Abstract
We studied the action of the restriction enzymes Eco R I, Eco R V, Eco R V*, Hind III, Kpn I, Bst E II, and Hpa I on different T-even phages’ DNAs. It was shown that under appropriate conditions all these enzymes were capable of splitting modified DNAs of T-even bacteriophages. T4 and T6 gene libraries were obtained, and clones with T4 genes coding for enzymes of DNA metabolism (30, 43, 44, 45, 46, 62, reg A, frd, td) were identified. Detailed restriction maps of these regions were constructed, and deletion and several other types of recombinant DNA produced. DNA ligase and dihydrofolate reductase genes were cloned into plasmids with a λ regulatory region, and strains with a different level of enzyme biosynthesis were selected.
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© 1987 Springer-Verlag Berlin Heidelberg
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Tanyashin, V.I. (1987). T-Even Bacteriophages: Structural Organization and Control of Expression of Genes of DNA Metabolism Enzymes. In: Organization and Function of the Eucaryotic Genome. Sitzungsberichte der Heidelberger Akademie der Wissenschaften, vol 1987/88 / 1987/3. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-46611-3_14
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DOI: https://doi.org/10.1007/978-3-642-46611-3_14
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-17798-2
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