Skip to main content
SpringerLink
Log in

Organization, Integration, and Transcription of Transforming Genes of Oncogenic Human Adenovirus Types 12 and 7

  • Chapter
The Molecular Biology of Adenoviruses 2

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 110))

Abstract

Thirty-one well-defined human adenovirus (Ad) serotypes are classified into five subgroups, A to E, according to their DNA-DNA homology (Green et al. 1979) as shown in Table 1. The members of subgroup A (Ad12, Ad18, and Ad31) are highly oncogenic, inducing tumors in newborn rodents at a high frequency within a few months; those of subgroup B (Ad3, Ad7, Ad14, Ad16, and Ad21) are weakly oncogenic, inducing tumors infrequently and only after longer periods; members of subgroups C to E are nononcogenic but transform rodent cells in culture (summarized by Green 1970; Tooze 1981). Transforming genes of the human adenoviruses Ad12 (Yano et al. 1977; Shiroki et al. 1977; Mak et al. 1979; Yamashita et al. 1982; Byrd et al. 1982), Ad31 (Yamashita and Fujinaga 1983), Ad3 (Dijkema et al. 1979), Ad7 (Sekikawa et al. 1978; Dijkema et al. 1979), Ad2 (Graham et al. 1974), Ad5 (Graham et al. 1974), and Ad4 (Yamashita, Nara, Suzuki, Ueno and Fujinaga, unpublished work) are located in the terminal region, early gene block E1 of the viral genome, and restriction DNA fragments containing these regions can transform rodent cells in culture. This review summarizes our studies on the organization, integration, and transcription of transforming genes of oncogenic Ad12 and Ad7.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Akusjärvi G, Pettersson U (1979) Sequence analysis of adenovirus DNA: complete nucleotide sequence of the spliced 5’ noncoding region of adenovirus 2 hexon messenger RNA. Cell 16: 841–850

    Article  PubMed  Google Scholar 

  • Alesträm P, Akusjürvi G, Perricaudet M, Mathews MB, Klessig D, Pettersson U (1980) The gene for polypeptide IX of adenovirus type 2 and its unspliced messenger RNA. Cell 19: 671–681

    Google Scholar 

  • Baker CC, Ziff EB (1979) Biogenesis, structure, and sites of encoding of the 5’ termini of adenovirus-2 mRNAs. Cold Spring Harbor Symp Quant Biol 44: 415–428

    Article  Google Scholar 

  • Baker CC, Ziff EB (1981) Promotors and heterogeneous 5’ termini of the messenger RNAs of adenovirus serotype 2. J Mol Biol 149: 189–221

    Article  PubMed  CAS  Google Scholar 

  • Baker CC, Hérissé J, Courtois G, Gallibert F, Ziff E (1979) Messenger RNA for the Ad2 DNA binding protein: DNA sequences encoding the first leader and heterogeneity at the mRNA 5’ end. Cell 18: 569–580

    Article  PubMed  CAS  Google Scholar 

  • Bernards R, Schrier PI, Bos JL, Van der Eb AJ (1983) Role of adenovirus type 5 and 12 early region 1 b tumor antigens in oncogenic transformation. Virology 126: 45–53

    Article  Google Scholar 

  • Bos JL, Polder LJ, Bernards R, Schrier PI, Van den Elsen PJ, Van der Eb AJ, Van Ormondt H (1981) The 2.2 kb E1B mRNA of human Ad12 and Ads codes for two tumor antigens starting at different AUG triplets. Cell 27: 121–131

    Article  PubMed  CAS  Google Scholar 

  • Breathnach R, Benoist C, O’Hare K, Gannon F, Chambon P (1978) Ovalbumin gene: evidence for a leader sequence in mRNA and DNA sequences at the exon-intron boundaries. Proc Natl Acad Sci USA 75: 4853–4857

    Article  PubMed  CAS  Google Scholar 

  • Byrd P, Chia W, Rigby PWJ, Gallimore PH (1982) Cloning of DNA fragments from the left end of the adenovirus type 12 genome: transformation by cloned early region 1. J Gen Virol 60: 279–293

    Article  PubMed  CAS  Google Scholar 

  • Chow LT, Roberts JM, Lewis JB, Broker TR (1977) A map of cytoplasmic RNA transcripts from lytic adenovirus type 2, determined by electron microscopy of RNA: DNA hybrids. Cell 11: 819–836

    Article  PubMed  CAS  Google Scholar 

  • Deuring R, Winterhoff U, Tamanoi F, Stabel S, Doerfler W (1981) Site of linkage between adenovirus type 12 and cell DNAs in hamster tumour line CLAC3. Nature 293: 81–84

    Article  PubMed  CAS  Google Scholar 

  • Dijkema R, Dekker BMM (1979) The inverted terminal repetition of the DNA of weakly oncogenic adenovirus type 7. Gene 8: 7–15

    Article  PubMed  CAS  Google Scholar 

  • Dijkema R, Dekker BMM, Van der Feltz MJM, Van der Eb AJ (1979) Transformation of primary rat kidney cells by DNA fragments of weakly oncogenic adenoviruses. J Virol 32: 943–950

    PubMed  CAS  Google Scholar 

  • Dijkema R, Dekker BMM, Van Ormondt H (1980a) The nucleotide sequence of the transforming BglII-H fragment of adenovirus type 7 DNA. Gene 9: 141–156

    Article  PubMed  CAS  Google Scholar 

  • Dijkema R, Dekker BMM, Van Ormondt H, de Waard A, Maat J, Boyer HW (1980b). Gene organization of the transforming region of weakly oncogenic adenovirus type 7: the EIA region. Gene 12: 287–299

    Article  PubMed  CAS  Google Scholar 

  • Dijkema R, Maat J, Dekker BMM, Van Ormondt H, Boyer HW (1981) The gene for polypeptide IX of human adenovirus type 7. Gene 13: 375–385

    Article  PubMed  CAS  Google Scholar 

  • Dijkema R, Dekker BMM, Van Ormondt H (1982) Gene organization of the transforming region of adenovirus type 7 DNA. Gene 18: 143–156

    Article  PubMed  CAS  Google Scholar 

  • Doerfler W (1982) Uptake, fixation, and expression of foreign DNA in mammalian cells: the organization of integrated adenovirus DNA sequences. In: Graf T, Jaenisch R (eds) Tumorviruses, Neoplastic Transformation and Differentiation. Springer, Berlin Heidelberg New York, pp 127–194 (Current topics in microbiology and immunology, Vol 101 )

    Google Scholar 

  • Frazer NW, Baker CC, Moore MA, Ziff EB (1982) Poly(A) sites of adenovirus serotype 2 transcription units. J Mol Biol 155: 207–233

    Article  Google Scholar 

  • Fujinaga K, Sekikawa K, Yamazaki H, Green M (1974) Analysis of multiple viral genome fragments in adenovirus 7-transformed hamster cells. Cold Spring Harbor Symp Quant Biol 39: 633–636

    Article  Google Scholar 

  • Fujinaga K, Sawada Y, Uemizu Y, Yamashita T, Shimojo H, Shiroki K, Sugisaki H, Sugimoto K, Takanami M (1979) Nucleotide sequences, integration, and transcription of the adenovirus 12 transforming genes. Cold Spring Harbor Symp Quant Biol 44: 519–532

    Article  Google Scholar 

  • Fujinaga K, Yoshida K, Sawada Y, Kimura T, Shimizu Y (1982) Organization and expression of oncogenic human adenovirus transforming genes. In: Proceedings of the 12th International Symposium of the Princess Takamatsu Cancer Research Fund. Primary and tertiary structure of nucleic acids and cancer research. Japan Scientific Society, Tokyo, pp 249–262

    Google Scholar 

  • Gahlmann R, Leisten R, Vardimon L, Doerfler W (1982) Patch homologies and the integration of adenovirus DNA in mammalian cells. EMBO J 1: 1101–1104

    PubMed  CAS  Google Scholar 

  • Gannon F, O’Hare K, Perrin F, LePennec JP, Benoist C, Cochet M, Breathnach R, Royal A, Garapin A, Cami B, Chambon P (1979) Organization and sequences at the 5’ end of a cloned complete ovalbumin gene. Nature 278: 428–434

    Article  PubMed  CAS  Google Scholar 

  • Gelb LD, Kohne DE, Martin MA (1971) Quantitation of simian virus 40 sequences in African green monkey, mouse and virus-transformed cell genomes. J Mol Biol 57: 129–145

    Article  PubMed  CAS  Google Scholar 

  • Gingeras TR, Sciaky D, Gelinas RE, Bing-Dong J, Yen CE, Kelly MM, Bullock PA, Parsons BL, O’Neill KE, Roberts RJ (1982) Nucleotide sequences from the adenovirus-2 genome. J Biol Chem 257: 13475–13491

    PubMed  CAS  Google Scholar 

  • Graham FL, Abrahams PJ, Mulder C, Heijnecker HL, Warnaar SO, de Vries FAJ, Fiers W, Van der Eb AJ (1974) Studies on in vitro transformation by DNA and DNA fragments of human adenoviruses and simian virus 40. Cold Spring Harbor Symp Quant Biol 39: 637–650

    Article  Google Scholar 

  • Green M (1970) Oncogenic viruses. Annu Rev Biochem 39: 701–756

    Article  PubMed  CAS  Google Scholar 

  • Green M, Mackey JK, Wold WSM, Rigden P (1979) Thirty-one human adenovirus serotypes (Ad1- Ad31) form five groups ( A—E) based upon DNA genome homologies. Virology 93: 481–492

    Article  PubMed  CAS  Google Scholar 

  • Hashimoto S, Pursley MH, Green M (1981a) Nucleotide sequences and mapping of novel heterogenous 5’-termini of adenovirus 2 early region 4 mRNA. Nucleic Acids Res 9: 1645–1689

    Article  Google Scholar 

  • Hashimoto S, Wold WSM, Brackmann KH, Green M (1981b) Nucleotide sequence of 5’ termini of adenovirus 2 early transforming region Ela and Elb messenger ribonucleic acids. Biochemistry 20: 6640–6647

    Article  PubMed  CAS  Google Scholar 

  • Kimura T, Sawada Y, Shinagawa M, Shimizu Y, Shiroki K, Shimojo H, Sugisaki H, Takanami M, Uemizu Y, Fujinaga K (1981) Nucleotide sequence of the transforming early region Elb of adenovirus type 12 DNA: structure and gene organization, and comparison with those of adenovirus type 5 DNA. Nucleic Acids Res 9: 6571–6589

    Article  PubMed  CAS  Google Scholar 

  • Maat J, Van Ormondt H (1979) The nucleotide sequence of the transforming HindIII-G fragment of adenovirus type 5 DNA. The region between map positions 4.5 (Hpal site) and 8.0 ( HindIII site ). Gene 6: 75–90

    Google Scholar 

  • Mak S, Mak I, Smiley JR, Graham FL (1979) Tumorigenicity and viral gene expression in rat cells transformed by Ad12 virions or by the EcoRI-C fragment of Ad12 DNA. Virology 98: 456–460

    Article  PubMed  CAS  Google Scholar 

  • Merregaert J, Van Emmelo J, Devos R, Porter A, Fellner P, and Fiers W (1978) The 3’-terminal nucleotide sequence of encephalomyocarditis virus RNA. Eur J Biochem 82: 55–63

    Article  PubMed  CAS  Google Scholar 

  • Mount SM (1982) A catalogue of splice junction sequences. Nucleic Acids Res 10: 459–471

    Article  PubMed  CAS  Google Scholar 

  • Osborne TF, Schell RE, Burch-Jaffe E, Berget SJ, Berk AJ (1981) Mapping a eukaryotic promotor: a DNA sequence required for in vivo expression of adenovirus pre-early functions. Proc Natl Acad Sci USA 78: 1381–1385

    Article  PubMed  CAS  Google Scholar 

  • Perricaudet M, Akusjärvi G, Virtanen A, Pettersson U (1979) Structure of two spliced mRNAs from transforming region of human subgroup C adenoviruses. Nature 281: 694–696

    Article  PubMed  CAS  Google Scholar 

  • Perricaudet M, le Moullec JM, Pettersson U (1980a) Predicted structure of two adenovirus tumor antigens. Proc Natl Acad Sci USA 77: 3778–3782

    Article  PubMed  CAS  Google Scholar 

  • Perricaudet M, le Moullec J-M, Tiollais P, Pettersson U (1980b) Structure of two adenovirus type 12 transforming polypeptides and their evolutionary implications. Nature 288: 174–176

    Article  PubMed  CAS  Google Scholar 

  • Pettersson U, Mathews MB (1977) The gene and messenger RNA for adenovirus polypeptide IX. Cell 12: 741–750

    Article  PubMed  CAS  Google Scholar 

  • Pribnow D (1975) Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter. Proc Natl Acad Sci USA 72: 784–788

    Article  PubMed  CAS  Google Scholar 

  • Proudfoot NJ (1979) Eukaryotic promoters? Nature 279: 376

    Article  PubMed  CAS  Google Scholar 

  • Proudfoot NJ, Brownlee GG (1976) Non-coding region sequences in eukaryotic messenger RNA. Nature 263: 211–214

    Article  PubMed  CAS  Google Scholar 

  • Saito I, Sato J, Handa H, Shiroki K, Shimojo H (1981) Mapping of RNAs transcribed from adenovirus type 12 early and VA RNA regions. Virology 114: 379–398

    Article  PubMed  CAS  Google Scholar 

  • Saito I, Shiroki K, Shimojo H (1983) mRNA species and proteins of adenovirus type 12 transforming regions: identification of proteins translated from multiple coding stretches in 2.2 kb region 1B mRNA in vitro and in vivo. Virology 127:272–289

    Google Scholar 

  • Sambrook J, Greene R, Stringer J, Mitchison T, Hu S-L, Botchan M (1979) Analysis of the sites of integration of viral DNA sequences in rat cells transformed by adenovirus 2 or SV40. Cold Spring Harbor Symp Quant Biol 44: 569–584

    Article  Google Scholar 

  • Sawada Y, Fujinaga K (1980) Mapping of adenovirus 12 mRNAs transcribed from transforming region. J Virol 36: 639–651

    PubMed  CAS  Google Scholar 

  • Sawada Y, Ojima S, Shimojo H, Shiroki K, Fujinaga K (1979) Transforming DNA sequences in rat cells transformed by DNA fragments of highly oncogenic human adenovirus type 12. J Virol 32: 379–385

    PubMed  CAS  Google Scholar 

  • Seeburg PH, Shine J, Martial JA, Baxter JD, Goodman HM (1977) Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone. Nature 270: 486–494

    Article  PubMed  CAS  Google Scholar 

  • Seif I, Khoury G, Dhar R (1979) BKV splice sequences based on analyses of preferred donor and acceptor sites. Nucleic Acids Res 6: 3387–3398

    Article  PubMed  CAS  Google Scholar 

  • Sekikawa K, Shiroki K, Shimojo H, Ojima S, Fujinaga K (1978) Transformation of rat cell line by an adenovirus 7 DNA fragment. Virology 88: 1–7

    Article  PubMed  CAS  Google Scholar 

  • Shiroki K, Handa H, Shimojo H, Yano S, Ojima S, Fujinaga K (1977) Establishment and characterization of rat cell lines transformed by restriction endonuclease fragments of adenovirus 12 DNA. Virology 82: 462–471

    Article  PubMed  CAS  Google Scholar 

  • Shiroki K, Shimojo H, Sawada Y, Uemizu Y, Fujinaga K (1979) Incomplete transformation of rat cells by a small fragment of adenovirus 12 DNA. Virology 95: 127–136

    Article  PubMed  CAS  Google Scholar 

  • Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517

    Article  PubMed  CAS  Google Scholar 

  • Sugisaki H, Sugimoto K, Takanami M, Shiroki K, Saito I, Shimojo H, Sawada Y, Uemizu Y, Uesugi S, Fujinaga K (1980) Structure and gene organization in the transforming HindIII-G fragment of Ad12. Cell 20: 777–786

    Article  PubMed  CAS  Google Scholar 

  • Tsujimoto Y, Hirose S, Tsuda M, Suzuki Y (1981) Promotor sequence of fibroin gene assigned by in vitro transcription system. Proc Natl Acad USA 78: 4838–4842

    Article  CAS  Google Scholar 

  • Tooze J (ed) (1981) Molecular biology of tumor viruses, 2nd edn part 2. DNA tumor viruses. Cold Spring Harbor, New York

    Google Scholar 

  • Van Beveren CP, Maat J, Dekker BMM, Van Ormondt H (1981) The nucleotide sequence of the gene for protein IVa 2 and of the 5’ leader segment of the major late mRNA of adenovirus type 5. Gene 16: 179–189

    Article  PubMed  Google Scholar 

  • Van den Elsen PJ, De Pater S, Houweling A, Van der Veer J, Van der Eb AJ (1982) The relationship between region Ela and Elb of human adenoviruses in cell transformation. Gene 18: 175–185

    Article  PubMed  Google Scholar 

  • Van der Eb AJ, Bernards R, Van den Elsen PJ, Bos JL, Schrier PI (1983) Studies on the role of adenovirus El genes in transformation and oncogenesis. Carcinogenesis (in press)

    Google Scholar 

  • Van Ormondt H, Hesper B (1983) Comparison of the nucleotide sequences of early region E1b DNA of human adenovirus type 12, 7 and 5 (subgroups A, B and C ). Gene 21: 217–226

    Article  PubMed  Google Scholar 

  • Van Ormondt H, Maat J, de Waard A, Van der Eb AJ (1978) The nucleotide sequence of the transforming Hpal-E fragment of adenovirus type 5 DNA. Gene 4: 309–328

    Article  PubMed  Google Scholar 

  • Van Ormondt H, Maat J, Van Bereren CP (1980a) The nucleotide sequence of the transforming early region El of adenovirus type 5 DNA. Gene 11: 299–309

    Article  PubMed  Google Scholar 

  • Van Ormondt H, Maat J, Dijkema R (1980b) Comparison of nucleotide sequences of the early Ela regions for subgroup A, B and C of human adenoviruses. Gene 12: 63–76

    Google Scholar 

  • Virtanen A, Pettersson U, le Moullec J-M, Tiollais P, Perricaudet M (1982) Different mRNAs from the transforming region of highly oncogenic and non-oncogenic human adenoviruses. Nature 295: 705–707

    Article  PubMed  CAS  Google Scholar 

  • Visser L, Van Maarschalkerweerd MW, Rozijn TH, Wassenaar ADC, Reemst AMCB, Sussenbach JS (1979) Viral DNA sequences in adenovirus-transformed cells. Cold Spring Harbor Symp Quant Biol 44: 541–550

    Article  Google Scholar 

  • Yamashita T, Fujinaga K (1983) Establishment and characterization of rat cell lines transformed by the left-end fragments of adenovirus type 31. Gann 74: 77–85

    PubMed  CAS  Google Scholar 

  • Yamashita T, Sugisaki H, Nara Y, Kanazawa S, Takanami M, Fujinaga K (1982) Transformation of rat 3Y1 cells by the cloned EcoRI-C fragment of human adenovirus type 12. Tumor Res 17: 9–22

    CAS  Google Scholar 

  • Yano S, Ojima S, Fujinaga K, Shiroki K, Shimojo H (1977) Transformation of a rat cell line by an adenovirus type 12 DNA fragment. Virology 82: 214–220

    Article  PubMed  CAS  Google Scholar 

  • Yoshida K, Fujinaga K (1980) Unique species of mRNA from adenovirus type 7 early region 1 in cells transformed by adenovirus type 7 DNA fragment. J Virol 36: 337–352

    PubMed  CAS  Google Scholar 

  • Zain S, Sambrook J, Roberts RJ, Keller W, Fried M, Dunn AR (1979) Nucleotide sequence analysis of the leader segments in a cloned copy of adenovirus 2 fiber mRNA. Cell 16: 851–861

    Article  PubMed  CAS  Google Scholar 

  • Ziff EB, Evans RM (1978) Coincidence of the promotor and capped 5’ terminus of RNA from the adenovirus 2 major late transcription unit. Cell 15: 1463–1475

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Molecular Biology, Cancer Research Institute, Sapporo Medical College S-1, W-17, Chuo-ku, Sapporo 060, Japan

    K. Fujinaga, K. Yoshida, T. Yamashita & Y. Shimizu

Authors
  1. K. Fujinaga
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Yoshida
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. Yamashita
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Y. Shimizu
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Institut für Genetik, Universität zu Köln, Weyertal 121, D-5000, Köln 41, Germany

    Walter Doerfler

Rights and permissions

Reprints and permissions

Copyright information

© 1984 Springer-Verlag Berlin · Heidelberg

About this chapter

Cite this chapter

Fujinaga, K., Yoshida, K., Yamashita, T., Shimizu, Y. (1984). Organization, Integration, and Transcription of Transforming Genes of Oncogenic Human Adenovirus Types 12 and 7. In: Doerfler, W. (eds) The Molecular Biology of Adenoviruses 2. Current Topics in Microbiology and Immunology, vol 110. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-46494-2_3

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-46494-2_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-46496-6

  • Online ISBN: 978-3-642-46494-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation