addition to genotoxicity, the hepatocytes isolated from the treated animals were assessed for viability (trypan blue exclusion), damage of cytoskeleton (bleb-formation) and energy-status (intracellular ATP-level).
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References
Pool, B.L. Klein, R.G. Monarca, S. Schmezer, P. and Zeller, W.J. (1988) Toxic and genotoxic effects of SO2 or NOx, alone and in combination with carcinogenic N-nitrosamines, in: N.H. Seemayer and W. Hadnagy (eds.), Environmental Hygiene, Springer-Verlag, 44–48.
Pool, B.L. Janowsky, I. Klein, P. Klein, R.G. Schmezer, P. Vogt-Leucht, G. and Zeller, J.W. (1988) Effects of SO2 or NOx, on toxic and genotoxic properties of chemical carcinogens. I. In vitro studies, Carcinogenesis 9, 1237–1245.
Pool, B.L. Eisenbrand, G. Preussmann, R. Schlehofer, J.R. Schmezer, P. Weber, H. and Wiessler, M. (1986) Detection of mutagens in bacteria and of DNA damage and amplified DNA sequences in mammalian cells as a systematic test strategy for elucidating biological activities of chemical carcinogens, Fd. Chem. Tox., 24, 685–691.
Liegibel, U.M. (1988) Vergleichende Bestimmung verschiedener ToxizitÖtsparameter an primären Rattenhepatozyten, Diplomarbeit an der Biologischen Fokuttät, Universität Heidelberg.
Zeller, J.W. Klein, R.G. Pool, B.L. and Schmezer, P. (1989) In vitro and in vivo studies of the carcinogenic, toxic and genotoxic activities of the air pollutants SO2 and NOx, separately and in combination with carcinogenic polycyclic aromatic hydrocarbons and Nnitrosamines IV, KFK-PEF 50, Band 2,463–474.
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© 1990 Springer-Verlag Berlin Heidelberg
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Schmezer, P., Klein, R.G., Liegibel, U.M., Zeller, W.J., Pool-Zobel, B.L. (1990). Combination Effects of N-Nitrosodimethylamine and SO2 . In: Seemayer, N.H., Hadnagy, W. (eds) Environmental Hygiene II. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-46712-7_7
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DOI: https://doi.org/10.1007/978-3-642-46712-7_7
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